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CD317 Promotes the survival of cancer cells through apoptosis-inducing factor
BACKGROUND: Low nutrient environment is a major obstacle to solid tumor growth. However, many tumors have developed adaptive mechanisms to circumvent the requirement for exogenous growth factors. METHODS: Here we used siRNA interference or plasmid transfection techniques to knockdown or enhance CD31...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957287/ https://www.ncbi.nlm.nih.gov/pubmed/27444183 http://dx.doi.org/10.1186/s13046-016-0391-2 |
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author | Li, Xin Zhang, Guizhong Chen, Qian Lin, Yingxue Li, Junxin Ruan, Qingguo Chen, Youhai Yu, Guang Wan, Xiaochun |
author_facet | Li, Xin Zhang, Guizhong Chen, Qian Lin, Yingxue Li, Junxin Ruan, Qingguo Chen, Youhai Yu, Guang Wan, Xiaochun |
author_sort | Li, Xin |
collection | PubMed |
description | BACKGROUND: Low nutrient environment is a major obstacle to solid tumor growth. However, many tumors have developed adaptive mechanisms to circumvent the requirement for exogenous growth factors. METHODS: Here we used siRNA interference or plasmid transfection techniques to knockdown or enhance CD317 expression respectively, in mammalian cancer cells, and subjected these CD317-manipulated cells to serum deprivation to study the role of CD317 on stress-induced apoptosis and the underlying mechanism. RESULTS: We report that CD317, an innate immune gene overexpressed in human cancers, protected cancer cells against serum deprivation-induced apoptosis. In tumor cells, loss of CD317 markedly enhanced their susceptibility to serum deprivation-induced apoptosis with no effect on autophagy or caspase activation, indicating an autophagy- and caspase-independent mechanism of CD317 function. Importantly, CD317 knockdown in serum-deprived tumor cells impaired mitochondria function and subsequently promoted apoptosis-inducing factor (AIF) release and nuclear translocation but had little effect on mitochondrial and cytoplasmic distributions of cytochrome C, a pro-apoptotic factor released from mitochondria that initiates caspase processing in response to death stimuli. Furthermore, overexpression of CD317 in HEK293T cells inhibits serum deprivation-induced apoptosis as well as the release and nuclear accumulation of AIF. CONCLUSION: Our data suggest that CD317 functions as an anti-apoptotic factor through the mitochondria-AIF axis in malnourished condition and may serve as a potential drug target for cancer therapy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13046-016-0391-2) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4957287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49572872016-07-23 CD317 Promotes the survival of cancer cells through apoptosis-inducing factor Li, Xin Zhang, Guizhong Chen, Qian Lin, Yingxue Li, Junxin Ruan, Qingguo Chen, Youhai Yu, Guang Wan, Xiaochun J Exp Clin Cancer Res Research BACKGROUND: Low nutrient environment is a major obstacle to solid tumor growth. However, many tumors have developed adaptive mechanisms to circumvent the requirement for exogenous growth factors. METHODS: Here we used siRNA interference or plasmid transfection techniques to knockdown or enhance CD317 expression respectively, in mammalian cancer cells, and subjected these CD317-manipulated cells to serum deprivation to study the role of CD317 on stress-induced apoptosis and the underlying mechanism. RESULTS: We report that CD317, an innate immune gene overexpressed in human cancers, protected cancer cells against serum deprivation-induced apoptosis. In tumor cells, loss of CD317 markedly enhanced their susceptibility to serum deprivation-induced apoptosis with no effect on autophagy or caspase activation, indicating an autophagy- and caspase-independent mechanism of CD317 function. Importantly, CD317 knockdown in serum-deprived tumor cells impaired mitochondria function and subsequently promoted apoptosis-inducing factor (AIF) release and nuclear translocation but had little effect on mitochondrial and cytoplasmic distributions of cytochrome C, a pro-apoptotic factor released from mitochondria that initiates caspase processing in response to death stimuli. Furthermore, overexpression of CD317 in HEK293T cells inhibits serum deprivation-induced apoptosis as well as the release and nuclear accumulation of AIF. CONCLUSION: Our data suggest that CD317 functions as an anti-apoptotic factor through the mitochondria-AIF axis in malnourished condition and may serve as a potential drug target for cancer therapy. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13046-016-0391-2) contains supplementary material, which is available to authorized users. BioMed Central 2016-07-22 /pmc/articles/PMC4957287/ /pubmed/27444183 http://dx.doi.org/10.1186/s13046-016-0391-2 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Li, Xin Zhang, Guizhong Chen, Qian Lin, Yingxue Li, Junxin Ruan, Qingguo Chen, Youhai Yu, Guang Wan, Xiaochun CD317 Promotes the survival of cancer cells through apoptosis-inducing factor |
title | CD317 Promotes the survival of cancer cells through apoptosis-inducing factor |
title_full | CD317 Promotes the survival of cancer cells through apoptosis-inducing factor |
title_fullStr | CD317 Promotes the survival of cancer cells through apoptosis-inducing factor |
title_full_unstemmed | CD317 Promotes the survival of cancer cells through apoptosis-inducing factor |
title_short | CD317 Promotes the survival of cancer cells through apoptosis-inducing factor |
title_sort | cd317 promotes the survival of cancer cells through apoptosis-inducing factor |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957287/ https://www.ncbi.nlm.nih.gov/pubmed/27444183 http://dx.doi.org/10.1186/s13046-016-0391-2 |
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