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Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia
In this study, we identified the phlebotomine sandfly vectors involved in the transmission of American Cutaneous Leishmaniasis (ACL) in Assis Brasil, Acre, Brazil, which is located on the Brazil-Peru-Bolivia frontier. The genotyping of Leishmania in phlebotomines was performed using polymerase chain...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Instituto Oswaldo Cruz, Ministério da Saúde
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957494/ https://www.ncbi.nlm.nih.gov/pubmed/27304023 http://dx.doi.org/10.1590/0074-02760160054 |
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author | Teles, Carolina Bioni Garcia dos Santos, Ana Paula de Azevedo Freitas, Rui Alves de Oliveira, Arley Faria José Ogawa, Guilherme Maerschner Rodrigues, Moreno Souza Pessoa, Felipe Arley Costa Medeiros, Jansen Fernandes Camargo, Luís Marcelo Aranha |
author_facet | Teles, Carolina Bioni Garcia dos Santos, Ana Paula de Azevedo Freitas, Rui Alves de Oliveira, Arley Faria José Ogawa, Guilherme Maerschner Rodrigues, Moreno Souza Pessoa, Felipe Arley Costa Medeiros, Jansen Fernandes Camargo, Luís Marcelo Aranha |
author_sort | Teles, Carolina Bioni Garcia |
collection | PubMed |
description | In this study, we identified the phlebotomine sandfly vectors involved in the transmission of American Cutaneous Leishmaniasis (ACL) in Assis Brasil, Acre, Brazil, which is located on the Brazil-Peru-Bolivia frontier. The genotyping of Leishmania in phlebotomines was performed using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. A total of 6,850 sandflies comprising 67 species were captured by using CDC light traps in rural areas of the municipality. Three sandfly species were found in the state of Acre for the first time: Lutzomyia georgii, Lu. complexa and Lu. evangelistai. The predominant species was Lu. auraensis/Lu. ruifreitasi and Lu. davisi (total 59.27%). 32 of 368 pools were positive for the presence of Leishmania DNA (16 pools corresponding to Lu. davisi, and 16 corresponding to Lu. auraensis/Lu. ruifreitasi), with a minimal infection prevalence of 1.85% in Lu. davisi and 2.05% in Lu. auraensis/Lu. ruifreitasi. The Leishmania species found showed maximum identity with L. (Viannia) guyanensis and L. (V.) braziliensis in both phlebotomine species. Based on these results and similar scenarios previously described along the Brazil/Peru/Bolivia tri-border, the studied area must take into consideration the possibility of Lu. davisi and Lu. auraensis/Lu. ruifreitasi as probable vectors of ACL in this municipality. |
format | Online Article Text |
id | pubmed-4957494 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Instituto Oswaldo Cruz, Ministério da Saúde |
record_format | MEDLINE/PubMed |
spelling | pubmed-49574942016-07-27 Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia Teles, Carolina Bioni Garcia dos Santos, Ana Paula de Azevedo Freitas, Rui Alves de Oliveira, Arley Faria José Ogawa, Guilherme Maerschner Rodrigues, Moreno Souza Pessoa, Felipe Arley Costa Medeiros, Jansen Fernandes Camargo, Luís Marcelo Aranha Mem Inst Oswaldo Cruz Articles In this study, we identified the phlebotomine sandfly vectors involved in the transmission of American Cutaneous Leishmaniasis (ACL) in Assis Brasil, Acre, Brazil, which is located on the Brazil-Peru-Bolivia frontier. The genotyping of Leishmania in phlebotomines was performed using polymerase chain reaction (PCR) and PCR-restriction fragment length polymorphism. A total of 6,850 sandflies comprising 67 species were captured by using CDC light traps in rural areas of the municipality. Three sandfly species were found in the state of Acre for the first time: Lutzomyia georgii, Lu. complexa and Lu. evangelistai. The predominant species was Lu. auraensis/Lu. ruifreitasi and Lu. davisi (total 59.27%). 32 of 368 pools were positive for the presence of Leishmania DNA (16 pools corresponding to Lu. davisi, and 16 corresponding to Lu. auraensis/Lu. ruifreitasi), with a minimal infection prevalence of 1.85% in Lu. davisi and 2.05% in Lu. auraensis/Lu. ruifreitasi. The Leishmania species found showed maximum identity with L. (Viannia) guyanensis and L. (V.) braziliensis in both phlebotomine species. Based on these results and similar scenarios previously described along the Brazil/Peru/Bolivia tri-border, the studied area must take into consideration the possibility of Lu. davisi and Lu. auraensis/Lu. ruifreitasi as probable vectors of ACL in this municipality. Instituto Oswaldo Cruz, Ministério da Saúde 2016-06-10 2016-07 /pmc/articles/PMC4957494/ /pubmed/27304023 http://dx.doi.org/10.1590/0074-02760160054 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Teles, Carolina Bioni Garcia dos Santos, Ana Paula de Azevedo Freitas, Rui Alves de Oliveira, Arley Faria José Ogawa, Guilherme Maerschner Rodrigues, Moreno Souza Pessoa, Felipe Arley Costa Medeiros, Jansen Fernandes Camargo, Luís Marcelo Aranha Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
title | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
title_full | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
title_fullStr | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
title_full_unstemmed | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
title_short | Phlebotomine sandfly (Diptera: Psychodidae) diversity and their Leishmania DNA in a hot spot of American Cutaneous Leishmaniasis human cases along the Brazilian border with Peru and Bolivia |
title_sort | phlebotomine sandfly (diptera: psychodidae) diversity and their leishmania dna in a hot spot of american cutaneous leishmaniasis human cases along the brazilian border with peru and bolivia |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957494/ https://www.ncbi.nlm.nih.gov/pubmed/27304023 http://dx.doi.org/10.1590/0074-02760160054 |
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