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Respiratory Effects of Sarafotoxins from the Venom of Different Atractaspis Genus Snake Species
Sarafotoxins (SRTX) are endothelin-like peptides extracted from the venom of snakes belonging to the Atractaspididae family. A recent in vivo study on anesthetized and ventilated animals showed that sarafotoxin-b (SRTX-b), extracted from the venom of Atractaspis engaddensis, decreases cardiac output...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963848/ https://www.ncbi.nlm.nih.gov/pubmed/27409637 http://dx.doi.org/10.3390/toxins8070215 |
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author | Malaquin, Stéphanie Bayat, Sam Abou Arab, Osama Mourier, Gilles Lorne, Emmanuel Kamel, Saïd Dupont, Hervé Ducancel, Frédéric Mahjoub, Yazine |
author_facet | Malaquin, Stéphanie Bayat, Sam Abou Arab, Osama Mourier, Gilles Lorne, Emmanuel Kamel, Saïd Dupont, Hervé Ducancel, Frédéric Mahjoub, Yazine |
author_sort | Malaquin, Stéphanie |
collection | PubMed |
description | Sarafotoxins (SRTX) are endothelin-like peptides extracted from the venom of snakes belonging to the Atractaspididae family. A recent in vivo study on anesthetized and ventilated animals showed that sarafotoxin-b (SRTX-b), extracted from the venom of Atractaspis engaddensis, decreases cardiac output by inducing left ventricular dysfunction while sarafotoxin-m (SRTX-m), extracted from the venom of Atractaspis microlepidota microlepidota, induces right ventricular dysfunction with increased airway pressure. The aim of the present experimental study was to compare the respiratory effects of SRTX-m and SRTX-b. Male Wistar rats were anesthetized, tracheotomized and mechanically ventilated. They received either a 1 LD(50) IV bolus of SRTX-b (n = 5) or 1 LD(50) of SRTX-m (n = 5). The low-frequency forced oscillation technique was used to measure respiratory impedance. Airway resistance (Raw), parenchymal damping (G) and elastance (H) were determined from impedance data, before and 5 min after SRTX injection. SRTX-m and SRTX-b injections induced acute hypoxia and metabolic acidosis with an increased anion gap. Both toxins markedly increased Raw, G and H, but with a much greater effect of SRTX-b on H, which may have been due to pulmonary edema in addition to bronchoconstriction. Therefore, despite their structural analogy, these two toxins exert different effects on respiratory function. These results emphasize the role of the C-terminal extension in the in vivo effect of these toxins. |
format | Online Article Text |
id | pubmed-4963848 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-49638482016-08-03 Respiratory Effects of Sarafotoxins from the Venom of Different Atractaspis Genus Snake Species Malaquin, Stéphanie Bayat, Sam Abou Arab, Osama Mourier, Gilles Lorne, Emmanuel Kamel, Saïd Dupont, Hervé Ducancel, Frédéric Mahjoub, Yazine Toxins (Basel) Article Sarafotoxins (SRTX) are endothelin-like peptides extracted from the venom of snakes belonging to the Atractaspididae family. A recent in vivo study on anesthetized and ventilated animals showed that sarafotoxin-b (SRTX-b), extracted from the venom of Atractaspis engaddensis, decreases cardiac output by inducing left ventricular dysfunction while sarafotoxin-m (SRTX-m), extracted from the venom of Atractaspis microlepidota microlepidota, induces right ventricular dysfunction with increased airway pressure. The aim of the present experimental study was to compare the respiratory effects of SRTX-m and SRTX-b. Male Wistar rats were anesthetized, tracheotomized and mechanically ventilated. They received either a 1 LD(50) IV bolus of SRTX-b (n = 5) or 1 LD(50) of SRTX-m (n = 5). The low-frequency forced oscillation technique was used to measure respiratory impedance. Airway resistance (Raw), parenchymal damping (G) and elastance (H) were determined from impedance data, before and 5 min after SRTX injection. SRTX-m and SRTX-b injections induced acute hypoxia and metabolic acidosis with an increased anion gap. Both toxins markedly increased Raw, G and H, but with a much greater effect of SRTX-b on H, which may have been due to pulmonary edema in addition to bronchoconstriction. Therefore, despite their structural analogy, these two toxins exert different effects on respiratory function. These results emphasize the role of the C-terminal extension in the in vivo effect of these toxins. MDPI 2016-07-11 /pmc/articles/PMC4963848/ /pubmed/27409637 http://dx.doi.org/10.3390/toxins8070215 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Malaquin, Stéphanie Bayat, Sam Abou Arab, Osama Mourier, Gilles Lorne, Emmanuel Kamel, Saïd Dupont, Hervé Ducancel, Frédéric Mahjoub, Yazine Respiratory Effects of Sarafotoxins from the Venom of Different Atractaspis Genus Snake Species |
title | Respiratory Effects of Sarafotoxins from the Venom of Different Atractaspis Genus Snake Species |
title_full | Respiratory Effects of Sarafotoxins from the Venom of Different Atractaspis Genus Snake Species |
title_fullStr | Respiratory Effects of Sarafotoxins from the Venom of Different Atractaspis Genus Snake Species |
title_full_unstemmed | Respiratory Effects of Sarafotoxins from the Venom of Different Atractaspis Genus Snake Species |
title_short | Respiratory Effects of Sarafotoxins from the Venom of Different Atractaspis Genus Snake Species |
title_sort | respiratory effects of sarafotoxins from the venom of different atractaspis genus snake species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4963848/ https://www.ncbi.nlm.nih.gov/pubmed/27409637 http://dx.doi.org/10.3390/toxins8070215 |
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