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Influence of phytochemicals in piper betle linn leaf extract on wound healing
BACKGROUND: Wound healing has being extensively investigated over the world. Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage, delaying in granulation tissue formation, reducing in angiogenesis and decreasing in collagen reorganization. These facts c...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964315/ https://www.ncbi.nlm.nih.gov/pubmed/27574669 http://dx.doi.org/10.1186/s41038-015-0023-7 |
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author | Lien, Le Thi Tho, Nguyen Thi Ha, Do Minh Hang, Pham Luong Nghia, Phan Tuan Thang, Nguyen Dinh |
author_facet | Lien, Le Thi Tho, Nguyen Thi Ha, Do Minh Hang, Pham Luong Nghia, Phan Tuan Thang, Nguyen Dinh |
author_sort | Lien, Le Thi |
collection | PubMed |
description | BACKGROUND: Wound healing has being extensively investigated over the world. Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage, delaying in granulation tissue formation, reducing in angiogenesis and decreasing in collagen reorganization. These facts consequently lead to chronic wound healing. Piper betle Linn (Betle) leaves have been folklore used as an ingredient of drugs for cutaneous wound treatment. However, the effect of betle leaf on wound healing is not yet well elucidated. In this study, we aimed to investigate the healing efficacy of methanol leaf extract of Piper betle Linn on proliferation of fibroblast NIH3T3 cells as well as full-thickness burn and excision wounds in swiss mice. METHODS: Scratch wound healing assays were conducted to examine the effects of betle leaf extract on healing activity of fibroblast cells. Burn and excision wounds on swiss mouse skins were created for investigating the wound healing progress caused by the betle leaf extract. Malondialdehyde (MDA) was also evaluated to examine the products of lipid hydroperoxide (LPO) under conditions of with or without betle leaf extract treatment. RESULTS: The results of this study showed that Piper betle Linn leaf extract in methanol increased proliferation of NIH3T3 cells and promoted wound healing in vitro and in vivo with both burn wound and excision wound models. In addition, this extract significant decreased level of malondialdehyde (MDA) in liver of treated-mice compared with that in non-treated mice. CONCLUSIONS: Our results suggest that Piper betle Linn can be used as an ingredient in developing natural origin drugs for treatment of cutaneous wounds. |
format | Online Article Text |
id | pubmed-4964315 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-49643152016-08-29 Influence of phytochemicals in piper betle linn leaf extract on wound healing Lien, Le Thi Tho, Nguyen Thi Ha, Do Minh Hang, Pham Luong Nghia, Phan Tuan Thang, Nguyen Dinh Burns Trauma Research Article BACKGROUND: Wound healing has being extensively investigated over the world. Healing impairment is caused by many reasons including increasing of free-radicals-mediated damage, delaying in granulation tissue formation, reducing in angiogenesis and decreasing in collagen reorganization. These facts consequently lead to chronic wound healing. Piper betle Linn (Betle) leaves have been folklore used as an ingredient of drugs for cutaneous wound treatment. However, the effect of betle leaf on wound healing is not yet well elucidated. In this study, we aimed to investigate the healing efficacy of methanol leaf extract of Piper betle Linn on proliferation of fibroblast NIH3T3 cells as well as full-thickness burn and excision wounds in swiss mice. METHODS: Scratch wound healing assays were conducted to examine the effects of betle leaf extract on healing activity of fibroblast cells. Burn and excision wounds on swiss mouse skins were created for investigating the wound healing progress caused by the betle leaf extract. Malondialdehyde (MDA) was also evaluated to examine the products of lipid hydroperoxide (LPO) under conditions of with or without betle leaf extract treatment. RESULTS: The results of this study showed that Piper betle Linn leaf extract in methanol increased proliferation of NIH3T3 cells and promoted wound healing in vitro and in vivo with both burn wound and excision wound models. In addition, this extract significant decreased level of malondialdehyde (MDA) in liver of treated-mice compared with that in non-treated mice. CONCLUSIONS: Our results suggest that Piper betle Linn can be used as an ingredient in developing natural origin drugs for treatment of cutaneous wounds. BioMed Central 2015-12-01 /pmc/articles/PMC4964315/ /pubmed/27574669 http://dx.doi.org/10.1186/s41038-015-0023-7 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Article Lien, Le Thi Tho, Nguyen Thi Ha, Do Minh Hang, Pham Luong Nghia, Phan Tuan Thang, Nguyen Dinh Influence of phytochemicals in piper betle linn leaf extract on wound healing |
title | Influence of phytochemicals in piper betle linn leaf extract on wound healing |
title_full | Influence of phytochemicals in piper betle linn leaf extract on wound healing |
title_fullStr | Influence of phytochemicals in piper betle linn leaf extract on wound healing |
title_full_unstemmed | Influence of phytochemicals in piper betle linn leaf extract on wound healing |
title_short | Influence of phytochemicals in piper betle linn leaf extract on wound healing |
title_sort | influence of phytochemicals in piper betle linn leaf extract on wound healing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964315/ https://www.ncbi.nlm.nih.gov/pubmed/27574669 http://dx.doi.org/10.1186/s41038-015-0023-7 |
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