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Single-Shot Smartphone-Based Quantitative Phase Imaging Using a Distorted Grating

Blood testing has been used as an essential tool to diagnose diseases for decades. Recently, there has been a rapid developing trend in using Quantitative Phase Imaging (QPI) methods for blood cell screening. Compared to traditional blood testing techniques, QPI has the advantage of avoiding dyeing...

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Detalles Bibliográficos
Autores principales: Yang, Zhenyu, Zhan, Qiwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956142/
https://www.ncbi.nlm.nih.gov/pubmed/27441837
http://dx.doi.org/10.1371/journal.pone.0159596
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author Yang, Zhenyu
Zhan, Qiwen
author_facet Yang, Zhenyu
Zhan, Qiwen
author_sort Yang, Zhenyu
collection PubMed
description Blood testing has been used as an essential tool to diagnose diseases for decades. Recently, there has been a rapid developing trend in using Quantitative Phase Imaging (QPI) methods for blood cell screening. Compared to traditional blood testing techniques, QPI has the advantage of avoiding dyeing or staining the specimen, which may cause damage to the cells. However, most existing systems are bulky and costly, requiring experienced personnel to operate. This work demonstrates the integration of one QPI method onto a smartphone platform and the application of imaging red blood cells. The adopted QPI method is based on solving the Intensity Transport Equation (ITE) from two de-focused pupil images taken in one shot by the smartphone camera. The device demonstrates a system resolution of about 1 μm, and is ready to be used for 3D morphological study of red blood cells.
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spelling pubmed-49561422016-08-08 Single-Shot Smartphone-Based Quantitative Phase Imaging Using a Distorted Grating Yang, Zhenyu Zhan, Qiwen PLoS One Research Article Blood testing has been used as an essential tool to diagnose diseases for decades. Recently, there has been a rapid developing trend in using Quantitative Phase Imaging (QPI) methods for blood cell screening. Compared to traditional blood testing techniques, QPI has the advantage of avoiding dyeing or staining the specimen, which may cause damage to the cells. However, most existing systems are bulky and costly, requiring experienced personnel to operate. This work demonstrates the integration of one QPI method onto a smartphone platform and the application of imaging red blood cells. The adopted QPI method is based on solving the Intensity Transport Equation (ITE) from two de-focused pupil images taken in one shot by the smartphone camera. The device demonstrates a system resolution of about 1 μm, and is ready to be used for 3D morphological study of red blood cells. Public Library of Science 2016-07-21 /pmc/articles/PMC4956142/ /pubmed/27441837 http://dx.doi.org/10.1371/journal.pone.0159596 Text en © 2016 Yang, Zhan http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Yang, Zhenyu
Zhan, Qiwen
Single-Shot Smartphone-Based Quantitative Phase Imaging Using a Distorted Grating
title Single-Shot Smartphone-Based Quantitative Phase Imaging Using a Distorted Grating
title_full Single-Shot Smartphone-Based Quantitative Phase Imaging Using a Distorted Grating
title_fullStr Single-Shot Smartphone-Based Quantitative Phase Imaging Using a Distorted Grating
title_full_unstemmed Single-Shot Smartphone-Based Quantitative Phase Imaging Using a Distorted Grating
title_short Single-Shot Smartphone-Based Quantitative Phase Imaging Using a Distorted Grating
title_sort single-shot smartphone-based quantitative phase imaging using a distorted grating
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956142/
https://www.ncbi.nlm.nih.gov/pubmed/27441837
http://dx.doi.org/10.1371/journal.pone.0159596
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