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Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes

This work describes that cytotoxicity of lead chloride and lead acetate to in vitro cultured lymphocytes from human umbilical cord blood, using four monitoring methods namely, trypan blue staining, acridine orange/ethidium bromide staining, 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromi...

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Autores principales: Patnaik, Rajashree, Padhy, Rabindra N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Slovak Toxicology Society SETOX 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961924/
https://www.ncbi.nlm.nih.gov/pubmed/27486358
http://dx.doi.org/10.1515/intox-2015-0007
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author Patnaik, Rajashree
Padhy, Rabindra N.
author_facet Patnaik, Rajashree
Padhy, Rabindra N.
author_sort Patnaik, Rajashree
collection PubMed
description This work describes that cytotoxicity of lead chloride and lead acetate to in vitro cultured lymphocytes from human umbilical cord blood, using four monitoring methods namely, trypan blue staining, acridine orange/ethidium bromide staining, 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromide (MTT) and neutral red uptake assays; lead genotoxicity to lymphocytes was monitored by comet assay. The MIC value in each method was invariably 300 mg/L for PbCl(2). Lethal concentration(25) (LC(25)) values were almost in an agreeable range: 691.83 to 831.76 mg/L; LC(50) values in each method were almost in the range: 1174.9 to 1348.9 mg/L; LC(100) values were in the range: 3000 to 3300 mg/L, for lead chloride. Similarly, The MIC value in each method were invariably 150 mg/L; LC(25) values were almost in the range: 295.12 to 371.53 mg/L; LC(50) values were in the range: 501.18 to 588.84 mg/L; LC(100) value was 1500 mg/L in all assays, for lead acetate. The comet assay also indicated that the LC(100) values were 3300 mg/L lead chloride and 1500 mg/L lead acetate. Thus, both cytotoxicity and genotoxicity were recorded at 3300 mg/L lead chloride and 1500 mg/L lead acetate with lymphocytes.
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spelling pubmed-49619242016-08-02 Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes Patnaik, Rajashree Padhy, Rabindra N. Interdiscip Toxicol Original Article This work describes that cytotoxicity of lead chloride and lead acetate to in vitro cultured lymphocytes from human umbilical cord blood, using four monitoring methods namely, trypan blue staining, acridine orange/ethidium bromide staining, 3-[4,5-dimethylthiazol-2-yl] 2,5-diphenyl tetrazolium bromide (MTT) and neutral red uptake assays; lead genotoxicity to lymphocytes was monitored by comet assay. The MIC value in each method was invariably 300 mg/L for PbCl(2). Lethal concentration(25) (LC(25)) values were almost in an agreeable range: 691.83 to 831.76 mg/L; LC(50) values in each method were almost in the range: 1174.9 to 1348.9 mg/L; LC(100) values were in the range: 3000 to 3300 mg/L, for lead chloride. Similarly, The MIC value in each method were invariably 150 mg/L; LC(25) values were almost in the range: 295.12 to 371.53 mg/L; LC(50) values were in the range: 501.18 to 588.84 mg/L; LC(100) value was 1500 mg/L in all assays, for lead acetate. The comet assay also indicated that the LC(100) values were 3300 mg/L lead chloride and 1500 mg/L lead acetate. Thus, both cytotoxicity and genotoxicity were recorded at 3300 mg/L lead chloride and 1500 mg/L lead acetate with lymphocytes. Slovak Toxicology Society SETOX 2015-03 2015-03 /pmc/articles/PMC4961924/ /pubmed/27486358 http://dx.doi.org/10.1515/intox-2015-0007 Text en Copyright © 2015 SETOX & Institute of Experimental Pharmacology and Toxicology, SASc. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Patnaik, Rajashree
Padhy, Rabindra N.
Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes
title Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes
title_full Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes
title_fullStr Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes
title_full_unstemmed Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes
title_short Probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes
title_sort probit analysis of comparative assays on toxicities of lead chloride and lead acetate to in vitro cultured human umbilical cord blood lymphocytes
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4961924/
https://www.ncbi.nlm.nih.gov/pubmed/27486358
http://dx.doi.org/10.1515/intox-2015-0007
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