Cargando…

Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves

BACKGROUND: Callistemon citrinus (Curtis.) (Family- Myrtaceae) is a popular evergreen shrub in Bangladesh. In the present study, the leaves of this plant have been assessed comprehensively for free radical scavenging, thrombolytic and membrane stabilizing activities. METHODS: The leaves were collect...

Descripción completa

Detalles Bibliográficos
Autores principales: Ahmed, Farhana, Rahman, Mohammad Sharifur
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962397/
https://www.ncbi.nlm.nih.gov/pubmed/27460997
http://dx.doi.org/10.1186/s12906-016-1239-1
_version_ 1782444826730430464
author Ahmed, Farhana
Rahman, Mohammad Sharifur
author_facet Ahmed, Farhana
Rahman, Mohammad Sharifur
author_sort Ahmed, Farhana
collection PubMed
description BACKGROUND: Callistemon citrinus (Curtis.) (Family- Myrtaceae) is a popular evergreen shrub in Bangladesh. In the present study, the leaves of this plant have been assessed comprehensively for free radical scavenging, thrombolytic and membrane stabilizing activities. METHODS: The leaves were collected, powdered and extracted with methanol. The extract was then concentrated and successively fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions. The extractives were investigated for free radical scavenging, thrombolytic and membrane stabilizing activities. RESULTS: In case of 1,1 diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide radical scavenging assays, the crude methanol extract of the leaves showed the highest free radical scavenging activity among the tested materials including standard ascorbic acid (p = 0.0000). Besides, this extract was also found significantly rich (p = 0.0000) in phenolics and flavonoids compared to other organic fractions. In thrombolytic study, the petroleum ether fraction exhibited significantly stronger thrombolysis (p = 0.024) than other leaf extractives but was weaker than the standard streptokinase. In membrane stabilizing assay, the activity of chloroform fraction was similar to that of standard acetylsalicylic acid (p = 1.000) in hypotonic solution induced hemolysis. However, membrane stabilization activity of this chloroform fraction was found significantly stronger than that of the standard (p = 0.0000) in heat induced hemolysis. CONCLUSION: This study has revealed the medicinal capabilities of different organic fractions of C. citrinus displaying free radical scavenging, thrombolysis and membrane stabilizing antiinflammatory potentials. Further bioactivity guided isolation is required to obtain pharmacologically secondary metabolites.
format Online
Article
Text
id pubmed-4962397
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-49623972016-07-28 Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves Ahmed, Farhana Rahman, Mohammad Sharifur BMC Complement Altern Med Research Article BACKGROUND: Callistemon citrinus (Curtis.) (Family- Myrtaceae) is a popular evergreen shrub in Bangladesh. In the present study, the leaves of this plant have been assessed comprehensively for free radical scavenging, thrombolytic and membrane stabilizing activities. METHODS: The leaves were collected, powdered and extracted with methanol. The extract was then concentrated and successively fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions. The extractives were investigated for free radical scavenging, thrombolytic and membrane stabilizing activities. RESULTS: In case of 1,1 diphenyl-2-picrylhydrazyl (DPPH) and hydrogen peroxide radical scavenging assays, the crude methanol extract of the leaves showed the highest free radical scavenging activity among the tested materials including standard ascorbic acid (p = 0.0000). Besides, this extract was also found significantly rich (p = 0.0000) in phenolics and flavonoids compared to other organic fractions. In thrombolytic study, the petroleum ether fraction exhibited significantly stronger thrombolysis (p = 0.024) than other leaf extractives but was weaker than the standard streptokinase. In membrane stabilizing assay, the activity of chloroform fraction was similar to that of standard acetylsalicylic acid (p = 1.000) in hypotonic solution induced hemolysis. However, membrane stabilization activity of this chloroform fraction was found significantly stronger than that of the standard (p = 0.0000) in heat induced hemolysis. CONCLUSION: This study has revealed the medicinal capabilities of different organic fractions of C. citrinus displaying free radical scavenging, thrombolysis and membrane stabilizing antiinflammatory potentials. Further bioactivity guided isolation is required to obtain pharmacologically secondary metabolites. BioMed Central 2016-07-26 /pmc/articles/PMC4962397/ /pubmed/27460997 http://dx.doi.org/10.1186/s12906-016-1239-1 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Ahmed, Farhana
Rahman, Mohammad Sharifur
Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves
title Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves
title_full Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves
title_fullStr Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves
title_full_unstemmed Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves
title_short Preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of Callistemon citrinus (Curtis.) skeels leaves
title_sort preliminary assessment of free radical scavenging, thrombolytic and membrane stabilizing capabilities of organic fractions of callistemon citrinus (curtis.) skeels leaves
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962397/
https://www.ncbi.nlm.nih.gov/pubmed/27460997
http://dx.doi.org/10.1186/s12906-016-1239-1
work_keys_str_mv AT ahmedfarhana preliminaryassessmentoffreeradicalscavengingthrombolyticandmembranestabilizingcapabilitiesoforganicfractionsofcallistemoncitrinuscurtisskeelsleaves
AT rahmanmohammadsharifur preliminaryassessmentoffreeradicalscavengingthrombolyticandmembranestabilizingcapabilitiesoforganicfractionsofcallistemoncitrinuscurtisskeelsleaves