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In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria

Electron microscopy was used to test whether or not (a) in statu nascendi synthesized, and in situ measured, nanoparticle size does not differ significantly from the size of nanoparticles after their purification; and (b) the generation of selenium is detrimental to the bacterial strains that produc...

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Autores principales: Nagy, Gabor, Pinczes, Gyula, Pinter, Gabor, Pocsi, Istvan, Prokisch, Jozsef, Banfalvi, Gaspar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964423/
https://www.ncbi.nlm.nih.gov/pubmed/27376279
http://dx.doi.org/10.3390/ijms17071047
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author Nagy, Gabor
Pinczes, Gyula
Pinter, Gabor
Pocsi, Istvan
Prokisch, Jozsef
Banfalvi, Gaspar
author_facet Nagy, Gabor
Pinczes, Gyula
Pinter, Gabor
Pocsi, Istvan
Prokisch, Jozsef
Banfalvi, Gaspar
author_sort Nagy, Gabor
collection PubMed
description Electron microscopy was used to test whether or not (a) in statu nascendi synthesized, and in situ measured, nanoparticle size does not differ significantly from the size of nanoparticles after their purification; and (b) the generation of selenium is detrimental to the bacterial strains that produce them. Elemental nano-sized selenium produced by probiotic latic acid bacteria was used as a lactomicroselenium (lactomicroSel) inhibitor of cell growth in the presence of lactomicroSel, and was followed by time-lapse microscopy. The size of lactomicroSel produced by probiotic bacteria was measured in situ and after isolation and purification. For these measurements the TESLA BS 540 transmission electron microscope was converted from analog (aTEM) to digital processing (dTEM), and further to remote-access internet electron microscopy (iTEM). Lactobacillus acidophilus produced fewer, but larger, lactomicroSel nanoparticles (200–350 nm) than Lactobacillus casei (L. casei), which generated many, smaller lactomicroSel particles (85–200 nm) and grains as a cloudy, less electrodense material. Streptococcus thermophilus cells generated selenoparticles (60–280 nm) in a suicidic manner. The size determined in situ in lactic acid bacteria was significantly lower than those measured by scanning electron microscopy after the isolation of lactomicroSel particles obtained from lactobacilli (100–500 nm), but higher relative to those isolated from Streptococcus thermopilus (50–100 nm). These differences indicate that smaller lactomicroSel particles could be more toxic to the producing bacteria themselves and discrepancies in size could have implications with respect to the applications of selenium nanoparticles as prebiotics.
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spelling pubmed-49644232016-08-03 In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria Nagy, Gabor Pinczes, Gyula Pinter, Gabor Pocsi, Istvan Prokisch, Jozsef Banfalvi, Gaspar Int J Mol Sci Communication Electron microscopy was used to test whether or not (a) in statu nascendi synthesized, and in situ measured, nanoparticle size does not differ significantly from the size of nanoparticles after their purification; and (b) the generation of selenium is detrimental to the bacterial strains that produce them. Elemental nano-sized selenium produced by probiotic latic acid bacteria was used as a lactomicroselenium (lactomicroSel) inhibitor of cell growth in the presence of lactomicroSel, and was followed by time-lapse microscopy. The size of lactomicroSel produced by probiotic bacteria was measured in situ and after isolation and purification. For these measurements the TESLA BS 540 transmission electron microscope was converted from analog (aTEM) to digital processing (dTEM), and further to remote-access internet electron microscopy (iTEM). Lactobacillus acidophilus produced fewer, but larger, lactomicroSel nanoparticles (200–350 nm) than Lactobacillus casei (L. casei), which generated many, smaller lactomicroSel particles (85–200 nm) and grains as a cloudy, less electrodense material. Streptococcus thermophilus cells generated selenoparticles (60–280 nm) in a suicidic manner. The size determined in situ in lactic acid bacteria was significantly lower than those measured by scanning electron microscopy after the isolation of lactomicroSel particles obtained from lactobacilli (100–500 nm), but higher relative to those isolated from Streptococcus thermopilus (50–100 nm). These differences indicate that smaller lactomicroSel particles could be more toxic to the producing bacteria themselves and discrepancies in size could have implications with respect to the applications of selenium nanoparticles as prebiotics. MDPI 2016-06-30 /pmc/articles/PMC4964423/ /pubmed/27376279 http://dx.doi.org/10.3390/ijms17071047 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Nagy, Gabor
Pinczes, Gyula
Pinter, Gabor
Pocsi, Istvan
Prokisch, Jozsef
Banfalvi, Gaspar
In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria
title In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria
title_full In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria
title_fullStr In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria
title_full_unstemmed In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria
title_short In Situ Electron Microscopy of Lactomicroselenium Particles in Probiotic Bacteria
title_sort in situ electron microscopy of lactomicroselenium particles in probiotic bacteria
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964423/
https://www.ncbi.nlm.nih.gov/pubmed/27376279
http://dx.doi.org/10.3390/ijms17071047
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