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High Morphologic Plasticity of Microglia/Macrophages Following Experimental Intracerebral Hemorrhage in Rats
As current efforts have limited effects on the clinical outcome of intracerebral hemorrhage (ICH), the mechanisms including microglia/macrophages that involved inflammation need further investigation. Here, 0.4 units of collagenase VII were injected into the left caudate putamen (CPu) to duplicate I...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964551/ https://www.ncbi.nlm.nih.gov/pubmed/27455236 http://dx.doi.org/10.3390/ijms17071181 |
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author | Yang, Shu-Sheng Lin, Li Liu, Yue Wang, Jie Chu, Jiang Zhang, Teng Ning, Lin-Na Shi, Yan Fang, Ying-Yan Zeng, Peng Wang, Jian-Zhi Qiu, Ming-Yi Tian, Qing |
author_facet | Yang, Shu-Sheng Lin, Li Liu, Yue Wang, Jie Chu, Jiang Zhang, Teng Ning, Lin-Na Shi, Yan Fang, Ying-Yan Zeng, Peng Wang, Jian-Zhi Qiu, Ming-Yi Tian, Qing |
author_sort | Yang, Shu-Sheng |
collection | PubMed |
description | As current efforts have limited effects on the clinical outcome of intracerebral hemorrhage (ICH), the mechanisms including microglia/macrophages that involved inflammation need further investigation. Here, 0.4 units of collagenase VII were injected into the left caudate putamen (CPu) to duplicate ICH rat models. In the brains of ICH rats, microglia/macrophages, the nearest cells to the hemorrhagic center, were observed as ameboid and Prussian-blue positive. Furthermore, the ameboid microglia/macrophages were differentiation (CD) 68 and interleukin-1β (IL-1β) positive, and neither CD206 nor chitinase3-like 3 (Ym1) positive, suggesting their strong abilities of phagocytosis and secretion of IL-1β. According to the distance to the hemorrhagic center, we selected four areas—I, II, III, and IV—to analyze the morphology of microglia/macrophages. The processes decreased successively from region I to region IV. Microglia/macrophages in region IV had no processes. The processes in region I were radially distributed, however, they showed obvious directivity towards the hemorrhagic center in regions II and III. Region III had the largest density of compactly arrayed microglia/macrophages. All these in vivo results present the high morphologic plasticity of microglia/macrophages and their functions in the pathogenesis of ICHs. |
format | Online Article Text |
id | pubmed-4964551 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-49645512016-08-03 High Morphologic Plasticity of Microglia/Macrophages Following Experimental Intracerebral Hemorrhage in Rats Yang, Shu-Sheng Lin, Li Liu, Yue Wang, Jie Chu, Jiang Zhang, Teng Ning, Lin-Na Shi, Yan Fang, Ying-Yan Zeng, Peng Wang, Jian-Zhi Qiu, Ming-Yi Tian, Qing Int J Mol Sci Article As current efforts have limited effects on the clinical outcome of intracerebral hemorrhage (ICH), the mechanisms including microglia/macrophages that involved inflammation need further investigation. Here, 0.4 units of collagenase VII were injected into the left caudate putamen (CPu) to duplicate ICH rat models. In the brains of ICH rats, microglia/macrophages, the nearest cells to the hemorrhagic center, were observed as ameboid and Prussian-blue positive. Furthermore, the ameboid microglia/macrophages were differentiation (CD) 68 and interleukin-1β (IL-1β) positive, and neither CD206 nor chitinase3-like 3 (Ym1) positive, suggesting their strong abilities of phagocytosis and secretion of IL-1β. According to the distance to the hemorrhagic center, we selected four areas—I, II, III, and IV—to analyze the morphology of microglia/macrophages. The processes decreased successively from region I to region IV. Microglia/macrophages in region IV had no processes. The processes in region I were radially distributed, however, they showed obvious directivity towards the hemorrhagic center in regions II and III. Region III had the largest density of compactly arrayed microglia/macrophages. All these in vivo results present the high morphologic plasticity of microglia/macrophages and their functions in the pathogenesis of ICHs. MDPI 2016-07-21 /pmc/articles/PMC4964551/ /pubmed/27455236 http://dx.doi.org/10.3390/ijms17071181 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Shu-Sheng Lin, Li Liu, Yue Wang, Jie Chu, Jiang Zhang, Teng Ning, Lin-Na Shi, Yan Fang, Ying-Yan Zeng, Peng Wang, Jian-Zhi Qiu, Ming-Yi Tian, Qing High Morphologic Plasticity of Microglia/Macrophages Following Experimental Intracerebral Hemorrhage in Rats |
title | High Morphologic Plasticity of Microglia/Macrophages Following Experimental Intracerebral Hemorrhage in Rats |
title_full | High Morphologic Plasticity of Microglia/Macrophages Following Experimental Intracerebral Hemorrhage in Rats |
title_fullStr | High Morphologic Plasticity of Microglia/Macrophages Following Experimental Intracerebral Hemorrhage in Rats |
title_full_unstemmed | High Morphologic Plasticity of Microglia/Macrophages Following Experimental Intracerebral Hemorrhage in Rats |
title_short | High Morphologic Plasticity of Microglia/Macrophages Following Experimental Intracerebral Hemorrhage in Rats |
title_sort | high morphologic plasticity of microglia/macrophages following experimental intracerebral hemorrhage in rats |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964551/ https://www.ncbi.nlm.nih.gov/pubmed/27455236 http://dx.doi.org/10.3390/ijms17071181 |
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