Cargando…

Evaluation of the NanoCHIP(®) Gastrointestinal Panel (GIP) Test for Simultaneous Detection of Parasitic and Bacterial Enteric Pathogens in Fecal Specimens

Infectious gastroenteritis is a global health problem associated with high morbidity and mortality rates. Rapid and accurate diagnosis is crucial to allow appropriate and timely treatment. Current laboratory stool testing has a long turnaround time (TAT) and demands highly qualified personnel and mu...

Descripción completa

Detalles Bibliográficos
Autores principales: Ken Dror, Shifra, Pavlotzky, Elsa, Barak, Mira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957780/
https://www.ncbi.nlm.nih.gov/pubmed/27447173
http://dx.doi.org/10.1371/journal.pone.0159440
_version_ 1782444224067665920
author Ken Dror, Shifra
Pavlotzky, Elsa
Barak, Mira
author_facet Ken Dror, Shifra
Pavlotzky, Elsa
Barak, Mira
author_sort Ken Dror, Shifra
collection PubMed
description Infectious gastroenteritis is a global health problem associated with high morbidity and mortality rates. Rapid and accurate diagnosis is crucial to allow appropriate and timely treatment. Current laboratory stool testing has a long turnaround time (TAT) and demands highly qualified personnel and multiple techniques. The need for high throughput and the number of possible enteric pathogens compels the implementation of a molecular approach which uses multiplex technology, without compromising performance requirements. In this work we evaluated the feasibility of the NanoCHIP(®) Gastrointestinal Panel (GIP) (Savyon Diagnostics, Ashdod, IL), a molecular microarray-based screening test, to be used in the routine workflow of our laboratory, a big outpatient microbiology laboratory. The NanoCHIP(®) GIP test provides simultaneous detection of nine major enteric bacteria and parasites: Campylobacter spp., Salmonella spp., Shigella spp., Giardia sp., Cryptosporidium spp., Entamoeba histolytica, Entamoeba dispar, Dientamoeba fragilis, and Blastocystis spp. The required high-throughput was obtained by the NanoCHIP(®) detection system together with the MagNA Pure 96 DNA purification system (Roche Diagnostics Ltd., Switzerland). This combined system has demonstrated a higher sensitivity and detection yield compared to the conventional methods in both, retrospective and prospective samples. The identification of multiple parasites and bacteria in a single test also enabled increased efficiency of detecting mixed infections, as well as reduced hands-on time and work load. In conclusion, the combination of these two automated systems is a proper response to the laboratory needs in terms of improving laboratory workflow, turn-around-time, minimizing human errors and can be efficiently integrated in the routine work of the laboratory.
format Online
Article
Text
id pubmed-4957780
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-49577802016-08-08 Evaluation of the NanoCHIP(®) Gastrointestinal Panel (GIP) Test for Simultaneous Detection of Parasitic and Bacterial Enteric Pathogens in Fecal Specimens Ken Dror, Shifra Pavlotzky, Elsa Barak, Mira PLoS One Research Article Infectious gastroenteritis is a global health problem associated with high morbidity and mortality rates. Rapid and accurate diagnosis is crucial to allow appropriate and timely treatment. Current laboratory stool testing has a long turnaround time (TAT) and demands highly qualified personnel and multiple techniques. The need for high throughput and the number of possible enteric pathogens compels the implementation of a molecular approach which uses multiplex technology, without compromising performance requirements. In this work we evaluated the feasibility of the NanoCHIP(®) Gastrointestinal Panel (GIP) (Savyon Diagnostics, Ashdod, IL), a molecular microarray-based screening test, to be used in the routine workflow of our laboratory, a big outpatient microbiology laboratory. The NanoCHIP(®) GIP test provides simultaneous detection of nine major enteric bacteria and parasites: Campylobacter spp., Salmonella spp., Shigella spp., Giardia sp., Cryptosporidium spp., Entamoeba histolytica, Entamoeba dispar, Dientamoeba fragilis, and Blastocystis spp. The required high-throughput was obtained by the NanoCHIP(®) detection system together with the MagNA Pure 96 DNA purification system (Roche Diagnostics Ltd., Switzerland). This combined system has demonstrated a higher sensitivity and detection yield compared to the conventional methods in both, retrospective and prospective samples. The identification of multiple parasites and bacteria in a single test also enabled increased efficiency of detecting mixed infections, as well as reduced hands-on time and work load. In conclusion, the combination of these two automated systems is a proper response to the laboratory needs in terms of improving laboratory workflow, turn-around-time, minimizing human errors and can be efficiently integrated in the routine work of the laboratory. Public Library of Science 2016-07-22 /pmc/articles/PMC4957780/ /pubmed/27447173 http://dx.doi.org/10.1371/journal.pone.0159440 Text en © 2016 Ken Dror et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Ken Dror, Shifra
Pavlotzky, Elsa
Barak, Mira
Evaluation of the NanoCHIP(®) Gastrointestinal Panel (GIP) Test for Simultaneous Detection of Parasitic and Bacterial Enteric Pathogens in Fecal Specimens
title Evaluation of the NanoCHIP(®) Gastrointestinal Panel (GIP) Test for Simultaneous Detection of Parasitic and Bacterial Enteric Pathogens in Fecal Specimens
title_full Evaluation of the NanoCHIP(®) Gastrointestinal Panel (GIP) Test for Simultaneous Detection of Parasitic and Bacterial Enteric Pathogens in Fecal Specimens
title_fullStr Evaluation of the NanoCHIP(®) Gastrointestinal Panel (GIP) Test for Simultaneous Detection of Parasitic and Bacterial Enteric Pathogens in Fecal Specimens
title_full_unstemmed Evaluation of the NanoCHIP(®) Gastrointestinal Panel (GIP) Test for Simultaneous Detection of Parasitic and Bacterial Enteric Pathogens in Fecal Specimens
title_short Evaluation of the NanoCHIP(®) Gastrointestinal Panel (GIP) Test for Simultaneous Detection of Parasitic and Bacterial Enteric Pathogens in Fecal Specimens
title_sort evaluation of the nanochip(®) gastrointestinal panel (gip) test for simultaneous detection of parasitic and bacterial enteric pathogens in fecal specimens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957780/
https://www.ncbi.nlm.nih.gov/pubmed/27447173
http://dx.doi.org/10.1371/journal.pone.0159440
work_keys_str_mv AT kendrorshifra evaluationofthenanochipgastrointestinalpanelgiptestforsimultaneousdetectionofparasiticandbacterialentericpathogensinfecalspecimens
AT pavlotzkyelsa evaluationofthenanochipgastrointestinalpanelgiptestforsimultaneousdetectionofparasiticandbacterialentericpathogensinfecalspecimens
AT barakmira evaluationofthenanochipgastrointestinalpanelgiptestforsimultaneousdetectionofparasiticandbacterialentericpathogensinfecalspecimens