PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18

Six multiplex-compatible PCR primers were designed to distinguish Streptococcus pneumoniae serotypes within serogroup 18 from culturable/nonculturable pneumococcal specimens, with no cross-reactivity with other serotypes and respiratory organisms. These primers will aid in the generation of better d...

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Autores principales: Tanmoy, Arif M., Saha, Senjuti, Darmstadt, Gary L., Whitney, Cynthia G., Saha, Samir K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2016
Materias:
Epidemiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963509/
https://www.ncbi.nlm.nih.gov/pubmed/27252464
http://dx.doi.org/10.1128/JCM.00419-16
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author Tanmoy, Arif M.
Saha, Senjuti
Darmstadt, Gary L.
Whitney, Cynthia G.
Saha, Samir K.
author_facet Tanmoy, Arif M.
Saha, Senjuti
Darmstadt, Gary L.
Whitney, Cynthia G.
Saha, Samir K.
author_sort Tanmoy, Arif M.
collection PubMed
description Six multiplex-compatible PCR primers were designed to distinguish Streptococcus pneumoniae serotypes within serogroup 18 from culturable/nonculturable pneumococcal specimens, with no cross-reactivity with other serotypes and respiratory organisms. These primers will aid in the generation of better data on vaccine/nonvaccine serotypes in invasive and carriage pneumococcal surveillance and contribute to future vaccine formulation and impact studies.
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spelling pubmed-49635092016-08-08 PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18 Tanmoy, Arif M. Saha, Senjuti Darmstadt, Gary L. Whitney, Cynthia G. Saha, Samir K. J Clin Microbiol Epidemiology Six multiplex-compatible PCR primers were designed to distinguish Streptococcus pneumoniae serotypes within serogroup 18 from culturable/nonculturable pneumococcal specimens, with no cross-reactivity with other serotypes and respiratory organisms. These primers will aid in the generation of better data on vaccine/nonvaccine serotypes in invasive and carriage pneumococcal surveillance and contribute to future vaccine formulation and impact studies. American Society for Microbiology 2016-07-25 2016-08 /pmc/articles/PMC4963509/ /pubmed/27252464 http://dx.doi.org/10.1128/JCM.00419-16 Text en Copyright © 2016 Tanmoy et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (http://creativecommons.org/licenses/by/4.0/) .
spellingShingle Epidemiology
Tanmoy, Arif M.
Saha, Senjuti
Darmstadt, Gary L.
Whitney, Cynthia G.
Saha, Samir K.
PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18
title PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18
title_full PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18
title_fullStr PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18
title_full_unstemmed PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18
title_short PCR-Based Serotyping of Streptococcus pneumoniae from Culture-Negative Specimens: Novel Primers for Detection of Serotypes within Serogroup 18
title_sort pcr-based serotyping of streptococcus pneumoniae from culture-negative specimens: novel primers for detection of serotypes within serogroup 18
topic Epidemiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963509/
https://www.ncbi.nlm.nih.gov/pubmed/27252464
http://dx.doi.org/10.1128/JCM.00419-16
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