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Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment

The lack of a robust anticancer drug screening system to monitor patients during treatment delays realization of personalized treatment. We demonstrate an efficient approach to evaluate drug response using patient-derived circulating tumor cell (CTC) cultures obtained from liquid biopsy. Custom micr...

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Autores principales: Khoo, Bee Luan, Grenci, Gianluca, Jing, Tengyang, Lim, Ying Bena, Lee, Soo Chin, Thiery, Jean Paul, Han, Jongyoon, Lim, Chwee Teck
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956185/
https://www.ncbi.nlm.nih.gov/pubmed/27453941
http://dx.doi.org/10.1126/sciadv.1600274
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author Khoo, Bee Luan
Grenci, Gianluca
Jing, Tengyang
Lim, Ying Bena
Lee, Soo Chin
Thiery, Jean Paul
Han, Jongyoon
Lim, Chwee Teck
author_facet Khoo, Bee Luan
Grenci, Gianluca
Jing, Tengyang
Lim, Ying Bena
Lee, Soo Chin
Thiery, Jean Paul
Han, Jongyoon
Lim, Chwee Teck
author_sort Khoo, Bee Luan
collection PubMed
description The lack of a robust anticancer drug screening system to monitor patients during treatment delays realization of personalized treatment. We demonstrate an efficient approach to evaluate drug response using patient-derived circulating tumor cell (CTC) cultures obtained from liquid biopsy. Custom microfabricated tapered microwells were integrated with microfluidics to allow robust formation of CTC clusters without pre-enrichment and subsequent drug screening in situ. Rapid feedback after 2 weeks promotes immediate intervention upon detection of drug resistance or tolerance. The procedure was clinically validated with blood samples (n = 73) from 55 patients with early-stage, newly diagnosed, locally advanced, or refractory metastatic breast cancer. Twenty-four of these samples were used for drug evaluation. Cluster formation potential correlated inversely with increased drug concentration and therapeutic treatment. This new and robust liquid biopsy technique can potentially evaluate patient prognosis with CTC clusters during treatment and provide a noninvasive and inexpensive assessment that can guide drug discovery development or therapeutic choices for personalized treatment.
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spelling pubmed-49561852016-07-22 Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment Khoo, Bee Luan Grenci, Gianluca Jing, Tengyang Lim, Ying Bena Lee, Soo Chin Thiery, Jean Paul Han, Jongyoon Lim, Chwee Teck Sci Adv Research Articles The lack of a robust anticancer drug screening system to monitor patients during treatment delays realization of personalized treatment. We demonstrate an efficient approach to evaluate drug response using patient-derived circulating tumor cell (CTC) cultures obtained from liquid biopsy. Custom microfabricated tapered microwells were integrated with microfluidics to allow robust formation of CTC clusters without pre-enrichment and subsequent drug screening in situ. Rapid feedback after 2 weeks promotes immediate intervention upon detection of drug resistance or tolerance. The procedure was clinically validated with blood samples (n = 73) from 55 patients with early-stage, newly diagnosed, locally advanced, or refractory metastatic breast cancer. Twenty-four of these samples were used for drug evaluation. Cluster formation potential correlated inversely with increased drug concentration and therapeutic treatment. This new and robust liquid biopsy technique can potentially evaluate patient prognosis with CTC clusters during treatment and provide a noninvasive and inexpensive assessment that can guide drug discovery development or therapeutic choices for personalized treatment. American Association for the Advancement of Science 2016-07-13 /pmc/articles/PMC4956185/ /pubmed/27453941 http://dx.doi.org/10.1126/sciadv.1600274 Text en Copyright © 2016, The Authors http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Khoo, Bee Luan
Grenci, Gianluca
Jing, Tengyang
Lim, Ying Bena
Lee, Soo Chin
Thiery, Jean Paul
Han, Jongyoon
Lim, Chwee Teck
Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment
title Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment
title_full Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment
title_fullStr Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment
title_full_unstemmed Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment
title_short Liquid biopsy and therapeutic response: Circulating tumor cell cultures for evaluation of anticancer treatment
title_sort liquid biopsy and therapeutic response: circulating tumor cell cultures for evaluation of anticancer treatment
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956185/
https://www.ncbi.nlm.nih.gov/pubmed/27453941
http://dx.doi.org/10.1126/sciadv.1600274
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