Ferrocene‐Boronic Acid–Fructose Binding Based on Dual‐Plate Generator–Collector Voltammetry and Square‐Wave Voltammetry

The interaction of ferrocene‐boronic acid with fructose is investigated in aqueous 0.1 m phosphate buffer at pH 7, 8 and 9. Two voltammetric methods, based on 1) a dual‐plate generator–collector micro‐trench electrode (steady state) and 2) a square‐wave voltammetry (transient) method, are applied and compared in terms of mechanistic resolution. A combination of experimental data is employed to obtain new insights into the binding rates and the cumulative binding constants for both the reduced ferrocene‐boronic acid (pH dependent and weakly binding) and for the oxidised ferrocene‐boronic acid (pH independent and strongly binding).