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Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury

This study investigated the neuroprotective effect of salvianolic acids (SA) against ischemia/reperfusion (I/R) injury, and explored whether the neuroprotection was dependent on mitochondrial connexin43 (mtCx43) via the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. In vitro, we...

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Autores principales: Hou, Shuai, Zhao, Ming-Ming, Shen, Ping-Ping, Liu, Xiu-Ping, Sun, Yuan, Feng, Jia-Chun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964559/
https://www.ncbi.nlm.nih.gov/pubmed/27455249
http://dx.doi.org/10.3390/ijms17071190
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author Hou, Shuai
Zhao, Ming-Ming
Shen, Ping-Ping
Liu, Xiu-Ping
Sun, Yuan
Feng, Jia-Chun
author_facet Hou, Shuai
Zhao, Ming-Ming
Shen, Ping-Ping
Liu, Xiu-Ping
Sun, Yuan
Feng, Jia-Chun
author_sort Hou, Shuai
collection PubMed
description This study investigated the neuroprotective effect of salvianolic acids (SA) against ischemia/reperfusion (I/R) injury, and explored whether the neuroprotection was dependent on mitochondrial connexin43 (mtCx43) via the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. In vitro, we measured astrocyte apoptosis, mitochondrial membrane potential, and also evaluated the morphology of astrocyte mitochondria with transmission electron microscopy. In vivo, we determined the cerebral infarction volume and measured superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Additionally, mtCx43, p-mtCx43, AKT, and p-AKT levels were determined. In vitro, we found that I/R injury induced apoptosis, decreased cell mitochondrial membrane potential (MMP), and damaged mitochondrial morphology in astrocytes. In vivo, we found that I/R injury resulted in a large cerebral infarction, decreased SOD activity, and increased MDA expression. Additionally, I/R injury reduced both the p-mtCx43/mtCx43 and p-AKT/AKT ratios. We reported that both in vivo and in vitro, SA ameliorated the detrimental outcomes of the I/R. Interestingly, co-administering an inhibitor of the PI3K/AKT pathway blunted the effects of SA. SA represents a potential treatment option for cerebral infarction by up-regulating mtCx43 through the PI3K/AKT pathway.
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spelling pubmed-49645592016-08-03 Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury Hou, Shuai Zhao, Ming-Ming Shen, Ping-Ping Liu, Xiu-Ping Sun, Yuan Feng, Jia-Chun Int J Mol Sci Article This study investigated the neuroprotective effect of salvianolic acids (SA) against ischemia/reperfusion (I/R) injury, and explored whether the neuroprotection was dependent on mitochondrial connexin43 (mtCx43) via the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. In vitro, we measured astrocyte apoptosis, mitochondrial membrane potential, and also evaluated the morphology of astrocyte mitochondria with transmission electron microscopy. In vivo, we determined the cerebral infarction volume and measured superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Additionally, mtCx43, p-mtCx43, AKT, and p-AKT levels were determined. In vitro, we found that I/R injury induced apoptosis, decreased cell mitochondrial membrane potential (MMP), and damaged mitochondrial morphology in astrocytes. In vivo, we found that I/R injury resulted in a large cerebral infarction, decreased SOD activity, and increased MDA expression. Additionally, I/R injury reduced both the p-mtCx43/mtCx43 and p-AKT/AKT ratios. We reported that both in vivo and in vitro, SA ameliorated the detrimental outcomes of the I/R. Interestingly, co-administering an inhibitor of the PI3K/AKT pathway blunted the effects of SA. SA represents a potential treatment option for cerebral infarction by up-regulating mtCx43 through the PI3K/AKT pathway. MDPI 2016-07-22 /pmc/articles/PMC4964559/ /pubmed/27455249 http://dx.doi.org/10.3390/ijms17071190 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hou, Shuai
Zhao, Ming-Ming
Shen, Ping-Ping
Liu, Xiu-Ping
Sun, Yuan
Feng, Jia-Chun
Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury
title Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury
title_full Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury
title_fullStr Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury
title_full_unstemmed Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury
title_short Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury
title_sort neuroprotective effect of salvianolic acids against cerebral ischemia/reperfusion injury
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964559/
https://www.ncbi.nlm.nih.gov/pubmed/27455249
http://dx.doi.org/10.3390/ijms17071190
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