Cargando…
Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV
BACKGROUND: Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previ...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957853/ https://www.ncbi.nlm.nih.gov/pubmed/27449509 http://dx.doi.org/10.1186/s12917-016-0779-4 |
_version_ | 1782444237590102016 |
---|---|
author | Oellers, Dana E. Bauer, Natali Ginder, Melanie Johannes, Sigrid Pernecker, Iris Moritz, Andreas |
author_facet | Oellers, Dana E. Bauer, Natali Ginder, Melanie Johannes, Sigrid Pernecker, Iris Moritz, Andreas |
author_sort | Oellers, Dana E. |
collection | PubMed |
description | BACKGROUND: Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previously (Pankraz et al., Vet Clin Path 38:30–38, 2009; Gelain et al., High fluorescent platelets fraction in macrothrombocytopenic Norfolk terrier, 2010) is largely unknown. The aim was to compare different published gates for measurement of r-PLTs with the Sysmex XT-2000iV with an own, optimized gate (“Oellers-gate”) and to establish reference intervals (RIs) in > 120 dogs. Data of 362 measurements of diseased and healthy dogs were analyzed retrospectively. Several gates were applied and RIs for r-PLTs and platelet indices were established for pet dogs and a group of 153 healthy Beagles kept under defined housing conditions. Intra-assay precision (CV) was also assessed. RESULTS: In 30/362 samples, interferences consistent with small erythrocytes/reticulocytes were seen in the previously published gates but not in the “Oellers-gate”. Good correlation was found between the different gates (r(s): 0.88–1.00). RIs for the “Pankraz-gate”, the “Gelain-gate”, and the “Oellers-gate” were 0.0–1.2, 0.2–3.7 and 0.2–3.9 % respectively. CVs were ranging between 22 and 41 %. CONCLUSIONS: Optimization of previously published gates minimized interferences of small erythrocytes with r-PLT measurements. |
format | Online Article Text |
id | pubmed-4957853 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49578532016-07-23 Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV Oellers, Dana E. Bauer, Natali Ginder, Melanie Johannes, Sigrid Pernecker, Iris Moritz, Andreas BMC Vet Res Methodology Article BACKGROUND: Canine reticulated platelets (r-PLTs) i.e., juvenile PLTs reflecting thrombopoiesis can be measured automatically with the hematology analyzer Sysmex XT-2000iV using manual gating options. However, the impact of interferences on r-PLT measurements performed with the gates published previously (Pankraz et al., Vet Clin Path 38:30–38, 2009; Gelain et al., High fluorescent platelets fraction in macrothrombocytopenic Norfolk terrier, 2010) is largely unknown. The aim was to compare different published gates for measurement of r-PLTs with the Sysmex XT-2000iV with an own, optimized gate (“Oellers-gate”) and to establish reference intervals (RIs) in > 120 dogs. Data of 362 measurements of diseased and healthy dogs were analyzed retrospectively. Several gates were applied and RIs for r-PLTs and platelet indices were established for pet dogs and a group of 153 healthy Beagles kept under defined housing conditions. Intra-assay precision (CV) was also assessed. RESULTS: In 30/362 samples, interferences consistent with small erythrocytes/reticulocytes were seen in the previously published gates but not in the “Oellers-gate”. Good correlation was found between the different gates (r(s): 0.88–1.00). RIs for the “Pankraz-gate”, the “Gelain-gate”, and the “Oellers-gate” were 0.0–1.2, 0.2–3.7 and 0.2–3.9 % respectively. CVs were ranging between 22 and 41 %. CONCLUSIONS: Optimization of previously published gates minimized interferences of small erythrocytes with r-PLT measurements. BioMed Central 2016-07-22 /pmc/articles/PMC4957853/ /pubmed/27449509 http://dx.doi.org/10.1186/s12917-016-0779-4 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Methodology Article Oellers, Dana E. Bauer, Natali Ginder, Melanie Johannes, Sigrid Pernecker, Iris Moritz, Andreas Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV |
title | Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV |
title_full | Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV |
title_fullStr | Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV |
title_full_unstemmed | Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV |
title_short | Optimized gating and reference ranges of reticulated platelets in dogs for the Sysmex XT-2000iV |
title_sort | optimized gating and reference ranges of reticulated platelets in dogs for the sysmex xt-2000iv |
topic | Methodology Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4957853/ https://www.ncbi.nlm.nih.gov/pubmed/27449509 http://dx.doi.org/10.1186/s12917-016-0779-4 |
work_keys_str_mv | AT oellersdanae optimizedgatingandreferencerangesofreticulatedplateletsindogsforthesysmexxt2000iv AT bauernatali optimizedgatingandreferencerangesofreticulatedplateletsindogsforthesysmexxt2000iv AT gindermelanie optimizedgatingandreferencerangesofreticulatedplateletsindogsforthesysmexxt2000iv AT johannessigrid optimizedgatingandreferencerangesofreticulatedplateletsindogsforthesysmexxt2000iv AT perneckeriris optimizedgatingandreferencerangesofreticulatedplateletsindogsforthesysmexxt2000iv AT moritzandreas optimizedgatingandreferencerangesofreticulatedplateletsindogsforthesysmexxt2000iv |