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The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations

Due to the polyanionic nature of RNA, the principles of charge neutralization and electrostatic condensation require that cations help to overcome the repulsive forces in order for RNA to adopt a three-dimensional structure. A precise structural knowledge of RNA-metal ion interactions is crucial to...

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Autores principales: Schaffer, Michelle F., Peng, Guanya, Spingler, Bernhard, Schnabl, Joachim, Wang, Meitian, Olieric, Vincent, Sigel, Roland K. O.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964368/
https://www.ncbi.nlm.nih.gov/pubmed/27355942
http://dx.doi.org/10.3390/ijms17070988
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author Schaffer, Michelle F.
Peng, Guanya
Spingler, Bernhard
Schnabl, Joachim
Wang, Meitian
Olieric, Vincent
Sigel, Roland K. O.
author_facet Schaffer, Michelle F.
Peng, Guanya
Spingler, Bernhard
Schnabl, Joachim
Wang, Meitian
Olieric, Vincent
Sigel, Roland K. O.
author_sort Schaffer, Michelle F.
collection PubMed
description Due to the polyanionic nature of RNA, the principles of charge neutralization and electrostatic condensation require that cations help to overcome the repulsive forces in order for RNA to adopt a three-dimensional structure. A precise structural knowledge of RNA-metal ion interactions is crucial to understand the mechanism of metal ions in the catalytic or regulatory activity of RNA. We solved the crystal structure of an octameric RNA duplex in the presence of the di- and trivalent metal ions Ca(2+), Mn(2+), Co(2+), Cu(2+), Sr(2+), and Tb(3+). The detailed investigation reveals a unique innersphere interaction to uracil and extends the knowledge of the influence of metal ions for conformational changes in RNA structure. Furthermore, we could demonstrate that an accurate localization of the metal ions in the X-ray structures require the consideration of several crystallographic and geometrical parameters as well as the anomalous difference map.
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spelling pubmed-49643682016-08-03 The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations Schaffer, Michelle F. Peng, Guanya Spingler, Bernhard Schnabl, Joachim Wang, Meitian Olieric, Vincent Sigel, Roland K. O. Int J Mol Sci Article Due to the polyanionic nature of RNA, the principles of charge neutralization and electrostatic condensation require that cations help to overcome the repulsive forces in order for RNA to adopt a three-dimensional structure. A precise structural knowledge of RNA-metal ion interactions is crucial to understand the mechanism of metal ions in the catalytic or regulatory activity of RNA. We solved the crystal structure of an octameric RNA duplex in the presence of the di- and trivalent metal ions Ca(2+), Mn(2+), Co(2+), Cu(2+), Sr(2+), and Tb(3+). The detailed investigation reveals a unique innersphere interaction to uracil and extends the knowledge of the influence of metal ions for conformational changes in RNA structure. Furthermore, we could demonstrate that an accurate localization of the metal ions in the X-ray structures require the consideration of several crystallographic and geometrical parameters as well as the anomalous difference map. MDPI 2016-06-27 /pmc/articles/PMC4964368/ /pubmed/27355942 http://dx.doi.org/10.3390/ijms17070988 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schaffer, Michelle F.
Peng, Guanya
Spingler, Bernhard
Schnabl, Joachim
Wang, Meitian
Olieric, Vincent
Sigel, Roland K. O.
The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations
title The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations
title_full The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations
title_fullStr The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations
title_full_unstemmed The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations
title_short The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations
title_sort x-ray structures of six octameric rna duplexes in the presence of different di- and trivalent cations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964368/
https://www.ncbi.nlm.nih.gov/pubmed/27355942
http://dx.doi.org/10.3390/ijms17070988
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