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Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip
BACKGROUND: HerboChip is an array of different fractions deriving from herbal extracts. This study aimed to identify effective components from Chinese medicine (CM) that interact with nerve growth factor (NGF) as a target using HerboChip. METHODS: Fifty types of CM that are traditionally used as rem...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4958286/ https://www.ncbi.nlm.nih.gov/pubmed/27453720 http://dx.doi.org/10.1186/s13020-016-0107-8 |
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author | Lee, Pinky Sum Chi Zhang, Laura Minglu Yan, Artemis Lu Lam, Kelly Yin Ching Dong, Tina Tingxia Lin, Huangquan Chan, Gallant Kar Lun Tsim, Karl Wah Keung |
author_facet | Lee, Pinky Sum Chi Zhang, Laura Minglu Yan, Artemis Lu Lam, Kelly Yin Ching Dong, Tina Tingxia Lin, Huangquan Chan, Gallant Kar Lun Tsim, Karl Wah Keung |
author_sort | Lee, Pinky Sum Chi |
collection | PubMed |
description | BACKGROUND: HerboChip is an array of different fractions deriving from herbal extracts. This study aimed to identify effective components from Chinese medicine (CM) that interact with nerve growth factor (NGF) as a target using HerboChip. METHODS: Fifty types of CM that are traditionally used as remedies for emotion imbalance were selected and extracted with 50 % ethanol. Biotinylated-NGF was hybridized with over 300 chips coated with different HPLC-separated fractions from CM extracts and straptavidin-Cy5 was used to identify the NGF-bound fractions. RESULTS: Over 300 chips were screened within a week, and 17 positive hits were identified. The interaction of the identified herbal extracts with NGF was confirmed in cultured PC12 cells. Co-application of NGF and herbal extract interfered with NGF-induced expression of neurofilaments, including NF68 and NF200 in cell cultures. Western blot analysis comparing the intensity of phosphorylated cAMP response element-binding protein (CREB) over total CREB showed NGF-induced CREB phosphorylation was modulated by the identified herbal extracts. Five CM herbs showed activating activities on the NGF response and nine CM herbs showed inhibiting activities. CONCLUSION: The current result supported the applicability of HerboChip for screening NGF binding components from herbal extracts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13020-016-0107-8) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4958286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49582862016-07-24 Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip Lee, Pinky Sum Chi Zhang, Laura Minglu Yan, Artemis Lu Lam, Kelly Yin Ching Dong, Tina Tingxia Lin, Huangquan Chan, Gallant Kar Lun Tsim, Karl Wah Keung Chin Med Research BACKGROUND: HerboChip is an array of different fractions deriving from herbal extracts. This study aimed to identify effective components from Chinese medicine (CM) that interact with nerve growth factor (NGF) as a target using HerboChip. METHODS: Fifty types of CM that are traditionally used as remedies for emotion imbalance were selected and extracted with 50 % ethanol. Biotinylated-NGF was hybridized with over 300 chips coated with different HPLC-separated fractions from CM extracts and straptavidin-Cy5 was used to identify the NGF-bound fractions. RESULTS: Over 300 chips were screened within a week, and 17 positive hits were identified. The interaction of the identified herbal extracts with NGF was confirmed in cultured PC12 cells. Co-application of NGF and herbal extract interfered with NGF-induced expression of neurofilaments, including NF68 and NF200 in cell cultures. Western blot analysis comparing the intensity of phosphorylated cAMP response element-binding protein (CREB) over total CREB showed NGF-induced CREB phosphorylation was modulated by the identified herbal extracts. Five CM herbs showed activating activities on the NGF response and nine CM herbs showed inhibiting activities. CONCLUSION: The current result supported the applicability of HerboChip for screening NGF binding components from herbal extracts. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s13020-016-0107-8) contains supplementary material, which is available to authorized users. BioMed Central 2016-07-23 /pmc/articles/PMC4958286/ /pubmed/27453720 http://dx.doi.org/10.1186/s13020-016-0107-8 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Lee, Pinky Sum Chi Zhang, Laura Minglu Yan, Artemis Lu Lam, Kelly Yin Ching Dong, Tina Tingxia Lin, Huangquan Chan, Gallant Kar Lun Tsim, Karl Wah Keung Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip |
title | Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip |
title_full | Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip |
title_fullStr | Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip |
title_full_unstemmed | Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip |
title_short | Indication of nerve growth factor binding components from herbal extracts by HerboChip: a platform for drug screening on a chip |
title_sort | indication of nerve growth factor binding components from herbal extracts by herbochip: a platform for drug screening on a chip |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4958286/ https://www.ncbi.nlm.nih.gov/pubmed/27453720 http://dx.doi.org/10.1186/s13020-016-0107-8 |
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