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The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations
Due to the polyanionic nature of RNA, the principles of charge neutralization and electrostatic condensation require that cations help to overcome the repulsive forces in order for RNA to adopt a three-dimensional structure. A precise structural knowledge of RNA-metal ion interactions is crucial to...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964368/ https://www.ncbi.nlm.nih.gov/pubmed/27355942 http://dx.doi.org/10.3390/ijms17070988 |
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author | Schaffer, Michelle F. Peng, Guanya Spingler, Bernhard Schnabl, Joachim Wang, Meitian Olieric, Vincent Sigel, Roland K. O. |
author_facet | Schaffer, Michelle F. Peng, Guanya Spingler, Bernhard Schnabl, Joachim Wang, Meitian Olieric, Vincent Sigel, Roland K. O. |
author_sort | Schaffer, Michelle F. |
collection | PubMed |
description | Due to the polyanionic nature of RNA, the principles of charge neutralization and electrostatic condensation require that cations help to overcome the repulsive forces in order for RNA to adopt a three-dimensional structure. A precise structural knowledge of RNA-metal ion interactions is crucial to understand the mechanism of metal ions in the catalytic or regulatory activity of RNA. We solved the crystal structure of an octameric RNA duplex in the presence of the di- and trivalent metal ions Ca(2+), Mn(2+), Co(2+), Cu(2+), Sr(2+), and Tb(3+). The detailed investigation reveals a unique innersphere interaction to uracil and extends the knowledge of the influence of metal ions for conformational changes in RNA structure. Furthermore, we could demonstrate that an accurate localization of the metal ions in the X-ray structures require the consideration of several crystallographic and geometrical parameters as well as the anomalous difference map. |
format | Online Article Text |
id | pubmed-4964368 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-49643682016-08-03 The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations Schaffer, Michelle F. Peng, Guanya Spingler, Bernhard Schnabl, Joachim Wang, Meitian Olieric, Vincent Sigel, Roland K. O. Int J Mol Sci Article Due to the polyanionic nature of RNA, the principles of charge neutralization and electrostatic condensation require that cations help to overcome the repulsive forces in order for RNA to adopt a three-dimensional structure. A precise structural knowledge of RNA-metal ion interactions is crucial to understand the mechanism of metal ions in the catalytic or regulatory activity of RNA. We solved the crystal structure of an octameric RNA duplex in the presence of the di- and trivalent metal ions Ca(2+), Mn(2+), Co(2+), Cu(2+), Sr(2+), and Tb(3+). The detailed investigation reveals a unique innersphere interaction to uracil and extends the knowledge of the influence of metal ions for conformational changes in RNA structure. Furthermore, we could demonstrate that an accurate localization of the metal ions in the X-ray structures require the consideration of several crystallographic and geometrical parameters as well as the anomalous difference map. MDPI 2016-06-27 /pmc/articles/PMC4964368/ /pubmed/27355942 http://dx.doi.org/10.3390/ijms17070988 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Schaffer, Michelle F. Peng, Guanya Spingler, Bernhard Schnabl, Joachim Wang, Meitian Olieric, Vincent Sigel, Roland K. O. The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations |
title | The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations |
title_full | The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations |
title_fullStr | The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations |
title_full_unstemmed | The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations |
title_short | The X-ray Structures of Six Octameric RNA Duplexes in the Presence of Different Di- and Trivalent Cations |
title_sort | x-ray structures of six octameric rna duplexes in the presence of different di- and trivalent cations |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964368/ https://www.ncbi.nlm.nih.gov/pubmed/27355942 http://dx.doi.org/10.3390/ijms17070988 |
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