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A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity
ZnO nanoparticles (NPs) have previously been shown to exhibit selective cytotoxicity against certain types of cancerous cells suggesting their potential use in biomedical applications. In this study, we investigate the effect of surface modification of ZnO NPs on their cytotoxicity to both cancerous...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956064/ https://www.ncbi.nlm.nih.gov/pubmed/27486313 http://dx.doi.org/10.2147/NSA.S99747 |
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author | Wingett, Denise Louka, Panagiota Anders, Catherine B Zhang, Jianhui Punnoose, Alex |
author_facet | Wingett, Denise Louka, Panagiota Anders, Catherine B Zhang, Jianhui Punnoose, Alex |
author_sort | Wingett, Denise |
collection | PubMed |
description | ZnO nanoparticles (NPs) have previously been shown to exhibit selective cytotoxicity against certain types of cancerous cells suggesting their potential use in biomedical applications. In this study, we investigate the effect of surface modification of ZnO NPs on their cytotoxicity to both cancerous and primary T cells. Our results show that polyacrylic acid capping produces negatively charged ZnO NPs that are significantly more toxic compared to uncapped positively charged NPs of identical size and composition. In contrast, the greatest selectivity against cancerous cells relative to normal cells is observed with cationic NPs. In addition, differences in NP cytotoxicity inversely correlate with NP hydrodynamic size, propensity for aggregation, and dissolution profiles. The generation of reactive oxygen species (ROS) was also observed in the toxicity mechanism with anionic NPs generating higher levels of mitochondrial superoxide without appreciably affecting glutathione levels. Additional experiments evaluated the combined effects of charged ZnO NPs and nontoxic cationic or anionic CeO(2) NPs. Results show that the CeO(2) NPs offer protective effects against cytotoxicity from anionic ZnO NPs via antioxidant properties. Altogether, study data indicate that surface modification of NPs and resulting changes in their surface charge affect the level of intracellular ROS production, which can be ameliorated by the CeO(2) ROS scavenger, suggesting that ROS generation is a dominant mechanism of ZnO NP cytotoxicity. These findings demonstrate the importance of surface electrostatic properties for controlling NP toxicity and illustrate an approach for engineering NPs with desired properties for potential use in biological applications. |
format | Online Article Text |
id | pubmed-4956064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-49560642016-08-02 A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity Wingett, Denise Louka, Panagiota Anders, Catherine B Zhang, Jianhui Punnoose, Alex Nanotechnol Sci Appl Original Research ZnO nanoparticles (NPs) have previously been shown to exhibit selective cytotoxicity against certain types of cancerous cells suggesting their potential use in biomedical applications. In this study, we investigate the effect of surface modification of ZnO NPs on their cytotoxicity to both cancerous and primary T cells. Our results show that polyacrylic acid capping produces negatively charged ZnO NPs that are significantly more toxic compared to uncapped positively charged NPs of identical size and composition. In contrast, the greatest selectivity against cancerous cells relative to normal cells is observed with cationic NPs. In addition, differences in NP cytotoxicity inversely correlate with NP hydrodynamic size, propensity for aggregation, and dissolution profiles. The generation of reactive oxygen species (ROS) was also observed in the toxicity mechanism with anionic NPs generating higher levels of mitochondrial superoxide without appreciably affecting glutathione levels. Additional experiments evaluated the combined effects of charged ZnO NPs and nontoxic cationic or anionic CeO(2) NPs. Results show that the CeO(2) NPs offer protective effects against cytotoxicity from anionic ZnO NPs via antioxidant properties. Altogether, study data indicate that surface modification of NPs and resulting changes in their surface charge affect the level of intracellular ROS production, which can be ameliorated by the CeO(2) ROS scavenger, suggesting that ROS generation is a dominant mechanism of ZnO NP cytotoxicity. These findings demonstrate the importance of surface electrostatic properties for controlling NP toxicity and illustrate an approach for engineering NPs with desired properties for potential use in biological applications. Dove Medical Press 2016-07-15 /pmc/articles/PMC4956064/ /pubmed/27486313 http://dx.doi.org/10.2147/NSA.S99747 Text en © 2016 Wingett et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Wingett, Denise Louka, Panagiota Anders, Catherine B Zhang, Jianhui Punnoose, Alex A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity |
title | A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity |
title_full | A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity |
title_fullStr | A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity |
title_full_unstemmed | A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity |
title_short | A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity |
title_sort | role of zno nanoparticle electrostatic properties in cancer cell cytotoxicity |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956064/ https://www.ncbi.nlm.nih.gov/pubmed/27486313 http://dx.doi.org/10.2147/NSA.S99747 |
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