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Distribution of Triamcinolone Acetonide after Intravitreal Injection into Silicone Oil-Filled Eye

There is increasing use of the vitreous cavity as a reservoir for drug delivery. We study the intraocular migration and distribution of triamcinolone acetonide (TA) after injection into silicone oil tamponade agent during and after vitrectomy surgery ex vivo (pig eye) and in vitro (glass bottle). Fo...

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Detalles Bibliográficos
Autores principales: Da, Ma, Li, Kenneth K. W., Chan, Kevin C., Wu, Ed X., Wong, David S. H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963566/
https://www.ncbi.nlm.nih.gov/pubmed/27493959
http://dx.doi.org/10.1155/2016/5485467
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author Da, Ma
Li, Kenneth K. W.
Chan, Kevin C.
Wu, Ed X.
Wong, David S. H.
author_facet Da, Ma
Li, Kenneth K. W.
Chan, Kevin C.
Wu, Ed X.
Wong, David S. H.
author_sort Da, Ma
collection PubMed
description There is increasing use of the vitreous cavity as a reservoir for drug delivery. We study the intraocular migration and distribution of triamcinolone acetonide (TA) after injection into silicone oil tamponade agent during and after vitrectomy surgery ex vivo (pig eye) and in vitro (glass bottle). For ex vivo assessment, intraocular migration of TA was imaged using real-time FLASH MRI scans and high-resolution T2W imaging and the in vitro model was monitored continuously with a video camera. Results of the ex vivo experiment showed that the TA droplet sank to the interface of silicone oil and aqueous almost immediately after injection and remained inside the silicone oil bubble for as long as 16 minutes. The in vitro results showed that, after the shrinkage of the droplet, TA gradually precipitated leaving only a lump of whitish crystalline residue inside the droplet for about 100 minutes. TA then quickly broke the interface and dispersed into the underlying aqueous within 15 seconds, which may result in a momentary increase of local TA concentration in the aqueous portion and potentially toxic to the retina. Our study suggests that silicone oil may not be a good candidate as a drug reservoir for drugs like TA.
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spelling pubmed-49635662016-08-04 Distribution of Triamcinolone Acetonide after Intravitreal Injection into Silicone Oil-Filled Eye Da, Ma Li, Kenneth K. W. Chan, Kevin C. Wu, Ed X. Wong, David S. H. Biomed Res Int Research Article There is increasing use of the vitreous cavity as a reservoir for drug delivery. We study the intraocular migration and distribution of triamcinolone acetonide (TA) after injection into silicone oil tamponade agent during and after vitrectomy surgery ex vivo (pig eye) and in vitro (glass bottle). For ex vivo assessment, intraocular migration of TA was imaged using real-time FLASH MRI scans and high-resolution T2W imaging and the in vitro model was monitored continuously with a video camera. Results of the ex vivo experiment showed that the TA droplet sank to the interface of silicone oil and aqueous almost immediately after injection and remained inside the silicone oil bubble for as long as 16 minutes. The in vitro results showed that, after the shrinkage of the droplet, TA gradually precipitated leaving only a lump of whitish crystalline residue inside the droplet for about 100 minutes. TA then quickly broke the interface and dispersed into the underlying aqueous within 15 seconds, which may result in a momentary increase of local TA concentration in the aqueous portion and potentially toxic to the retina. Our study suggests that silicone oil may not be a good candidate as a drug reservoir for drugs like TA. Hindawi Publishing Corporation 2016 2016-07-14 /pmc/articles/PMC4963566/ /pubmed/27493959 http://dx.doi.org/10.1155/2016/5485467 Text en Copyright © 2016 Ma Da et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Da, Ma
Li, Kenneth K. W.
Chan, Kevin C.
Wu, Ed X.
Wong, David S. H.
Distribution of Triamcinolone Acetonide after Intravitreal Injection into Silicone Oil-Filled Eye
title Distribution of Triamcinolone Acetonide after Intravitreal Injection into Silicone Oil-Filled Eye
title_full Distribution of Triamcinolone Acetonide after Intravitreal Injection into Silicone Oil-Filled Eye
title_fullStr Distribution of Triamcinolone Acetonide after Intravitreal Injection into Silicone Oil-Filled Eye
title_full_unstemmed Distribution of Triamcinolone Acetonide after Intravitreal Injection into Silicone Oil-Filled Eye
title_short Distribution of Triamcinolone Acetonide after Intravitreal Injection into Silicone Oil-Filled Eye
title_sort distribution of triamcinolone acetonide after intravitreal injection into silicone oil-filled eye
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963566/
https://www.ncbi.nlm.nih.gov/pubmed/27493959
http://dx.doi.org/10.1155/2016/5485467
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