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Discovery of midgut genes for the RNA interference control of corn rootworm

RNA interference (RNAi) is a promising new technology for corn rootworm control. This paper presents the discovery of new gene targets - dvssj1 and dvssj2, in western corn rootworm (WCR). Dvssj1 and dvssj2 are orthologs of the Drosophila genes snakeskin (ssk) and mesh, respectively. These genes enco...

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Autores principales: Hu, Xu, Richtman, Nina M., Zhao, Jian-Zhou, Duncan, Keith E., Niu, Xiping, Procyk, Lisa A., Oneal, Meghan A., Kernodle, Bliss M., Steimel, Joseph P., Crane, Virginia C., Sandahl, Gary, Ritland, Julie L., Howard, Richard J., Presnail, James K., Lu, Albert L., Wu, Gusui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964579/
https://www.ncbi.nlm.nih.gov/pubmed/27464714
http://dx.doi.org/10.1038/srep30542
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author Hu, Xu
Richtman, Nina M.
Zhao, Jian-Zhou
Duncan, Keith E.
Niu, Xiping
Procyk, Lisa A.
Oneal, Meghan A.
Kernodle, Bliss M.
Steimel, Joseph P.
Crane, Virginia C.
Sandahl, Gary
Ritland, Julie L.
Howard, Richard J.
Presnail, James K.
Lu, Albert L.
Wu, Gusui
author_facet Hu, Xu
Richtman, Nina M.
Zhao, Jian-Zhou
Duncan, Keith E.
Niu, Xiping
Procyk, Lisa A.
Oneal, Meghan A.
Kernodle, Bliss M.
Steimel, Joseph P.
Crane, Virginia C.
Sandahl, Gary
Ritland, Julie L.
Howard, Richard J.
Presnail, James K.
Lu, Albert L.
Wu, Gusui
author_sort Hu, Xu
collection PubMed
description RNA interference (RNAi) is a promising new technology for corn rootworm control. This paper presents the discovery of new gene targets - dvssj1 and dvssj2, in western corn rootworm (WCR). Dvssj1 and dvssj2 are orthologs of the Drosophila genes snakeskin (ssk) and mesh, respectively. These genes encode membrane proteins associated with smooth septate junctions (SSJ) which are required for intestinal barrier function. Based on bioinformatics analysis, dvssj1 appears to be an arthropod-specific gene. Diet based insect feeding assays using double-stranded RNA (dsRNA) targeting dvssj1 and dvssj2 demonstrate targeted mRNA suppression, larval growth inhibition, and mortality. In RNAi treated WCR, injury to the midgut was manifested by “blebbing” of the midgut epithelium into the gut lumen. Ultrastructural examination of midgut epithelial cells revealed apoptosis and regenerative activities. Transgenic plants expressing dsRNA targeting dvssj1 show insecticidal activity and significant plant protection from WCR damage. The data indicate that dvssj1 and dvssj2 are effective gene targets for the control of WCR using RNAi technology, by apparent suppression of production of their respective smooth septate junction membrane proteins located within the intestinal lining, leading to growth inhibition and mortality.
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spelling pubmed-49645792016-08-08 Discovery of midgut genes for the RNA interference control of corn rootworm Hu, Xu Richtman, Nina M. Zhao, Jian-Zhou Duncan, Keith E. Niu, Xiping Procyk, Lisa A. Oneal, Meghan A. Kernodle, Bliss M. Steimel, Joseph P. Crane, Virginia C. Sandahl, Gary Ritland, Julie L. Howard, Richard J. Presnail, James K. Lu, Albert L. Wu, Gusui Sci Rep Article RNA interference (RNAi) is a promising new technology for corn rootworm control. This paper presents the discovery of new gene targets - dvssj1 and dvssj2, in western corn rootworm (WCR). Dvssj1 and dvssj2 are orthologs of the Drosophila genes snakeskin (ssk) and mesh, respectively. These genes encode membrane proteins associated with smooth septate junctions (SSJ) which are required for intestinal barrier function. Based on bioinformatics analysis, dvssj1 appears to be an arthropod-specific gene. Diet based insect feeding assays using double-stranded RNA (dsRNA) targeting dvssj1 and dvssj2 demonstrate targeted mRNA suppression, larval growth inhibition, and mortality. In RNAi treated WCR, injury to the midgut was manifested by “blebbing” of the midgut epithelium into the gut lumen. Ultrastructural examination of midgut epithelial cells revealed apoptosis and regenerative activities. Transgenic plants expressing dsRNA targeting dvssj1 show insecticidal activity and significant plant protection from WCR damage. The data indicate that dvssj1 and dvssj2 are effective gene targets for the control of WCR using RNAi technology, by apparent suppression of production of their respective smooth septate junction membrane proteins located within the intestinal lining, leading to growth inhibition and mortality. Nature Publishing Group 2016-07-28 /pmc/articles/PMC4964579/ /pubmed/27464714 http://dx.doi.org/10.1038/srep30542 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Hu, Xu
Richtman, Nina M.
Zhao, Jian-Zhou
Duncan, Keith E.
Niu, Xiping
Procyk, Lisa A.
Oneal, Meghan A.
Kernodle, Bliss M.
Steimel, Joseph P.
Crane, Virginia C.
Sandahl, Gary
Ritland, Julie L.
Howard, Richard J.
Presnail, James K.
Lu, Albert L.
Wu, Gusui
Discovery of midgut genes for the RNA interference control of corn rootworm
title Discovery of midgut genes for the RNA interference control of corn rootworm
title_full Discovery of midgut genes for the RNA interference control of corn rootworm
title_fullStr Discovery of midgut genes for the RNA interference control of corn rootworm
title_full_unstemmed Discovery of midgut genes for the RNA interference control of corn rootworm
title_short Discovery of midgut genes for the RNA interference control of corn rootworm
title_sort discovery of midgut genes for the rna interference control of corn rootworm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964579/
https://www.ncbi.nlm.nih.gov/pubmed/27464714
http://dx.doi.org/10.1038/srep30542
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