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A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity

ZnO nanoparticles (NPs) have previously been shown to exhibit selective cytotoxicity against certain types of cancerous cells suggesting their potential use in biomedical applications. In this study, we investigate the effect of surface modification of ZnO NPs on their cytotoxicity to both cancerous...

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Autores principales: Wingett, Denise, Louka, Panagiota, Anders, Catherine B, Zhang, Jianhui, Punnoose, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956064/
https://www.ncbi.nlm.nih.gov/pubmed/27486313
http://dx.doi.org/10.2147/NSA.S99747
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author Wingett, Denise
Louka, Panagiota
Anders, Catherine B
Zhang, Jianhui
Punnoose, Alex
author_facet Wingett, Denise
Louka, Panagiota
Anders, Catherine B
Zhang, Jianhui
Punnoose, Alex
author_sort Wingett, Denise
collection PubMed
description ZnO nanoparticles (NPs) have previously been shown to exhibit selective cytotoxicity against certain types of cancerous cells suggesting their potential use in biomedical applications. In this study, we investigate the effect of surface modification of ZnO NPs on their cytotoxicity to both cancerous and primary T cells. Our results show that polyacrylic acid capping produces negatively charged ZnO NPs that are significantly more toxic compared to uncapped positively charged NPs of identical size and composition. In contrast, the greatest selectivity against cancerous cells relative to normal cells is observed with cationic NPs. In addition, differences in NP cytotoxicity inversely correlate with NP hydrodynamic size, propensity for aggregation, and dissolution profiles. The generation of reactive oxygen species (ROS) was also observed in the toxicity mechanism with anionic NPs generating higher levels of mitochondrial superoxide without appreciably affecting glutathione levels. Additional experiments evaluated the combined effects of charged ZnO NPs and nontoxic cationic or anionic CeO(2) NPs. Results show that the CeO(2) NPs offer protective effects against cytotoxicity from anionic ZnO NPs via antioxidant properties. Altogether, study data indicate that surface modification of NPs and resulting changes in their surface charge affect the level of intracellular ROS production, which can be ameliorated by the CeO(2) ROS scavenger, suggesting that ROS generation is a dominant mechanism of ZnO NP cytotoxicity. These findings demonstrate the importance of surface electrostatic properties for controlling NP toxicity and illustrate an approach for engineering NPs with desired properties for potential use in biological applications.
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spelling pubmed-49560642016-08-02 A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity Wingett, Denise Louka, Panagiota Anders, Catherine B Zhang, Jianhui Punnoose, Alex Nanotechnol Sci Appl Original Research ZnO nanoparticles (NPs) have previously been shown to exhibit selective cytotoxicity against certain types of cancerous cells suggesting their potential use in biomedical applications. In this study, we investigate the effect of surface modification of ZnO NPs on their cytotoxicity to both cancerous and primary T cells. Our results show that polyacrylic acid capping produces negatively charged ZnO NPs that are significantly more toxic compared to uncapped positively charged NPs of identical size and composition. In contrast, the greatest selectivity against cancerous cells relative to normal cells is observed with cationic NPs. In addition, differences in NP cytotoxicity inversely correlate with NP hydrodynamic size, propensity for aggregation, and dissolution profiles. The generation of reactive oxygen species (ROS) was also observed in the toxicity mechanism with anionic NPs generating higher levels of mitochondrial superoxide without appreciably affecting glutathione levels. Additional experiments evaluated the combined effects of charged ZnO NPs and nontoxic cationic or anionic CeO(2) NPs. Results show that the CeO(2) NPs offer protective effects against cytotoxicity from anionic ZnO NPs via antioxidant properties. Altogether, study data indicate that surface modification of NPs and resulting changes in their surface charge affect the level of intracellular ROS production, which can be ameliorated by the CeO(2) ROS scavenger, suggesting that ROS generation is a dominant mechanism of ZnO NP cytotoxicity. These findings demonstrate the importance of surface electrostatic properties for controlling NP toxicity and illustrate an approach for engineering NPs with desired properties for potential use in biological applications. Dove Medical Press 2016-07-15 /pmc/articles/PMC4956064/ /pubmed/27486313 http://dx.doi.org/10.2147/NSA.S99747 Text en © 2016 Wingett et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Wingett, Denise
Louka, Panagiota
Anders, Catherine B
Zhang, Jianhui
Punnoose, Alex
A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity
title A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity
title_full A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity
title_fullStr A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity
title_full_unstemmed A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity
title_short A role of ZnO nanoparticle electrostatic properties in cancer cell cytotoxicity
title_sort role of zno nanoparticle electrostatic properties in cancer cell cytotoxicity
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956064/
https://www.ncbi.nlm.nih.gov/pubmed/27486313
http://dx.doi.org/10.2147/NSA.S99747
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