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Methyl group donors abrogate adaptive responses to dietary restriction in C. elegans
BACKGROUND: Almost all animals adapt to dietary restriction through alternative life history traits that affect their growth, reproduction, and survival. Economized management of fat stores is a prevalent type of such adaptations. Because one-carbon metabolism is a critical gauge of food availabilit...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959552/ https://www.ncbi.nlm.nih.gov/pubmed/27482296 http://dx.doi.org/10.1186/s12263-016-0522-4 |
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author | Klapper, Maja Findeis, Daniel Koefeler, Harald Döring, Frank |
author_facet | Klapper, Maja Findeis, Daniel Koefeler, Harald Döring, Frank |
author_sort | Klapper, Maja |
collection | PubMed |
description | BACKGROUND: Almost all animals adapt to dietary restriction through alternative life history traits that affect their growth, reproduction, and survival. Economized management of fat stores is a prevalent type of such adaptations. Because one-carbon metabolism is a critical gauge of food availability, in this study, we used Caenorhabditis elegans to test whether the methyl group donor choline regulates adaptive responses to dietary restriction. We used a modest dietary restriction regimen that prolonged the fecund period without reducing the lifetime production of progeny, which is the best measure of fitness. RESULTS: We found that dietary supplementation with choline abrogate the dietary restriction-induced prolongation of the reproductive period as well as the accumulation and delayed depletion of large lipid droplets and whole-fat stores and increased the survival rate in the cold. By contrast, the life span-prolonging effect of dietary restriction is not affected by choline. Moreover, we found that dietary restriction led to the enlargement of lipid droplets within embryos and enhancement of the cold tolerance of the progeny of dietarily restricted mothers. Both of these transgenerational responses to maternal dietary restriction were abrogated by exposing the parental generation to choline. CONCLUSIONS: In conclusion, supplementation with the methyl group donor choline abrogates distinct responses to dietary restriction related to reproduction, utilization of fat stored in large lipid droplets, cold tolerance, and thrifty phenotypes in C. elegans. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12263-016-0522-4) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4959552 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49595522016-08-01 Methyl group donors abrogate adaptive responses to dietary restriction in C. elegans Klapper, Maja Findeis, Daniel Koefeler, Harald Döring, Frank Genes Nutr Research BACKGROUND: Almost all animals adapt to dietary restriction through alternative life history traits that affect their growth, reproduction, and survival. Economized management of fat stores is a prevalent type of such adaptations. Because one-carbon metabolism is a critical gauge of food availability, in this study, we used Caenorhabditis elegans to test whether the methyl group donor choline regulates adaptive responses to dietary restriction. We used a modest dietary restriction regimen that prolonged the fecund period without reducing the lifetime production of progeny, which is the best measure of fitness. RESULTS: We found that dietary supplementation with choline abrogate the dietary restriction-induced prolongation of the reproductive period as well as the accumulation and delayed depletion of large lipid droplets and whole-fat stores and increased the survival rate in the cold. By contrast, the life span-prolonging effect of dietary restriction is not affected by choline. Moreover, we found that dietary restriction led to the enlargement of lipid droplets within embryos and enhancement of the cold tolerance of the progeny of dietarily restricted mothers. Both of these transgenerational responses to maternal dietary restriction were abrogated by exposing the parental generation to choline. CONCLUSIONS: In conclusion, supplementation with the methyl group donor choline abrogates distinct responses to dietary restriction related to reproduction, utilization of fat stored in large lipid droplets, cold tolerance, and thrifty phenotypes in C. elegans. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1186/s12263-016-0522-4) contains supplementary material, which is available to authorized users. BioMed Central 2016-03-17 /pmc/articles/PMC4959552/ /pubmed/27482296 http://dx.doi.org/10.1186/s12263-016-0522-4 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Klapper, Maja Findeis, Daniel Koefeler, Harald Döring, Frank Methyl group donors abrogate adaptive responses to dietary restriction in C. elegans |
title | Methyl group donors abrogate adaptive responses to dietary restriction in C. elegans |
title_full | Methyl group donors abrogate adaptive responses to dietary restriction in C. elegans |
title_fullStr | Methyl group donors abrogate adaptive responses to dietary restriction in C. elegans |
title_full_unstemmed | Methyl group donors abrogate adaptive responses to dietary restriction in C. elegans |
title_short | Methyl group donors abrogate adaptive responses to dietary restriction in C. elegans |
title_sort | methyl group donors abrogate adaptive responses to dietary restriction in c. elegans |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959552/ https://www.ncbi.nlm.nih.gov/pubmed/27482296 http://dx.doi.org/10.1186/s12263-016-0522-4 |
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