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Post-Mortem Corneal Thickness Measurements with a Portable Optical Coherence Tomography System: a Reliability Study

The purpose of this study was to determine the repeatability and reproducibility of post-mortem central corneal thickness (CCT) measurements by using a real-time, portable optical coherence tomography (OCT) system on an animal model, and to prospectively evaluate the time-course of post-mortem chang...

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Autores principales: Napoli, Pietro Emanuele, Nioi, Matteo, d’Aloja, Ernesto, Fossarello, Maurizio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4960653/
https://www.ncbi.nlm.nih.gov/pubmed/27457021
http://dx.doi.org/10.1038/srep30428
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author Napoli, Pietro Emanuele
Nioi, Matteo
d’Aloja, Ernesto
Fossarello, Maurizio
author_facet Napoli, Pietro Emanuele
Nioi, Matteo
d’Aloja, Ernesto
Fossarello, Maurizio
author_sort Napoli, Pietro Emanuele
collection PubMed
description The purpose of this study was to determine the repeatability and reproducibility of post-mortem central corneal thickness (CCT) measurements by using a real-time, portable optical coherence tomography (OCT) system on an animal model, and to prospectively evaluate the time-course of post-mortem changes in CCT. Forty-six ocular globes of sheep (Ovis aries) were analyzed with a portable spectral-domain OCT device by two operators at different postmortem intervals (PMIs) as follows: immediately (i.e. within 10 minutes), at the 30(th) minute, at the 1(st), 6(th), 12(th), 24(th) and 48(th) hour, and later (up to the 96(th) hour). The coefficient of repeatability ranged from 0.3% to 3.5%, and coefficients of reproducibility ranged from 0.2% to 3.7% in the central region of the cornea. The intraclass correlation coefficients were particularly high at different PMIs, thus confirming good measurement reliability with the portable OCT. The average CCT decreased immediately and then increased thereafter, with two peaks at 6 and 24 hours after death. Our results suggest that portable OCT is a reliable tool for monitoring CCT variations after death and may be useful in characterizing corneas before explantation, detecting quantitative variations during post-mortem corneal degeneration or assessing changes in CCT for forensic implications.
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spelling pubmed-49606532016-08-05 Post-Mortem Corneal Thickness Measurements with a Portable Optical Coherence Tomography System: a Reliability Study Napoli, Pietro Emanuele Nioi, Matteo d’Aloja, Ernesto Fossarello, Maurizio Sci Rep Article The purpose of this study was to determine the repeatability and reproducibility of post-mortem central corneal thickness (CCT) measurements by using a real-time, portable optical coherence tomography (OCT) system on an animal model, and to prospectively evaluate the time-course of post-mortem changes in CCT. Forty-six ocular globes of sheep (Ovis aries) were analyzed with a portable spectral-domain OCT device by two operators at different postmortem intervals (PMIs) as follows: immediately (i.e. within 10 minutes), at the 30(th) minute, at the 1(st), 6(th), 12(th), 24(th) and 48(th) hour, and later (up to the 96(th) hour). The coefficient of repeatability ranged from 0.3% to 3.5%, and coefficients of reproducibility ranged from 0.2% to 3.7% in the central region of the cornea. The intraclass correlation coefficients were particularly high at different PMIs, thus confirming good measurement reliability with the portable OCT. The average CCT decreased immediately and then increased thereafter, with two peaks at 6 and 24 hours after death. Our results suggest that portable OCT is a reliable tool for monitoring CCT variations after death and may be useful in characterizing corneas before explantation, detecting quantitative variations during post-mortem corneal degeneration or assessing changes in CCT for forensic implications. Nature Publishing Group 2016-07-26 /pmc/articles/PMC4960653/ /pubmed/27457021 http://dx.doi.org/10.1038/srep30428 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Napoli, Pietro Emanuele
Nioi, Matteo
d’Aloja, Ernesto
Fossarello, Maurizio
Post-Mortem Corneal Thickness Measurements with a Portable Optical Coherence Tomography System: a Reliability Study
title Post-Mortem Corneal Thickness Measurements with a Portable Optical Coherence Tomography System: a Reliability Study
title_full Post-Mortem Corneal Thickness Measurements with a Portable Optical Coherence Tomography System: a Reliability Study
title_fullStr Post-Mortem Corneal Thickness Measurements with a Portable Optical Coherence Tomography System: a Reliability Study
title_full_unstemmed Post-Mortem Corneal Thickness Measurements with a Portable Optical Coherence Tomography System: a Reliability Study
title_short Post-Mortem Corneal Thickness Measurements with a Portable Optical Coherence Tomography System: a Reliability Study
title_sort post-mortem corneal thickness measurements with a portable optical coherence tomography system: a reliability study
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4960653/
https://www.ncbi.nlm.nih.gov/pubmed/27457021
http://dx.doi.org/10.1038/srep30428
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