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Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells

This research aims to study the production of chitosan from shrimp shell (Litopenaeus vannamei) of waste origin using two chemical methodologies involving demineralization, deproteinization, and the degree of deacetylation. The evaluation of the quality of chitosan from waste shrimp shells includes...

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Autores principales: Vilar Junior, José Carlos, Ribeaux, Daylin Rubio, Alves da Silva, Carlos Alberto, De Campos-Takaki, Galba Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961830/
https://www.ncbi.nlm.nih.gov/pubmed/27478443
http://dx.doi.org/10.1155/2016/5127515
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author Vilar Junior, José Carlos
Ribeaux, Daylin Rubio
Alves da Silva, Carlos Alberto
De Campos-Takaki, Galba Maria
author_facet Vilar Junior, José Carlos
Ribeaux, Daylin Rubio
Alves da Silva, Carlos Alberto
De Campos-Takaki, Galba Maria
author_sort Vilar Junior, José Carlos
collection PubMed
description This research aims to study the production of chitosan from shrimp shell (Litopenaeus vannamei) of waste origin using two chemical methodologies involving demineralization, deproteinization, and the degree of deacetylation. The evaluation of the quality of chitosan from waste shrimp shells includes parameters for the yield, physical chemistry characteristics by infrared spectroscopy (FT-IR), the degree of deacetylation, and antibacterial activity. The results showed (by Method 1) extraction yields for chitin of 33% and for chitosan of 49% and a 76% degree of deacetylation. Chitosan obtained by Method 2 was more efficient: chitin (36%) and chitosan (63%), with a high degree of deacetylation (81.7%). The antibacterial activity was tested against Gram-negative bacteria (Stenotrophomonas maltophilia and Enterobacter cloacae) and Gram-positive Bacillus subtilis and the Minimum Inhibitory Concentrations (MIC) and the Minimum Bactericidal Concentration (MBC) were determined. Method 2 showed that extracted chitosan has good antimicrobial potential against Gram-positive and Gram-negative bacteria and that the process is viable.
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spelling pubmed-49618302016-07-31 Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells Vilar Junior, José Carlos Ribeaux, Daylin Rubio Alves da Silva, Carlos Alberto De Campos-Takaki, Galba Maria Int J Microbiol Research Article This research aims to study the production of chitosan from shrimp shell (Litopenaeus vannamei) of waste origin using two chemical methodologies involving demineralization, deproteinization, and the degree of deacetylation. The evaluation of the quality of chitosan from waste shrimp shells includes parameters for the yield, physical chemistry characteristics by infrared spectroscopy (FT-IR), the degree of deacetylation, and antibacterial activity. The results showed (by Method 1) extraction yields for chitin of 33% and for chitosan of 49% and a 76% degree of deacetylation. Chitosan obtained by Method 2 was more efficient: chitin (36%) and chitosan (63%), with a high degree of deacetylation (81.7%). The antibacterial activity was tested against Gram-negative bacteria (Stenotrophomonas maltophilia and Enterobacter cloacae) and Gram-positive Bacillus subtilis and the Minimum Inhibitory Concentrations (MIC) and the Minimum Bactericidal Concentration (MBC) were determined. Method 2 showed that extracted chitosan has good antimicrobial potential against Gram-positive and Gram-negative bacteria and that the process is viable. Hindawi Publishing Corporation 2016 2016-07-13 /pmc/articles/PMC4961830/ /pubmed/27478443 http://dx.doi.org/10.1155/2016/5127515 Text en Copyright © 2016 José Carlos Vilar Junior et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Vilar Junior, José Carlos
Ribeaux, Daylin Rubio
Alves da Silva, Carlos Alberto
De Campos-Takaki, Galba Maria
Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells
title Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells
title_full Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells
title_fullStr Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells
title_full_unstemmed Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells
title_short Physicochemical and Antibacterial Properties of Chitosan Extracted from Waste Shrimp Shells
title_sort physicochemical and antibacterial properties of chitosan extracted from waste shrimp shells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961830/
https://www.ncbi.nlm.nih.gov/pubmed/27478443
http://dx.doi.org/10.1155/2016/5127515
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