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Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana

Piriformospora indica is a root-colonizing fungus, which interacts with a variety of plants including Arabidopsis thaliana. This interaction has been considered as mutualistic leading to growth promotion of the host. So far, only indolic glucosinolates and phytohormones have been identified as key p...

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Autores principales: Strehmel, Nadine, Mönchgesang, Susann, Herklotz, Siska, Krüger, Sylvia, Ziegler, Jörg, Scheel, Dierk
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964467/
https://www.ncbi.nlm.nih.gov/pubmed/27399695
http://dx.doi.org/10.3390/ijms17071091
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author Strehmel, Nadine
Mönchgesang, Susann
Herklotz, Siska
Krüger, Sylvia
Ziegler, Jörg
Scheel, Dierk
author_facet Strehmel, Nadine
Mönchgesang, Susann
Herklotz, Siska
Krüger, Sylvia
Ziegler, Jörg
Scheel, Dierk
author_sort Strehmel, Nadine
collection PubMed
description Piriformospora indica is a root-colonizing fungus, which interacts with a variety of plants including Arabidopsis thaliana. This interaction has been considered as mutualistic leading to growth promotion of the host. So far, only indolic glucosinolates and phytohormones have been identified as key players. In a comprehensive non-targeted metabolite profiling study, we analyzed Arabidopsis thaliana’s roots, root exudates, and leaves of inoculated and non-inoculated plants by ultra performance liquid chromatography/electrospray ionization quadrupole-time-of-flight mass spectrometry (UPLC/(ESI)-QTOFMS) and gas chromatography/electron ionization quadrupole mass spectrometry (GC/EI-QMS), and identified further biomarkers. Among them, the concentration of nucleosides, dipeptides, oligolignols, and glucosinolate degradation products was affected in the exudates. In the root profiles, nearly all metabolite levels increased upon co-cultivation, like carbohydrates, organic acids, amino acids, glucosinolates, oligolignols, and flavonoids. In the leaf profiles, we detected by far less significant changes. We only observed an increased concentration of organic acids, carbohydrates, ascorbate, glucosinolates and hydroxycinnamic acids, and a decreased concentration of nitrogen-rich amino acids in inoculated plants. These findings contribute to the understanding of symbiotic interactions between plant roots and fungi of the order of Sebacinales and are a valid source for follow-up mechanistic studies, because these symbioses are particular and clearly different from interactions of roots with mycorrhizal fungi or dark septate endophytes
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spelling pubmed-49644672016-08-03 Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana Strehmel, Nadine Mönchgesang, Susann Herklotz, Siska Krüger, Sylvia Ziegler, Jörg Scheel, Dierk Int J Mol Sci Article Piriformospora indica is a root-colonizing fungus, which interacts with a variety of plants including Arabidopsis thaliana. This interaction has been considered as mutualistic leading to growth promotion of the host. So far, only indolic glucosinolates and phytohormones have been identified as key players. In a comprehensive non-targeted metabolite profiling study, we analyzed Arabidopsis thaliana’s roots, root exudates, and leaves of inoculated and non-inoculated plants by ultra performance liquid chromatography/electrospray ionization quadrupole-time-of-flight mass spectrometry (UPLC/(ESI)-QTOFMS) and gas chromatography/electron ionization quadrupole mass spectrometry (GC/EI-QMS), and identified further biomarkers. Among them, the concentration of nucleosides, dipeptides, oligolignols, and glucosinolate degradation products was affected in the exudates. In the root profiles, nearly all metabolite levels increased upon co-cultivation, like carbohydrates, organic acids, amino acids, glucosinolates, oligolignols, and flavonoids. In the leaf profiles, we detected by far less significant changes. We only observed an increased concentration of organic acids, carbohydrates, ascorbate, glucosinolates and hydroxycinnamic acids, and a decreased concentration of nitrogen-rich amino acids in inoculated plants. These findings contribute to the understanding of symbiotic interactions between plant roots and fungi of the order of Sebacinales and are a valid source for follow-up mechanistic studies, because these symbioses are particular and clearly different from interactions of roots with mycorrhizal fungi or dark septate endophytes MDPI 2016-07-08 /pmc/articles/PMC4964467/ /pubmed/27399695 http://dx.doi.org/10.3390/ijms17071091 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Strehmel, Nadine
Mönchgesang, Susann
Herklotz, Siska
Krüger, Sylvia
Ziegler, Jörg
Scheel, Dierk
Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana
title Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana
title_full Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana
title_fullStr Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana
title_full_unstemmed Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana
title_short Piriformospora indica Stimulates Root Metabolism of Arabidopsis thaliana
title_sort piriformospora indica stimulates root metabolism of arabidopsis thaliana
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964467/
https://www.ncbi.nlm.nih.gov/pubmed/27399695
http://dx.doi.org/10.3390/ijms17071091
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