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KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation
Studies have suggested that Epithelial–Mesenchymal Transition (EMT) and transformation is an important step in progression to cancer. Par3 (partitioning-defective protein) is a crucial factor in regulating epithelial cell polarity. However, the mechanism by which the latency associated nuclear antig...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963126/ https://www.ncbi.nlm.nih.gov/pubmed/27463802 http://dx.doi.org/10.1371/journal.ppat.1005801 |
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author | Jha, Hem C. Sun, Zhiguo Upadhyay, Santosh K. El-Naccache, Darine W. Singh, Rajnish K. Sahu, Sushil K. Robertson, Erle S. |
author_facet | Jha, Hem C. Sun, Zhiguo Upadhyay, Santosh K. El-Naccache, Darine W. Singh, Rajnish K. Sahu, Sushil K. Robertson, Erle S. |
author_sort | Jha, Hem C. |
collection | PubMed |
description | Studies have suggested that Epithelial–Mesenchymal Transition (EMT) and transformation is an important step in progression to cancer. Par3 (partitioning-defective protein) is a crucial factor in regulating epithelial cell polarity. However, the mechanism by which the latency associated nuclear antigen (LANA) encoded by Kaposi's Sarcoma associated herpesvirus (KSHV) regulates Par3 and EMTs markers (Epithelial-Mesenchymal Transition) during viral-mediated B-cell oncogenesis has not been fully explored. Moreover, several studies have demonstrated a crucial role for EMT markers during B-cell malignancies. In this study, we demonstrate that Par3 is significantly up-regulated in KSHV-infected primary B-cells. Further, Par3 interacted with LANA in KSHV positive and LANA expressing cells which led to translocation of Par3 from the cell periphery to a predominantly nuclear signal. Par3 knockdown led to reduced cell proliferation and increased apoptotic induction. Levels of SNAIL was elevated, and E-cadherin was reduced in the presence of LANA or Par3. Interestingly, KSHV infection in primary B-cells led to enhancement of SNAIL and down-regulation of E-cadherin in a temporal manner. Importantly, knockdown of SNAIL, a major EMT regulator, in KSHV cells resulted in reduced expression of LANA, Par3, and enhanced E-cadherin. Also, SNAIL bound to the promoter region of p21 and can regulate its activity. Further a SNAIL inhibitor diminished NF-kB signaling through upregulation of Caspase3 in KSHV positive cells in vitro. This was also supported by upregulation of SNAIL and Par3 in BC-3 transplanted NOD-SCID mice which has potential as a therapeutic target for KSHV-associated B-cell lymphomas. |
format | Online Article Text |
id | pubmed-4963126 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49631262016-08-08 KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation Jha, Hem C. Sun, Zhiguo Upadhyay, Santosh K. El-Naccache, Darine W. Singh, Rajnish K. Sahu, Sushil K. Robertson, Erle S. PLoS Pathog Research Article Studies have suggested that Epithelial–Mesenchymal Transition (EMT) and transformation is an important step in progression to cancer. Par3 (partitioning-defective protein) is a crucial factor in regulating epithelial cell polarity. However, the mechanism by which the latency associated nuclear antigen (LANA) encoded by Kaposi's Sarcoma associated herpesvirus (KSHV) regulates Par3 and EMTs markers (Epithelial-Mesenchymal Transition) during viral-mediated B-cell oncogenesis has not been fully explored. Moreover, several studies have demonstrated a crucial role for EMT markers during B-cell malignancies. In this study, we demonstrate that Par3 is significantly up-regulated in KSHV-infected primary B-cells. Further, Par3 interacted with LANA in KSHV positive and LANA expressing cells which led to translocation of Par3 from the cell periphery to a predominantly nuclear signal. Par3 knockdown led to reduced cell proliferation and increased apoptotic induction. Levels of SNAIL was elevated, and E-cadherin was reduced in the presence of LANA or Par3. Interestingly, KSHV infection in primary B-cells led to enhancement of SNAIL and down-regulation of E-cadherin in a temporal manner. Importantly, knockdown of SNAIL, a major EMT regulator, in KSHV cells resulted in reduced expression of LANA, Par3, and enhanced E-cadherin. Also, SNAIL bound to the promoter region of p21 and can regulate its activity. Further a SNAIL inhibitor diminished NF-kB signaling through upregulation of Caspase3 in KSHV positive cells in vitro. This was also supported by upregulation of SNAIL and Par3 in BC-3 transplanted NOD-SCID mice which has potential as a therapeutic target for KSHV-associated B-cell lymphomas. Public Library of Science 2016-07-27 /pmc/articles/PMC4963126/ /pubmed/27463802 http://dx.doi.org/10.1371/journal.ppat.1005801 Text en © 2016 Jha et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Jha, Hem C. Sun, Zhiguo Upadhyay, Santosh K. El-Naccache, Darine W. Singh, Rajnish K. Sahu, Sushil K. Robertson, Erle S. KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation |
title | KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation |
title_full | KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation |
title_fullStr | KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation |
title_full_unstemmed | KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation |
title_short | KSHV-Mediated Regulation of Par3 and SNAIL Contributes to B-Cell Proliferation |
title_sort | kshv-mediated regulation of par3 and snail contributes to b-cell proliferation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963126/ https://www.ncbi.nlm.nih.gov/pubmed/27463802 http://dx.doi.org/10.1371/journal.ppat.1005801 |
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