A simple Gateway-assisted construction system of TALEN genes for plant genome editing

TALEN is an artificial nuclease being applied for sequence-specific genome editing. For the plant genome editing, a pair of TALEN genes is expressed in the cells, and a binary plasmid for Agrobacterium-mediated transformation should be assembled. We developed a novel procedure using the Gateway-assi...

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Detalles Bibliográficos
Autores principales: Kusano, Hiroaki, Onodera, Hitomi, Kihira, Miho, Aoki, Hiromi, Matsuzaki, Hikaru, Shimada, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4958979/
https://www.ncbi.nlm.nih.gov/pubmed/27452606
http://dx.doi.org/10.1038/srep30234

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