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Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions

BACKGROUND: There is an urgent need to develop and incorporate novel behavioral tests in classically used preclinical pain models. Most rodent studies are based upon stimulus-evoked hindpaw measurements even though chronic pain is usually a day and night experience. Chronic pain is indeed a debilita...

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Autores principales: Pitzer, Claudia, Kuner, Rohini, Tappe-Theodor, Anke
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956152/
https://www.ncbi.nlm.nih.gov/pubmed/27306409
http://dx.doi.org/10.1177/1744806916656635
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author Pitzer, Claudia
Kuner, Rohini
Tappe-Theodor, Anke
author_facet Pitzer, Claudia
Kuner, Rohini
Tappe-Theodor, Anke
author_sort Pitzer, Claudia
collection PubMed
description BACKGROUND: There is an urgent need to develop and incorporate novel behavioral tests in classically used preclinical pain models. Most rodent studies are based upon stimulus-evoked hindpaw measurements even though chronic pain is usually a day and night experience. Chronic pain is indeed a debilitating condition that influences the sociability and the ability for voluntary tasks, but the relevant behavioral readouts for these aspects are mostly under-represented in the literature. Moreover, we lack standardization in most behavioral paradigms to guarantee reproducibility and ensure adequate discussion between different studies. This concerns not only the combination, application, and duration of particular behavioral tasks but also the effects of different housing conditions implicating social isolation. RESULTS: Our aim was to thoroughly characterize the classically used spared nerve injury model for 12 weeks following surgery. We used a portfolio of classical stimulus-evoked response measurements, detailed gait analysis with two different measuring systems (Dynamic weight bearing (DWB) system and CatWalk), as well as observer-independent voluntary wheel running and home cage monitoring (Laboras system). Additionally, we analyzed the effects of social isolation in all behavioral tasks. We found that evoked hypersensitivity temporally matched changes in static gait parameters, whereas some dynamic gait parameters were changed in a time-dependent manner. Interestingly, voluntary wheel running behavior was not affected in spared nerve injury mice but by social isolation. Besides a reduced climbing activity, spared nerve injury mice did not showed tremendous alterations in the home cage activity. CONCLUSION: This is the first longitudinal study providing detailed insights into various voluntary behavioral parameters related to pain and highlights the importance of social environment on spontaneous non-evoked behaviors in a mouse model of chronic neuropathy. Our results provide fundamental considerations for future experimental planning and discussion of pain-related behavioral changes.
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spelling pubmed-49561522016-08-12 Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions Pitzer, Claudia Kuner, Rohini Tappe-Theodor, Anke Mol Pain Research Article BACKGROUND: There is an urgent need to develop and incorporate novel behavioral tests in classically used preclinical pain models. Most rodent studies are based upon stimulus-evoked hindpaw measurements even though chronic pain is usually a day and night experience. Chronic pain is indeed a debilitating condition that influences the sociability and the ability for voluntary tasks, but the relevant behavioral readouts for these aspects are mostly under-represented in the literature. Moreover, we lack standardization in most behavioral paradigms to guarantee reproducibility and ensure adequate discussion between different studies. This concerns not only the combination, application, and duration of particular behavioral tasks but also the effects of different housing conditions implicating social isolation. RESULTS: Our aim was to thoroughly characterize the classically used spared nerve injury model for 12 weeks following surgery. We used a portfolio of classical stimulus-evoked response measurements, detailed gait analysis with two different measuring systems (Dynamic weight bearing (DWB) system and CatWalk), as well as observer-independent voluntary wheel running and home cage monitoring (Laboras system). Additionally, we analyzed the effects of social isolation in all behavioral tasks. We found that evoked hypersensitivity temporally matched changes in static gait parameters, whereas some dynamic gait parameters were changed in a time-dependent manner. Interestingly, voluntary wheel running behavior was not affected in spared nerve injury mice but by social isolation. Besides a reduced climbing activity, spared nerve injury mice did not showed tremendous alterations in the home cage activity. CONCLUSION: This is the first longitudinal study providing detailed insights into various voluntary behavioral parameters related to pain and highlights the importance of social environment on spontaneous non-evoked behaviors in a mouse model of chronic neuropathy. Our results provide fundamental considerations for future experimental planning and discussion of pain-related behavioral changes. SAGE Publications 2016-06-15 /pmc/articles/PMC4956152/ /pubmed/27306409 http://dx.doi.org/10.1177/1744806916656635 Text en © The Author(s) 2016 http://creativecommons.org/licenses/by-nc/3.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Research Article
Pitzer, Claudia
Kuner, Rohini
Tappe-Theodor, Anke
Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions
title Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions
title_full Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions
title_fullStr Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions
title_full_unstemmed Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions
title_short Voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions
title_sort voluntary and evoked behavioral correlates in neuropathic pain states under different social housing conditions
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956152/
https://www.ncbi.nlm.nih.gov/pubmed/27306409
http://dx.doi.org/10.1177/1744806916656635
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