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miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1
MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following s...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962562/ https://www.ncbi.nlm.nih.gov/pubmed/27482232 http://dx.doi.org/10.4103/1673-5374.184504 |
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author | Qian, Tian-mei Zhao, Li-li Wang, Jing Li, Ping Qin, Jing Liu, Yi-sheng Yu, Bin Ding, Fei Gu, Xiao-song Zhou, Song-lin |
author_facet | Qian, Tian-mei Zhao, Li-li Wang, Jing Li, Ping Qin, Jing Liu, Yi-sheng Yu, Bin Ding, Fei Gu, Xiao-song Zhou, Song-lin |
author_sort | Qian, Tian-mei |
collection | PubMed |
description | MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction, thereby affecting proliferation and migration of Schwann cells. This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1 (Cand1). Up-regulated expression of miR-148b-3p promoted Schwann cell migration, whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro. Further experiments confirmed that Cand1 was a direct target of miR-148b-3p, and Cand1 knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration. These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Cand1 in vitro. |
format | Online Article Text |
id | pubmed-4962562 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-49625622016-08-01 miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1 Qian, Tian-mei Zhao, Li-li Wang, Jing Li, Ping Qin, Jing Liu, Yi-sheng Yu, Bin Ding, Fei Gu, Xiao-song Zhou, Song-lin Neural Regen Res Research Article MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction, thereby affecting proliferation and migration of Schwann cells. This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1 (Cand1). Up-regulated expression of miR-148b-3p promoted Schwann cell migration, whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro. Further experiments confirmed that Cand1 was a direct target of miR-148b-3p, and Cand1 knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration. These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Cand1 in vitro. Medknow Publications & Media Pvt Ltd 2016-06 /pmc/articles/PMC4962562/ /pubmed/27482232 http://dx.doi.org/10.4103/1673-5374.184504 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms. |
spellingShingle | Research Article Qian, Tian-mei Zhao, Li-li Wang, Jing Li, Ping Qin, Jing Liu, Yi-sheng Yu, Bin Ding, Fei Gu, Xiao-song Zhou, Song-lin miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1 |
title | miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1 |
title_full | miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1 |
title_fullStr | miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1 |
title_full_unstemmed | miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1 |
title_short | miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1 |
title_sort | mir-148b-3p promotes migration of schwann cells by targeting cullin-associated and neddylation-dissociated 1 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962562/ https://www.ncbi.nlm.nih.gov/pubmed/27482232 http://dx.doi.org/10.4103/1673-5374.184504 |
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