Cargando…

Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro

Rinsing the mouth with sodium chloride (NaCl) solution is believed to promote healthy gum and improve oral ulcer healing. Scientific evidence to support this assumption is, however, lacking. This study aims to investigate the effect and clarify underlying mechanisms of short-term rinsing with NaCl o...

Descripción completa

Detalles Bibliográficos
Autores principales: Huynh, Nam Cong-Nhat, Everts, Vincent, Leethanakul, Chidchanok, Pavasant, Prasit, Ampornaramveth, Ruchanee Salingcarnboriboon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956236/
https://www.ncbi.nlm.nih.gov/pubmed/27441729
http://dx.doi.org/10.1371/journal.pone.0159843
_version_ 1782444005418598400
author Huynh, Nam Cong-Nhat
Everts, Vincent
Leethanakul, Chidchanok
Pavasant, Prasit
Ampornaramveth, Ruchanee Salingcarnboriboon
author_facet Huynh, Nam Cong-Nhat
Everts, Vincent
Leethanakul, Chidchanok
Pavasant, Prasit
Ampornaramveth, Ruchanee Salingcarnboriboon
author_sort Huynh, Nam Cong-Nhat
collection PubMed
description Rinsing the mouth with sodium chloride (NaCl) solution is believed to promote healthy gum and improve oral ulcer healing. Scientific evidence to support this assumption is, however, lacking. This study aims to investigate the effect and clarify underlying mechanisms of short-term rinsing with NaCl on human gingival fibroblast (hGFs) wound healing. Isolated primary hGFs and human normal oral keratinocytes (hNOKs) were rinsed with 0–7.2% NaCl for 2 min, 3 times a day. Scratch-tests, trans-well migration assays and MTT activity were performed. mRNA expression was assessed of type-I collagen, fibronectin and FAK. Changes in FAK and F-actin were detected by immunofluorescence. KCl, NaH(2)PO(4), KH(2)PO(4) were used to clarify the molecules involved. Rinsing with 0.9–1.8% NaCl significantly promoted hGFs cell migration but not proliferation. However, it had no effect on hNOKs. Rinsing with 1.8% NaCl significantly up-regulated the expression of type-I collagen and fibronectin. FAK and F-actin, molecules responsible for cytoskeleton re-organization and cell migration, were also up-regulated. Cl(-) seemed to be essential since rinsing with KCl resulted in a similar effect as noted with NaCl. In conclusion, short-term rinsing with NaCl promoted hGFs migration, and increased the expression of extracellular matrix as well as cytoskeletal proteins. These data strongly support the long held belief in the benefits of using NaCl mouth-rinse.
format Online
Article
Text
id pubmed-4956236
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-49562362016-08-08 Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro Huynh, Nam Cong-Nhat Everts, Vincent Leethanakul, Chidchanok Pavasant, Prasit Ampornaramveth, Ruchanee Salingcarnboriboon PLoS One Research Article Rinsing the mouth with sodium chloride (NaCl) solution is believed to promote healthy gum and improve oral ulcer healing. Scientific evidence to support this assumption is, however, lacking. This study aims to investigate the effect and clarify underlying mechanisms of short-term rinsing with NaCl on human gingival fibroblast (hGFs) wound healing. Isolated primary hGFs and human normal oral keratinocytes (hNOKs) were rinsed with 0–7.2% NaCl for 2 min, 3 times a day. Scratch-tests, trans-well migration assays and MTT activity were performed. mRNA expression was assessed of type-I collagen, fibronectin and FAK. Changes in FAK and F-actin were detected by immunofluorescence. KCl, NaH(2)PO(4), KH(2)PO(4) were used to clarify the molecules involved. Rinsing with 0.9–1.8% NaCl significantly promoted hGFs cell migration but not proliferation. However, it had no effect on hNOKs. Rinsing with 1.8% NaCl significantly up-regulated the expression of type-I collagen and fibronectin. FAK and F-actin, molecules responsible for cytoskeleton re-organization and cell migration, were also up-regulated. Cl(-) seemed to be essential since rinsing with KCl resulted in a similar effect as noted with NaCl. In conclusion, short-term rinsing with NaCl promoted hGFs migration, and increased the expression of extracellular matrix as well as cytoskeletal proteins. These data strongly support the long held belief in the benefits of using NaCl mouth-rinse. Public Library of Science 2016-07-21 /pmc/articles/PMC4956236/ /pubmed/27441729 http://dx.doi.org/10.1371/journal.pone.0159843 Text en © 2016 Huynh et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Huynh, Nam Cong-Nhat
Everts, Vincent
Leethanakul, Chidchanok
Pavasant, Prasit
Ampornaramveth, Ruchanee Salingcarnboriboon
Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro
title Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro
title_full Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro
title_fullStr Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro
title_full_unstemmed Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro
title_short Rinsing with Saline Promotes Human Gingival Fibroblast Wound Healing In Vitro
title_sort rinsing with saline promotes human gingival fibroblast wound healing in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4956236/
https://www.ncbi.nlm.nih.gov/pubmed/27441729
http://dx.doi.org/10.1371/journal.pone.0159843
work_keys_str_mv AT huynhnamcongnhat rinsingwithsalinepromoteshumangingivalfibroblastwoundhealinginvitro
AT evertsvincent rinsingwithsalinepromoteshumangingivalfibroblastwoundhealinginvitro
AT leethanakulchidchanok rinsingwithsalinepromoteshumangingivalfibroblastwoundhealinginvitro
AT pavasantprasit rinsingwithsalinepromoteshumangingivalfibroblastwoundhealinginvitro
AT ampornaramvethruchaneesalingcarnboriboon rinsingwithsalinepromoteshumangingivalfibroblastwoundhealinginvitro