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Immunomodulation of T(H)2 biased immunity with mucosal administration of nanoemulsion adjuvant
T(H)2-biased immune responses are associated with inadequate protection against some pathogens and with cancer, colitis, asthma and allergy. Since most currently used vaccine adjuvants induce a T(H)2-biased response, this has led to interest in developing adjuvants capable of activating T(H)1 immuni...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962973/ https://www.ncbi.nlm.nih.gov/pubmed/27317451 http://dx.doi.org/10.1016/j.vaccine.2016.06.043 |
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author | Bielinska, Anna U. O’Konek, Jessica J. Janczak, Katarzyna W. Baker, James R. |
author_facet | Bielinska, Anna U. O’Konek, Jessica J. Janczak, Katarzyna W. Baker, James R. |
author_sort | Bielinska, Anna U. |
collection | PubMed |
description | T(H)2-biased immune responses are associated with inadequate protection against some pathogens and with cancer, colitis, asthma and allergy. Since most currently used vaccine adjuvants induce a T(H)2-biased response, this has led to interest in developing adjuvants capable of activating T(H)1 immunity and modulating existing T(H)2 responses. Immunotherapies to shift immune responses from T(H)2 to T(H)1 have generally required prolonged immunization protocols and have not induced effective T(H)1 responses. We have demonstrated that nanoscale emulsions (NE), a novel mucosal adjuvant, induce robust IgA and IgG antibody responses and T(H)1/T(H)17 cellular immunity resulting in protection against a variety of respiratory and mucosal infections. Because intranasal (i.n.) delivery of NE adjuvant consistently induces T(H)1/T(H)17 biased responses, we hypothesized that NE could be used as a therapeutic vaccine to redirect existing T(H)2 polarized immunity towards a more balanced T(H)1/T(H)2 profile. To test this, a T(H)2 immune response was established by intramuscular immunization of mice with alum-adjuvanted hepatitis B surface antigen (HBs), followed by a single subsequent i.n. immunization with NE-HBs. These animals exhibited increased T(H)1 associated immune responses and IL-17, and decreased T(H)2 cytokines (IL-4 and IL-5) and IgG1. NE immunization induced regulatory T cells and IL-10, and IL-10 was required for the suppression of T(H)2 immunity. These data demonstrate that NE-based vaccines can modulate existing T(H)2 immune responses to promote T(H)1/T(H)17 immunity and suggest the potential therapeutic use of NE vaccines for diseases associated with T(H)2 immunity. |
format | Online Article Text |
id | pubmed-4962973 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Elsevier Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49629732016-08-03 Immunomodulation of T(H)2 biased immunity with mucosal administration of nanoemulsion adjuvant Bielinska, Anna U. O’Konek, Jessica J. Janczak, Katarzyna W. Baker, James R. Vaccine Article T(H)2-biased immune responses are associated with inadequate protection against some pathogens and with cancer, colitis, asthma and allergy. Since most currently used vaccine adjuvants induce a T(H)2-biased response, this has led to interest in developing adjuvants capable of activating T(H)1 immunity and modulating existing T(H)2 responses. Immunotherapies to shift immune responses from T(H)2 to T(H)1 have generally required prolonged immunization protocols and have not induced effective T(H)1 responses. We have demonstrated that nanoscale emulsions (NE), a novel mucosal adjuvant, induce robust IgA and IgG antibody responses and T(H)1/T(H)17 cellular immunity resulting in protection against a variety of respiratory and mucosal infections. Because intranasal (i.n.) delivery of NE adjuvant consistently induces T(H)1/T(H)17 biased responses, we hypothesized that NE could be used as a therapeutic vaccine to redirect existing T(H)2 polarized immunity towards a more balanced T(H)1/T(H)2 profile. To test this, a T(H)2 immune response was established by intramuscular immunization of mice with alum-adjuvanted hepatitis B surface antigen (HBs), followed by a single subsequent i.n. immunization with NE-HBs. These animals exhibited increased T(H)1 associated immune responses and IL-17, and decreased T(H)2 cytokines (IL-4 and IL-5) and IgG1. NE immunization induced regulatory T cells and IL-10, and IL-10 was required for the suppression of T(H)2 immunity. These data demonstrate that NE-based vaccines can modulate existing T(H)2 immune responses to promote T(H)1/T(H)17 immunity and suggest the potential therapeutic use of NE vaccines for diseases associated with T(H)2 immunity. Elsevier Science 2016-07-25 /pmc/articles/PMC4962973/ /pubmed/27317451 http://dx.doi.org/10.1016/j.vaccine.2016.06.043 Text en © 2016 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bielinska, Anna U. O’Konek, Jessica J. Janczak, Katarzyna W. Baker, James R. Immunomodulation of T(H)2 biased immunity with mucosal administration of nanoemulsion adjuvant |
title | Immunomodulation of T(H)2 biased immunity with mucosal administration of nanoemulsion adjuvant |
title_full | Immunomodulation of T(H)2 biased immunity with mucosal administration of nanoemulsion adjuvant |
title_fullStr | Immunomodulation of T(H)2 biased immunity with mucosal administration of nanoemulsion adjuvant |
title_full_unstemmed | Immunomodulation of T(H)2 biased immunity with mucosal administration of nanoemulsion adjuvant |
title_short | Immunomodulation of T(H)2 biased immunity with mucosal administration of nanoemulsion adjuvant |
title_sort | immunomodulation of t(h)2 biased immunity with mucosal administration of nanoemulsion adjuvant |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4962973/ https://www.ncbi.nlm.nih.gov/pubmed/27317451 http://dx.doi.org/10.1016/j.vaccine.2016.06.043 |
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