Cargando…

Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs

Understanding the origin and differentiation mechanism of coronary vascular smooth muscle cells (CoSMCs) is very important to cardiovascular biology. The early cardiovascular system is formed in a hypoxic microenvironment, and Tbx18-positive epicardial cells are a source of CoSMCs. However, the effe...

Descripción completa

Detalles Bibliográficos
Autores principales: Jing, Xiaodong, Gao, Yulin, Xiao, Songlin, Qin, Qin, Wei, Xiaoming, Yan, Yuling, Wu, Ling, Deng, Songbai, Du, Jianlin, Liu, Yajie, She, Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4960593/
https://www.ncbi.nlm.nih.gov/pubmed/27456656
http://dx.doi.org/10.1038/srep30468
_version_ 1782444551327186944
author Jing, Xiaodong
Gao, Yulin
Xiao, Songlin
Qin, Qin
Wei, Xiaoming
Yan, Yuling
Wu, Ling
Deng, Songbai
Du, Jianlin
Liu, Yajie
She, Qiang
author_facet Jing, Xiaodong
Gao, Yulin
Xiao, Songlin
Qin, Qin
Wei, Xiaoming
Yan, Yuling
Wu, Ling
Deng, Songbai
Du, Jianlin
Liu, Yajie
She, Qiang
author_sort Jing, Xiaodong
collection PubMed
description Understanding the origin and differentiation mechanism of coronary vascular smooth muscle cells (CoSMCs) is very important to cardiovascular biology. The early cardiovascular system is formed in a hypoxic microenvironment, and Tbx18-positive epicardial cells are a source of CoSMCs. However, the effects of hypoxia on the differentiation of Tbx18-positive epicardial cells to CoSMCs and the primary regulatory mechanism are insufficiently understood. Using Tbx18:Cre/R26R(EYFP/LacZ) fate-tracing mice, we cultured highly purified Tbx18-positive epicardial cells. We further showed that hypoxia induced Tbx18-positive epicardial cells to differentiate into CoSMCs and promoted the epithelial-mesenchymal transition (EMT) process of the cells in vitro. The induction of differentiation was primarily achieved via the hypoxia inducible factor-1α (HIF-1α)-mediated effects exerted on Snail. Using a cell migration assay, we showed that hypoxia enhanced the motility of Tbx18-positive epicardial cells. By constructing a hypoxic model of the embryonic epicardium in vivo, we showed that hypoxia led to premature in situ differentiation of Tbx18-positive epicardial cells to CoSMCs. Furthermore, hypoxia was sufficient to induce Snail expression in Tbx18-positive epicardial cells in vivo. Our study suggests that hypoxia intervention was sufficient to induce the differentiation of Tbx18-positive epicardial cells to CoSMCs. Furthermore, this differentiation was achieved primarily via HIF-1α-mediated regulation of Snail.
format Online
Article
Text
id pubmed-4960593
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-49605932016-08-05 Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs Jing, Xiaodong Gao, Yulin Xiao, Songlin Qin, Qin Wei, Xiaoming Yan, Yuling Wu, Ling Deng, Songbai Du, Jianlin Liu, Yajie She, Qiang Sci Rep Article Understanding the origin and differentiation mechanism of coronary vascular smooth muscle cells (CoSMCs) is very important to cardiovascular biology. The early cardiovascular system is formed in a hypoxic microenvironment, and Tbx18-positive epicardial cells are a source of CoSMCs. However, the effects of hypoxia on the differentiation of Tbx18-positive epicardial cells to CoSMCs and the primary regulatory mechanism are insufficiently understood. Using Tbx18:Cre/R26R(EYFP/LacZ) fate-tracing mice, we cultured highly purified Tbx18-positive epicardial cells. We further showed that hypoxia induced Tbx18-positive epicardial cells to differentiate into CoSMCs and promoted the epithelial-mesenchymal transition (EMT) process of the cells in vitro. The induction of differentiation was primarily achieved via the hypoxia inducible factor-1α (HIF-1α)-mediated effects exerted on Snail. Using a cell migration assay, we showed that hypoxia enhanced the motility of Tbx18-positive epicardial cells. By constructing a hypoxic model of the embryonic epicardium in vivo, we showed that hypoxia led to premature in situ differentiation of Tbx18-positive epicardial cells to CoSMCs. Furthermore, hypoxia was sufficient to induce Snail expression in Tbx18-positive epicardial cells in vivo. Our study suggests that hypoxia intervention was sufficient to induce the differentiation of Tbx18-positive epicardial cells to CoSMCs. Furthermore, this differentiation was achieved primarily via HIF-1α-mediated regulation of Snail. Nature Publishing Group 2016-07-26 /pmc/articles/PMC4960593/ /pubmed/27456656 http://dx.doi.org/10.1038/srep30468 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Jing, Xiaodong
Gao, Yulin
Xiao, Songlin
Qin, Qin
Wei, Xiaoming
Yan, Yuling
Wu, Ling
Deng, Songbai
Du, Jianlin
Liu, Yajie
She, Qiang
Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs
title Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs
title_full Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs
title_fullStr Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs
title_full_unstemmed Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs
title_short Hypoxia induced the differentiation of Tbx18-positive epicardial cells to CoSMCs
title_sort hypoxia induced the differentiation of tbx18-positive epicardial cells to cosmcs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4960593/
https://www.ncbi.nlm.nih.gov/pubmed/27456656
http://dx.doi.org/10.1038/srep30468
work_keys_str_mv AT jingxiaodong hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT gaoyulin hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT xiaosonglin hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT qinqin hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT weixiaoming hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT yanyuling hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT wuling hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT dengsongbai hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT dujianlin hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT liuyajie hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs
AT sheqiang hypoxiainducedthedifferentiationoftbx18positiveepicardialcellstocosmcs