Programming Native CRISPR Arrays for the Generation of Targeted Immunity

The adaptive immune system of prokaryotes, called CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated genes), results in specific cleavage of invading nucleic acid sequences recognized by the cell’s “memory” of past encounters. Here, we exploited the propertie...

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Detalles Bibliográficos
Autores principales: Hynes, Alexander P., Labrie, Simon J., Moineau, Sylvain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Observation
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4959665/
https://www.ncbi.nlm.nih.gov/pubmed/27143383
http://dx.doi.org/10.1128/mBio.00202-16
Descripción
Sumario:The adaptive immune system of prokaryotes, called CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated genes), results in specific cleavage of invading nucleic acid sequences recognized by the cell’s “memory” of past encounters. Here, we exploited the properties of native CRISPR-Cas systems to program the natural “memorization” process, efficiently generating immunity not only to a bacteriophage or plasmid but to any specifically chosen DNA sequence.

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