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miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1

MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following s...

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Autores principales: Qian, Tian-mei, Zhao, Li-li, Wang, Jing, Li, Ping, Qin, Jing, Liu, Yi-sheng, Yu, Bin, Ding, Fei, Gu, Xiao-song, Zhou, Song-lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962562/
https://www.ncbi.nlm.nih.gov/pubmed/27482232
http://dx.doi.org/10.4103/1673-5374.184504
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author Qian, Tian-mei
Zhao, Li-li
Wang, Jing
Li, Ping
Qin, Jing
Liu, Yi-sheng
Yu, Bin
Ding, Fei
Gu, Xiao-song
Zhou, Song-lin
author_facet Qian, Tian-mei
Zhao, Li-li
Wang, Jing
Li, Ping
Qin, Jing
Liu, Yi-sheng
Yu, Bin
Ding, Fei
Gu, Xiao-song
Zhou, Song-lin
author_sort Qian, Tian-mei
collection PubMed
description MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction, thereby affecting proliferation and migration of Schwann cells. This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1 (Cand1). Up-regulated expression of miR-148b-3p promoted Schwann cell migration, whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro. Further experiments confirmed that Cand1 was a direct target of miR-148b-3p, and Cand1 knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration. These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Cand1 in vitro.
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spelling pubmed-49625622016-08-01 miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1 Qian, Tian-mei Zhao, Li-li Wang, Jing Li, Ping Qin, Jing Liu, Yi-sheng Yu, Bin Ding, Fei Gu, Xiao-song Zhou, Song-lin Neural Regen Res Research Article MicroRNAs (miRNAs) are small, non-coding RNAs that negatively adjust gene expression in multifarious biological processes. However, the regulatory effects of miRNAs on Schwann cells remain poorly understood. Previous microarray analysis results have shown that miRNA expression is altered following sciatic nerve transaction, thereby affecting proliferation and migration of Schwann cells. This study investigated whether miR-148b-3p could regulate migration of Schwann cells by directly targeting cullin-associated and neddylation-dissociated 1 (Cand1). Up-regulated expression of miR-148b-3p promoted Schwann cell migration, whereas silencing of miR-148b-3p inhibited Schwann cell migration in vitro. Further experiments confirmed that Cand1 was a direct target of miR-148b-3p, and Cand1 knockdown reversed suppression of the miR-148b-3p inhibitor on Schwann cell migration. These results suggested that miR-148b-3p promoted migration of Schwann cells by directly targeting Cand1 in vitro. Medknow Publications & Media Pvt Ltd 2016-06 /pmc/articles/PMC4962562/ /pubmed/27482232 http://dx.doi.org/10.4103/1673-5374.184504 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.
spellingShingle Research Article
Qian, Tian-mei
Zhao, Li-li
Wang, Jing
Li, Ping
Qin, Jing
Liu, Yi-sheng
Yu, Bin
Ding, Fei
Gu, Xiao-song
Zhou, Song-lin
miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1
title miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1
title_full miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1
title_fullStr miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1
title_full_unstemmed miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1
title_short miR-148b-3p promotes migration of Schwann cells by targeting cullin-associated and neddylation-dissociated 1
title_sort mir-148b-3p promotes migration of schwann cells by targeting cullin-associated and neddylation-dissociated 1
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962562/
https://www.ncbi.nlm.nih.gov/pubmed/27482232
http://dx.doi.org/10.4103/1673-5374.184504
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