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16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae
Data presented here contains metagenomic analysis regarding the sequential conventionalization of germ-free zebrafish embryos. Zebrafish embryos that underwent a germ-free sterilization process immediately after fertilization were promptly exposed to and raised to larval stage in conventional fish w...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961299/ https://www.ncbi.nlm.nih.gov/pubmed/27508247 http://dx.doi.org/10.1016/j.dib.2016.06.057 |
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author | Davis, Daniel J. Bryda, Elizabeth C. Gillespie, Catherine H. Ericsson, Aaron C. |
author_facet | Davis, Daniel J. Bryda, Elizabeth C. Gillespie, Catherine H. Ericsson, Aaron C. |
author_sort | Davis, Daniel J. |
collection | PubMed |
description | Data presented here contains metagenomic analysis regarding the sequential conventionalization of germ-free zebrafish embryos. Zebrafish embryos that underwent a germ-free sterilization process immediately after fertilization were promptly exposed to and raised to larval stage in conventional fish water. At 6 days postfertilization (dpf), these “conventionalized” larvae were compared to zebrafish larvae that were raised in conventional fish water never undergoing the initial sterilization process. Bacterial 16S rRNA amplicon sequencing was performed on DNA isolated from homogenates of the larvae revealing distinct microbiota variations between the two groups. The dataset described here is also related to the research article entitled “Microbial modulation of behavior and stress responses in zebrafish larvae” (Davis et al., 2016) [1]. |
format | Online Article Text |
id | pubmed-4961299 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-49612992016-08-09 16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae Davis, Daniel J. Bryda, Elizabeth C. Gillespie, Catherine H. Ericsson, Aaron C. Data Brief Data Article Data presented here contains metagenomic analysis regarding the sequential conventionalization of germ-free zebrafish embryos. Zebrafish embryos that underwent a germ-free sterilization process immediately after fertilization were promptly exposed to and raised to larval stage in conventional fish water. At 6 days postfertilization (dpf), these “conventionalized” larvae were compared to zebrafish larvae that were raised in conventional fish water never undergoing the initial sterilization process. Bacterial 16S rRNA amplicon sequencing was performed on DNA isolated from homogenates of the larvae revealing distinct microbiota variations between the two groups. The dataset described here is also related to the research article entitled “Microbial modulation of behavior and stress responses in zebrafish larvae” (Davis et al., 2016) [1]. Elsevier 2016-07-05 /pmc/articles/PMC4961299/ /pubmed/27508247 http://dx.doi.org/10.1016/j.dib.2016.06.057 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Data Article Davis, Daniel J. Bryda, Elizabeth C. Gillespie, Catherine H. Ericsson, Aaron C. 16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae |
title | 16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae |
title_full | 16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae |
title_fullStr | 16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae |
title_full_unstemmed | 16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae |
title_short | 16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae |
title_sort | 16s rrna amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae |
topic | Data Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961299/ https://www.ncbi.nlm.nih.gov/pubmed/27508247 http://dx.doi.org/10.1016/j.dib.2016.06.057 |
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