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Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants
Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juven...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Associação Brasileira de Divulgação Científica
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964894/ https://www.ncbi.nlm.nih.gov/pubmed/27464022 http://dx.doi.org/10.1590/1414-431X20165281 |
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author | Pavan, T.R. Di Domenico, J. Kirsten, K.S. Nied, C.O. Frandoloso, R. Kreutz, L.C. |
author_facet | Pavan, T.R. Di Domenico, J. Kirsten, K.S. Nied, C.O. Frandoloso, R. Kreutz, L.C. |
author_sort | Pavan, T.R. |
collection | PubMed |
description | Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60–80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund’s complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at biweekly intervals. Our results demonstrated that CpGs ODNs and β-glucan were as effective as classical adjuvants (FCA, FIA, AlOH and Montanide) in promoting anti-BSA antibodies and that the kinetics of antibody production induced by all adjuvants used in our study had a similar trend to that observed in other fish species, with a peak at 28 days post-vaccination. These results may be useful for the selection of adjuvants for vaccine formulation intended for silver catfish and for the development of vaccine and vaccination strategies to other fish species. |
format | Online Article Text |
id | pubmed-4964894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Associação Brasileira de Divulgação Científica |
record_format | MEDLINE/PubMed |
spelling | pubmed-49648942016-08-10 Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants Pavan, T.R. Di Domenico, J. Kirsten, K.S. Nied, C.O. Frandoloso, R. Kreutz, L.C. Braz J Med Biol Res Biomedical Sciences Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60–80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund’s complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at biweekly intervals. Our results demonstrated that CpGs ODNs and β-glucan were as effective as classical adjuvants (FCA, FIA, AlOH and Montanide) in promoting anti-BSA antibodies and that the kinetics of antibody production induced by all adjuvants used in our study had a similar trend to that observed in other fish species, with a peak at 28 days post-vaccination. These results may be useful for the selection of adjuvants for vaccine formulation intended for silver catfish and for the development of vaccine and vaccination strategies to other fish species. Associação Brasileira de Divulgação Científica 2016-07-25 /pmc/articles/PMC4964894/ /pubmed/27464022 http://dx.doi.org/10.1590/1414-431X20165281 Text en http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License |
spellingShingle | Biomedical Sciences Pavan, T.R. Di Domenico, J. Kirsten, K.S. Nied, C.O. Frandoloso, R. Kreutz, L.C. Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants |
title | Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants |
title_full | Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants |
title_fullStr | Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants |
title_full_unstemmed | Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants |
title_short | Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants |
title_sort | antibody response in silver catfish (rhamdia quelen) immunized with a model antigen associated with different adjuvants |
topic | Biomedical Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4964894/ https://www.ncbi.nlm.nih.gov/pubmed/27464022 http://dx.doi.org/10.1590/1414-431X20165281 |
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