Cargando…
Characterization and Uncertainty Assessment of a Certified Reference Material of Chloramphenicol in Methanol (GBW(E)082557)
Prior to preparation of CRM candidate of chloramphenicol in methanol with a concentration of 100 mg/L, two independent methods including mass balance (MB) and quantitative nuclear magnetic resonance (qNMR) were employed to precisely measure the mass fraction of pure chloramphenicol materials. The ma...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961862/ https://www.ncbi.nlm.nih.gov/pubmed/27493666 http://dx.doi.org/10.1155/2016/2348257 |
_version_ | 1782444722111905792 |
---|---|
author | Yang, Mengrui Wang, Min Zhou, Jian Song, Yinqing Wang, Tongtong |
author_facet | Yang, Mengrui Wang, Min Zhou, Jian Song, Yinqing Wang, Tongtong |
author_sort | Yang, Mengrui |
collection | PubMed |
description | Prior to preparation of CRM candidate of chloramphenicol in methanol with a concentration of 100 mg/L, two independent methods including mass balance (MB) and quantitative nuclear magnetic resonance (qNMR) were employed to precisely measure the mass fraction of pure chloramphenicol materials. The mass fraction was assigned to be 99.8% with uncertainty of 0.3%. Homogeneity testing and stability study of chloramphenicol in methanol were examined by using high performance liquid chromatography. Additionally, the uncertainties originating from the process of CRM development were comprehensively evaluated. The experimental results indicate that the property value of this CRM is homogeneous and stable at 4°C for at least six months. The new CRM (GBW(E)082557) can be applicable to calibration of instrument and assurance of accuracy and comparability of results in routine measurement. |
format | Online Article Text |
id | pubmed-4961862 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-49618622016-08-04 Characterization and Uncertainty Assessment of a Certified Reference Material of Chloramphenicol in Methanol (GBW(E)082557) Yang, Mengrui Wang, Min Zhou, Jian Song, Yinqing Wang, Tongtong Int J Anal Chem Research Article Prior to preparation of CRM candidate of chloramphenicol in methanol with a concentration of 100 mg/L, two independent methods including mass balance (MB) and quantitative nuclear magnetic resonance (qNMR) were employed to precisely measure the mass fraction of pure chloramphenicol materials. The mass fraction was assigned to be 99.8% with uncertainty of 0.3%. Homogeneity testing and stability study of chloramphenicol in methanol were examined by using high performance liquid chromatography. Additionally, the uncertainties originating from the process of CRM development were comprehensively evaluated. The experimental results indicate that the property value of this CRM is homogeneous and stable at 4°C for at least six months. The new CRM (GBW(E)082557) can be applicable to calibration of instrument and assurance of accuracy and comparability of results in routine measurement. Hindawi Publishing Corporation 2016 2016-07-13 /pmc/articles/PMC4961862/ /pubmed/27493666 http://dx.doi.org/10.1155/2016/2348257 Text en Copyright © 2016 Mengrui Yang et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Yang, Mengrui Wang, Min Zhou, Jian Song, Yinqing Wang, Tongtong Characterization and Uncertainty Assessment of a Certified Reference Material of Chloramphenicol in Methanol (GBW(E)082557) |
title | Characterization and Uncertainty Assessment of a Certified Reference Material of Chloramphenicol in Methanol (GBW(E)082557) |
title_full | Characterization and Uncertainty Assessment of a Certified Reference Material of Chloramphenicol in Methanol (GBW(E)082557) |
title_fullStr | Characterization and Uncertainty Assessment of a Certified Reference Material of Chloramphenicol in Methanol (GBW(E)082557) |
title_full_unstemmed | Characterization and Uncertainty Assessment of a Certified Reference Material of Chloramphenicol in Methanol (GBW(E)082557) |
title_short | Characterization and Uncertainty Assessment of a Certified Reference Material of Chloramphenicol in Methanol (GBW(E)082557) |
title_sort | characterization and uncertainty assessment of a certified reference material of chloramphenicol in methanol (gbw(e)082557) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961862/ https://www.ncbi.nlm.nih.gov/pubmed/27493666 http://dx.doi.org/10.1155/2016/2348257 |
work_keys_str_mv | AT yangmengrui characterizationanduncertaintyassessmentofacertifiedreferencematerialofchloramphenicolinmethanolgbwe082557 AT wangmin characterizationanduncertaintyassessmentofacertifiedreferencematerialofchloramphenicolinmethanolgbwe082557 AT zhoujian characterizationanduncertaintyassessmentofacertifiedreferencematerialofchloramphenicolinmethanolgbwe082557 AT songyinqing characterizationanduncertaintyassessmentofacertifiedreferencematerialofchloramphenicolinmethanolgbwe082557 AT wangtongtong characterizationanduncertaintyassessmentofacertifiedreferencematerialofchloramphenicolinmethanolgbwe082557 |