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Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV

BACKGROUND: Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previ...

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Autores principales: Oellers, Dana E., Bauer, Natali, Ginder, Melanie, Johannes, Sigrid, Pernecker, Iris, Moritz, Andreas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957853/
https://www.ncbi.nlm.nih.gov/pubmed/27449509
http://dx.doi.org/10.1186/s12917-016-0779-4
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author Oellers, Dana E.
Bauer, Natali
Ginder, Melanie
Johannes, Sigrid
Pernecker, Iris
Moritz, Andreas
author_facet Oellers, Dana E.
Bauer, Natali
Ginder, Melanie
Johannes, Sigrid
Pernecker, Iris
Moritz, Andreas
author_sort Oellers, Dana E.
collection PubMed
description BACKGROUND: Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previously (Pankraz et al., Vet Clin Path 38:30–38, 2009; Gelain et al., High fluorescent platelets fraction in macrothrombocytopenic Norfolk terrier, 2010) is largely unknown. The aim was to compare different published gates for measurement of r-PLTs with the Sysmex XT-2000iV with an own, optimized gate (“Oellers-gate”) and to establish reference intervals (RIs) in > 120 dogs. Data of 362 measurements of diseased and healthy dogs were analyzed retrospectively. Several gates were applied and RIs for r-PLTs and platelet indices were established for pet dogs and a group of 153 healthy Beagles kept under defined housing conditions. Intra-assay precision (CV) was also assessed. RESULTS: In 30/362 samples, interferences consistent with small erythrocytes/reticulocytes were seen in the previously published gates but not in the “Oellers-gate”. Good correlation was found between the different gates (r(s): 0.88–1.00). RIs for the “Pankraz-gate”, the “Gelain-gate”, and the “Oellers-gate” were 0.0–1.2, 0.2–3.7 and 0.2–3.9 % respectively. CVs were ranging between 22 and 41 %. CONCLUSIONS: Optimization of previously published gates minimized interferences of small erythrocytes with r-PLT measurements.
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spelling pubmed-49578532016-07-23 Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV Oellers, Dana E. Bauer, Natali Ginder, Melanie Johannes, Sigrid Pernecker, Iris Moritz, Andreas BMC Vet Res Methodology Article BACKGROUND: Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previously (Pankraz et al., Vet Clin Path 38:30–38, 2009; Gelain et al., High fluorescent platelets fraction in macrothrombocytopenic Norfolk terrier, 2010) is largely unknown. The aim was to compare different published gates for measurement of r-PLTs with the Sysmex XT-2000iV with an own, optimized gate (“Oellers-gate”) and to establish reference intervals (RIs) in > 120 dogs. Data of 362 measurements of diseased and healthy dogs were analyzed retrospectively. Several gates were applied and RIs for r-PLTs and platelet indices were established for pet dogs and a group of 153 healthy Beagles kept under defined housing conditions. Intra-assay precision (CV) was also assessed. RESULTS: In 30/362 samples, interferences consistent with small erythrocytes/reticulocytes were seen in the previously published gates but not in the “Oellers-gate”. Good correlation was found between the different gates (r(s): 0.88–1.00). RIs for the “Pankraz-gate”, the “Gelain-gate”, and the “Oellers-gate” were 0.0–1.2, 0.2–3.7 and 0.2–3.9 % respectively. CVs were ranging between 22 and 41 %. CONCLUSIONS: Optimization of previously published gates minimized interferences of small erythrocytes with r-PLT measurements. BioMed Central 2016-07-22 /pmc/articles/PMC4957853/ /pubmed/27449509 http://dx.doi.org/10.1186/s12917-016-0779-4 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Methodology Article
Oellers, Dana E.
Bauer, Natali
Ginder, Melanie
Johannes, Sigrid
Pernecker, Iris
Moritz, Andreas
Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV
title Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV
title_full Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV
title_fullStr Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV
title_full_unstemmed Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV
title_short Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV
title_sort optimized gating and reference ranges of reticulated platelets in dogs for the sysmex xt-2000iv
topic Methodology Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957853/
https://www.ncbi.nlm.nih.gov/pubmed/27449509
http://dx.doi.org/10.1186/s12917-016-0779-4
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