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dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis
Proper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956209/ https://www.ncbi.nlm.nih.gov/pubmed/27442438 http://dx.doi.org/10.1371/journal.pgen.1006204 |
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author | Brás-Pereira, Catarina Potier, Delphine Jacobs, Jelle Aerts, Stein Casares, Fernando Janody, Florence |
author_facet | Brás-Pereira, Catarina Potier, Delphine Jacobs, Jelle Aerts, Stein Casares, Fernando Janody, Florence |
author_sort | Brás-Pereira, Catarina |
collection | PubMed |
description | Proper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which coordinates cell fate determination, cell cycle and tissue morphogenesis. Here we show that during Drosophila retinogenesis, the retinal determination gene dachshund (dac) is not only a target of the Hh signaling pathway, but is also a modulator of its activity. Using developmental genetics techniques, we demonstrate that dac enhances Hh signaling by promoting the accumulation of the Gli transcription factor Cubitus interruptus (Ci) parallel to or downstream of fused. In the absence of dac, all Hh-mediated events associated to the morphogenetic furrow are delayed. One of the consequences is that, posterior to the furrow, dac- cells cannot activate a Roadkill-Cullin3 negative feedback loop that attenuates Hh signaling and which is necessary for retinal cells to continue normal differentiation. Therefore, dac is part of an essential positive feedback loop in the Hh pathway, guaranteeing the speed and the accuracy of Drosophila retinogenesis. |
format | Online Article Text |
id | pubmed-4956209 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49562092016-08-08 dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis Brás-Pereira, Catarina Potier, Delphine Jacobs, Jelle Aerts, Stein Casares, Fernando Janody, Florence PLoS Genet Research Article Proper organ patterning depends on a tight coordination between cell proliferation and differentiation. The patterning of Drosophila retina occurs both very fast and with high precision. This process is driven by the dynamic changes in signaling activity of the conserved Hedgehog (Hh) pathway, which coordinates cell fate determination, cell cycle and tissue morphogenesis. Here we show that during Drosophila retinogenesis, the retinal determination gene dachshund (dac) is not only a target of the Hh signaling pathway, but is also a modulator of its activity. Using developmental genetics techniques, we demonstrate that dac enhances Hh signaling by promoting the accumulation of the Gli transcription factor Cubitus interruptus (Ci) parallel to or downstream of fused. In the absence of dac, all Hh-mediated events associated to the morphogenetic furrow are delayed. One of the consequences is that, posterior to the furrow, dac- cells cannot activate a Roadkill-Cullin3 negative feedback loop that attenuates Hh signaling and which is necessary for retinal cells to continue normal differentiation. Therefore, dac is part of an essential positive feedback loop in the Hh pathway, guaranteeing the speed and the accuracy of Drosophila retinogenesis. Public Library of Science 2016-07-21 /pmc/articles/PMC4956209/ /pubmed/27442438 http://dx.doi.org/10.1371/journal.pgen.1006204 Text en © 2016 Brás-Pereira et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Brás-Pereira, Catarina Potier, Delphine Jacobs, Jelle Aerts, Stein Casares, Fernando Janody, Florence dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title | dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title_full | dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title_fullStr | dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title_full_unstemmed | dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title_short | dachshund Potentiates Hedgehog Signaling during Drosophila Retinogenesis |
title_sort | dachshund potentiates hedgehog signaling during drosophila retinogenesis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956209/ https://www.ncbi.nlm.nih.gov/pubmed/27442438 http://dx.doi.org/10.1371/journal.pgen.1006204 |
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