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Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ

The multifunctional Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes. However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear...

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Autores principales: Aji, Kaisaier, Maimaijiang, Munila, Aimaiti, Abudusaimi, Rexiati, Mulati, Azhati, Baihetiya, Tusong, Hamulati, Cui, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963582/
https://www.ncbi.nlm.nih.gov/pubmed/27493668
http://dx.doi.org/10.1155/2016/1267480
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author Aji, Kaisaier
Maimaijiang, Munila
Aimaiti, Abudusaimi
Rexiati, Mulati
Azhati, Baihetiya
Tusong, Hamulati
Cui, Lei
author_facet Aji, Kaisaier
Maimaijiang, Munila
Aimaiti, Abudusaimi
Rexiati, Mulati
Azhati, Baihetiya
Tusong, Hamulati
Cui, Lei
author_sort Aji, Kaisaier
collection PubMed
description The multifunctional Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes. However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear. In the present study, we detected γ isoform of CaMKII in differentiation of human adipose derived stem cells (hASCs) into SMCs that resulted from treatment with TGF-β1 and BMP4 in combination for 7 days. The results showed that CaMKIIγ increased gradually during differentiation of hASCs as determined by real-time PCR and western blot analysis. The siRNA-mediated knockdown of CaMKIIγ decreased the protein levels and transcriptional levels of smooth muscle contractile markers (a-SMA, SM22a, calponin, and SM-MHC), while CaMKIIγ overexpression increases the transcriptional and protein levels of smooth muscle contractile markers. These results suggested that γ isoform of CaMKII plays a significant role in smooth muscle differentiation of hASCs.
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spelling pubmed-49635822016-08-04 Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ Aji, Kaisaier Maimaijiang, Munila Aimaiti, Abudusaimi Rexiati, Mulati Azhati, Baihetiya Tusong, Hamulati Cui, Lei Stem Cells Int Research Article The multifunctional Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes. However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear. In the present study, we detected γ isoform of CaMKII in differentiation of human adipose derived stem cells (hASCs) into SMCs that resulted from treatment with TGF-β1 and BMP4 in combination for 7 days. The results showed that CaMKIIγ increased gradually during differentiation of hASCs as determined by real-time PCR and western blot analysis. The siRNA-mediated knockdown of CaMKIIγ decreased the protein levels and transcriptional levels of smooth muscle contractile markers (a-SMA, SM22a, calponin, and SM-MHC), while CaMKIIγ overexpression increases the transcriptional and protein levels of smooth muscle contractile markers. These results suggested that γ isoform of CaMKII plays a significant role in smooth muscle differentiation of hASCs. Hindawi Publishing Corporation 2016 2016-07-14 /pmc/articles/PMC4963582/ /pubmed/27493668 http://dx.doi.org/10.1155/2016/1267480 Text en Copyright © 2016 Kaisaier Aji et al. https://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Aji, Kaisaier
Maimaijiang, Munila
Aimaiti, Abudusaimi
Rexiati, Mulati
Azhati, Baihetiya
Tusong, Hamulati
Cui, Lei
Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ
title Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ
title_full Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ
title_fullStr Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ
title_full_unstemmed Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ
title_short Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ
title_sort differentiation of human adipose derived stem cells into smooth muscle cells is modulated by camkiiγ
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963582/
https://www.ncbi.nlm.nih.gov/pubmed/27493668
http://dx.doi.org/10.1155/2016/1267480
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