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The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction

BACKGROUND: Synaptic transmission requires both pre- and post-synaptic elements for neural communication. The postsynaptic structure contributes to the ability of synaptic currents to induce voltage changes in postsynaptic cells. At the Drosophila neuromuscular junction (NMJ), the postsynaptic struc...

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Autores principales: Nguyen, Christine T., Stewart, Bryan A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962461/
https://www.ncbi.nlm.nih.gov/pubmed/27459966
http://dx.doi.org/10.1186/s12868-016-0290-7
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author Nguyen, Christine T.
Stewart, Bryan A.
author_facet Nguyen, Christine T.
Stewart, Bryan A.
author_sort Nguyen, Christine T.
collection PubMed
description BACKGROUND: Synaptic transmission requires both pre- and post-synaptic elements for neural communication. The postsynaptic structure contributes to the ability of synaptic currents to induce voltage changes in postsynaptic cells. At the Drosophila neuromuscular junction (NMJ), the postsynaptic structure, known as the subsynaptic reticulum (SSR), consists of elaborate membrane folds that link the synaptic contacts to the muscle, but its role in synaptic physiology is poorly understood. RESULTS: In this study, we investigate the role of the SSR with simultaneous intra- and extra-cellular recordings that allow us to identify the origin of spontaneously occurring synaptic events. We compare data from Type 1b and 1s synaptic boutons, which have naturally occurring variations of the SSR, as well as from genetic mutants that up or down-regulate SSR complexity. We observed that some synaptic currents do not result in postsynaptic voltage changes, events we called ‘missing quanta’. The frequency of missing quanta is positively correlated with SSR complexity in both natural and genetically-induced variants. Rise-time and amplitude data suggest that passive membrane properties contribute to the observed differences in synaptic effectiveness. CONCLUSION: We conclude that electrotonic decay within the postsynaptic structure contributes to the phenomenon of missing quanta. Further studies directed at understanding the role of the SSR in synaptic transmission and the potential for regulating ‘missing quanta’ will yield important information about synaptic transmission at the Drosophila NMJ.
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spelling pubmed-49624612016-07-28 The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction Nguyen, Christine T. Stewart, Bryan A. BMC Neurosci Research Article BACKGROUND: Synaptic transmission requires both pre- and post-synaptic elements for neural communication. The postsynaptic structure contributes to the ability of synaptic currents to induce voltage changes in postsynaptic cells. At the Drosophila neuromuscular junction (NMJ), the postsynaptic structure, known as the subsynaptic reticulum (SSR), consists of elaborate membrane folds that link the synaptic contacts to the muscle, but its role in synaptic physiology is poorly understood. RESULTS: In this study, we investigate the role of the SSR with simultaneous intra- and extra-cellular recordings that allow us to identify the origin of spontaneously occurring synaptic events. We compare data from Type 1b and 1s synaptic boutons, which have naturally occurring variations of the SSR, as well as from genetic mutants that up or down-regulate SSR complexity. We observed that some synaptic currents do not result in postsynaptic voltage changes, events we called ‘missing quanta’. The frequency of missing quanta is positively correlated with SSR complexity in both natural and genetically-induced variants. Rise-time and amplitude data suggest that passive membrane properties contribute to the observed differences in synaptic effectiveness. CONCLUSION: We conclude that electrotonic decay within the postsynaptic structure contributes to the phenomenon of missing quanta. Further studies directed at understanding the role of the SSR in synaptic transmission and the potential for regulating ‘missing quanta’ will yield important information about synaptic transmission at the Drosophila NMJ. BioMed Central 2016-07-26 /pmc/articles/PMC4962461/ /pubmed/27459966 http://dx.doi.org/10.1186/s12868-016-0290-7 Text en © The Author(s) 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Nguyen, Christine T.
Stewart, Bryan A.
The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction
title The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction
title_full The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction
title_fullStr The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction
title_full_unstemmed The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction
title_short The influence of postsynaptic structure on missing quanta at the Drosophila neuromuscular junction
title_sort influence of postsynaptic structure on missing quanta at the drosophila neuromuscular junction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962461/
https://www.ncbi.nlm.nih.gov/pubmed/27459966
http://dx.doi.org/10.1186/s12868-016-0290-7
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