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Aurora-A regulates MCRS1 function during mitosis
The mitotic spindle is made of microtubules (MTs) nucleated through different pathways involving the centrosomes, the chromosomes or the walls of pre-existing MTs. MCRS1 is a RanGTP target that specifically associates with the chromosome-driven MTs protecting them from MT depolymerases. MCRS1 is als...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957591/ https://www.ncbi.nlm.nih.gov/pubmed/27192185 http://dx.doi.org/10.1080/15384101.2016.1187342 |
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author | Meunier, Sylvain Timón, Krystal Vernos, Isabelle |
author_facet | Meunier, Sylvain Timón, Krystal Vernos, Isabelle |
author_sort | Meunier, Sylvain |
collection | PubMed |
description | The mitotic spindle is made of microtubules (MTs) nucleated through different pathways involving the centrosomes, the chromosomes or the walls of pre-existing MTs. MCRS1 is a RanGTP target that specifically associates with the chromosome-driven MTs protecting them from MT depolymerases. MCRS1 is also needed for the control of kinetochore fiber (K-fiber) MT minus-ends dynamics in metaphase. Here, we investigated the regulation of MCRS1 activity in M-phase. We show that MCRS1 is phosphorylated by the Aurora-A kinase in mitosis on Ser35/36. Although this phosphorylation has no role on MCRS1 localization to chromosomal MTs and K-fiber minus-ends, we show that it regulates MCRS1 activity in mitosis. We conclude that Aurora-A activity is particularly important in the tuning of K-fiber minus-ends dynamics in mitosis. |
format | Online Article Text |
id | pubmed-4957591 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-49575912016-08-05 Aurora-A regulates MCRS1 function during mitosis Meunier, Sylvain Timón, Krystal Vernos, Isabelle Cell Cycle Reports The mitotic spindle is made of microtubules (MTs) nucleated through different pathways involving the centrosomes, the chromosomes or the walls of pre-existing MTs. MCRS1 is a RanGTP target that specifically associates with the chromosome-driven MTs protecting them from MT depolymerases. MCRS1 is also needed for the control of kinetochore fiber (K-fiber) MT minus-ends dynamics in metaphase. Here, we investigated the regulation of MCRS1 activity in M-phase. We show that MCRS1 is phosphorylated by the Aurora-A kinase in mitosis on Ser35/36. Although this phosphorylation has no role on MCRS1 localization to chromosomal MTs and K-fiber minus-ends, we show that it regulates MCRS1 activity in mitosis. We conclude that Aurora-A activity is particularly important in the tuning of K-fiber minus-ends dynamics in mitosis. Taylor & Francis 2016-05-18 /pmc/articles/PMC4957591/ /pubmed/27192185 http://dx.doi.org/10.1080/15384101.2016.1187342 Text en © 2016 The Author(s). Published with license by Taylor & Francis. http://creativecommons.org/licenses/by-nc/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. The moral rights of the named author(s) have been asserted. |
spellingShingle | Reports Meunier, Sylvain Timón, Krystal Vernos, Isabelle Aurora-A regulates MCRS1 function during mitosis |
title | Aurora-A regulates MCRS1 function during mitosis |
title_full | Aurora-A regulates MCRS1 function during mitosis |
title_fullStr | Aurora-A regulates MCRS1 function during mitosis |
title_full_unstemmed | Aurora-A regulates MCRS1 function during mitosis |
title_short | Aurora-A regulates MCRS1 function during mitosis |
title_sort | aurora-a regulates mcrs1 function during mitosis |
topic | Reports |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957591/ https://www.ncbi.nlm.nih.gov/pubmed/27192185 http://dx.doi.org/10.1080/15384101.2016.1187342 |
work_keys_str_mv | AT meuniersylvain auroraaregulatesmcrs1functionduringmitosis AT timonkrystal auroraaregulatesmcrs1functionduringmitosis AT vernosisabelle auroraaregulatesmcrs1functionduringmitosis |