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Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury
This study investigated the neuroprotective effect of salvianolic acids (SA) against ischemia/reperfusion (I/R) injury, and explored whether the neuroprotection was dependent on mitochondrial connexin43 (mtCx43) via the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. In vitro, we...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964559/ https://www.ncbi.nlm.nih.gov/pubmed/27455249 http://dx.doi.org/10.3390/ijms17071190 |
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author | Hou, Shuai Zhao, Ming-Ming Shen, Ping-Ping Liu, Xiu-Ping Sun, Yuan Feng, Jia-Chun |
author_facet | Hou, Shuai Zhao, Ming-Ming Shen, Ping-Ping Liu, Xiu-Ping Sun, Yuan Feng, Jia-Chun |
author_sort | Hou, Shuai |
collection | PubMed |
description | This study investigated the neuroprotective effect of salvianolic acids (SA) against ischemia/reperfusion (I/R) injury, and explored whether the neuroprotection was dependent on mitochondrial connexin43 (mtCx43) via the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. In vitro, we measured astrocyte apoptosis, mitochondrial membrane potential, and also evaluated the morphology of astrocyte mitochondria with transmission electron microscopy. In vivo, we determined the cerebral infarction volume and measured superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Additionally, mtCx43, p-mtCx43, AKT, and p-AKT levels were determined. In vitro, we found that I/R injury induced apoptosis, decreased cell mitochondrial membrane potential (MMP), and damaged mitochondrial morphology in astrocytes. In vivo, we found that I/R injury resulted in a large cerebral infarction, decreased SOD activity, and increased MDA expression. Additionally, I/R injury reduced both the p-mtCx43/mtCx43 and p-AKT/AKT ratios. We reported that both in vivo and in vitro, SA ameliorated the detrimental outcomes of the I/R. Interestingly, co-administering an inhibitor of the PI3K/AKT pathway blunted the effects of SA. SA represents a potential treatment option for cerebral infarction by up-regulating mtCx43 through the PI3K/AKT pathway. |
format | Online Article Text |
id | pubmed-4964559 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-49645592016-08-03 Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury Hou, Shuai Zhao, Ming-Ming Shen, Ping-Ping Liu, Xiu-Ping Sun, Yuan Feng, Jia-Chun Int J Mol Sci Article This study investigated the neuroprotective effect of salvianolic acids (SA) against ischemia/reperfusion (I/R) injury, and explored whether the neuroprotection was dependent on mitochondrial connexin43 (mtCx43) via the phosphatidylinositol 3-kinase/protein kinase B (PI3K/AKT) pathway. In vitro, we measured astrocyte apoptosis, mitochondrial membrane potential, and also evaluated the morphology of astrocyte mitochondria with transmission electron microscopy. In vivo, we determined the cerebral infarction volume and measured superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Additionally, mtCx43, p-mtCx43, AKT, and p-AKT levels were determined. In vitro, we found that I/R injury induced apoptosis, decreased cell mitochondrial membrane potential (MMP), and damaged mitochondrial morphology in astrocytes. In vivo, we found that I/R injury resulted in a large cerebral infarction, decreased SOD activity, and increased MDA expression. Additionally, I/R injury reduced both the p-mtCx43/mtCx43 and p-AKT/AKT ratios. We reported that both in vivo and in vitro, SA ameliorated the detrimental outcomes of the I/R. Interestingly, co-administering an inhibitor of the PI3K/AKT pathway blunted the effects of SA. SA represents a potential treatment option for cerebral infarction by up-regulating mtCx43 through the PI3K/AKT pathway. MDPI 2016-07-22 /pmc/articles/PMC4964559/ /pubmed/27455249 http://dx.doi.org/10.3390/ijms17071190 Text en © 2016 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC-BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hou, Shuai Zhao, Ming-Ming Shen, Ping-Ping Liu, Xiu-Ping Sun, Yuan Feng, Jia-Chun Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury |
title | Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury |
title_full | Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury |
title_fullStr | Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury |
title_full_unstemmed | Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury |
title_short | Neuroprotective Effect of Salvianolic Acids against Cerebral Ischemia/Reperfusion Injury |
title_sort | neuroprotective effect of salvianolic acids against cerebral ischemia/reperfusion injury |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964559/ https://www.ncbi.nlm.nih.gov/pubmed/27455249 http://dx.doi.org/10.3390/ijms17071190 |
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