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Estimation of Tau and Phosphorylated Tau(181) in Serum of Alzheimer’s Disease and Mild Cognitive Impairment Patients
The elevated level of cerebrospinal fluid (CSF) Tau and phosphorylated Tau(181) (p-Tau(181)) proteins are well established hallmarks of Alzheimer’s disease (AD). Elevated level of p-Tau(181) can differentiate AD from other neurodegenerative disease. However, the expression level of these proteins in...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961391/ https://www.ncbi.nlm.nih.gov/pubmed/27459603 http://dx.doi.org/10.1371/journal.pone.0159099 |
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author | Shekhar, Shashank Kumar, Rahul Rai, Nitish Kumar, Vijay Singh, Kusum Upadhyay, Ashish Datt Tripathi, Manjari Dwivedi, Sadanand Dey, Aparajit B. Dey, Sharmistha |
author_facet | Shekhar, Shashank Kumar, Rahul Rai, Nitish Kumar, Vijay Singh, Kusum Upadhyay, Ashish Datt Tripathi, Manjari Dwivedi, Sadanand Dey, Aparajit B. Dey, Sharmistha |
author_sort | Shekhar, Shashank |
collection | PubMed |
description | The elevated level of cerebrospinal fluid (CSF) Tau and phosphorylated Tau(181) (p-Tau(181)) proteins are well established hallmarks of Alzheimer’s disease (AD). Elevated level of p-Tau(181) can differentiate AD from other neurodegenerative disease. However, the expression level of these proteins in serum of AD patient is not well set up. This study sought to evaluate the level of Tau and p-Tau(181) in serum of AD, and mild cognitive impairment (MCI) patients for an alternative approach to establish protein-based markers by convenient way. Blood samples were collected from 39 AD patients, 37 MCI patients and 37 elderly individuals as controls. The levels of Tau and p-Tau(181) in the serum of the different groups were measured by label free real time Surface Plasmon Resonance technology by using specific antibodies, and were further confirmed by the conventional western blot method. An appropriate statistical analysis, including Receiver Operating Characteristic (ROC), was performed. The concentrations of serum Tau and p-Tau(181) were significantly higher (p<0.00001) in AD (Tau; 47.49±9.00ng/μL, p-Tau(181); 0.161±0.04 ng/μL) compared to MCI (Tau; 39.26±7.78 ng/μL, p-Tau(181); 0.135±0.02 ng/μL) and were further higher compared to elderly controls (Tau; 34.92±6.58 ng/μL, p-Tau(181); 0.122±0.01 ng/ μL). A significant (p<0.0001) downhill correlation was found between Tau as well as p-Tau(181) levels with HMSE and MoCA score. This study for the first time reports the concentration of Tau and p-Tau(181) in serum of AD and MCI patients. The cutoff values of Tau and p-Tau(181) of AD and MCI patients with sensitivity and specificity reveal that serum level of these proteins can be used as a predictive marker for AD and MCI. |
format | Online Article Text |
id | pubmed-4961391 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49613912016-08-08 Estimation of Tau and Phosphorylated Tau(181) in Serum of Alzheimer’s Disease and Mild Cognitive Impairment Patients Shekhar, Shashank Kumar, Rahul Rai, Nitish Kumar, Vijay Singh, Kusum Upadhyay, Ashish Datt Tripathi, Manjari Dwivedi, Sadanand Dey, Aparajit B. Dey, Sharmistha PLoS One Research Article The elevated level of cerebrospinal fluid (CSF) Tau and phosphorylated Tau(181) (p-Tau(181)) proteins are well established hallmarks of Alzheimer’s disease (AD). Elevated level of p-Tau(181) can differentiate AD from other neurodegenerative disease. However, the expression level of these proteins in serum of AD patient is not well set up. This study sought to evaluate the level of Tau and p-Tau(181) in serum of AD, and mild cognitive impairment (MCI) patients for an alternative approach to establish protein-based markers by convenient way. Blood samples were collected from 39 AD patients, 37 MCI patients and 37 elderly individuals as controls. The levels of Tau and p-Tau(181) in the serum of the different groups were measured by label free real time Surface Plasmon Resonance technology by using specific antibodies, and were further confirmed by the conventional western blot method. An appropriate statistical analysis, including Receiver Operating Characteristic (ROC), was performed. The concentrations of serum Tau and p-Tau(181) were significantly higher (p<0.00001) in AD (Tau; 47.49±9.00ng/μL, p-Tau(181); 0.161±0.04 ng/μL) compared to MCI (Tau; 39.26±7.78 ng/μL, p-Tau(181); 0.135±0.02 ng/μL) and were further higher compared to elderly controls (Tau; 34.92±6.58 ng/μL, p-Tau(181); 0.122±0.01 ng/ μL). A significant (p<0.0001) downhill correlation was found between Tau as well as p-Tau(181) levels with HMSE and MoCA score. This study for the first time reports the concentration of Tau and p-Tau(181) in serum of AD and MCI patients. The cutoff values of Tau and p-Tau(181) of AD and MCI patients with sensitivity and specificity reveal that serum level of these proteins can be used as a predictive marker for AD and MCI. Public Library of Science 2016-07-26 /pmc/articles/PMC4961391/ /pubmed/27459603 http://dx.doi.org/10.1371/journal.pone.0159099 Text en © 2016 Shekhar et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shekhar, Shashank Kumar, Rahul Rai, Nitish Kumar, Vijay Singh, Kusum Upadhyay, Ashish Datt Tripathi, Manjari Dwivedi, Sadanand Dey, Aparajit B. Dey, Sharmistha Estimation of Tau and Phosphorylated Tau(181) in Serum of Alzheimer’s Disease and Mild Cognitive Impairment Patients |
title | Estimation of Tau and Phosphorylated Tau(181) in Serum of Alzheimer’s Disease and Mild Cognitive Impairment Patients |
title_full | Estimation of Tau and Phosphorylated Tau(181) in Serum of Alzheimer’s Disease and Mild Cognitive Impairment Patients |
title_fullStr | Estimation of Tau and Phosphorylated Tau(181) in Serum of Alzheimer’s Disease and Mild Cognitive Impairment Patients |
title_full_unstemmed | Estimation of Tau and Phosphorylated Tau(181) in Serum of Alzheimer’s Disease and Mild Cognitive Impairment Patients |
title_short | Estimation of Tau and Phosphorylated Tau(181) in Serum of Alzheimer’s Disease and Mild Cognitive Impairment Patients |
title_sort | estimation of tau and phosphorylated tau(181) in serum of alzheimer’s disease and mild cognitive impairment patients |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961391/ https://www.ncbi.nlm.nih.gov/pubmed/27459603 http://dx.doi.org/10.1371/journal.pone.0159099 |
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