Cargando…
Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells
Glioma amplified sequence 41(GAS41) is a potent transcription factor that play a crucial role in cell proliferation and survival. In glioblastoma, the expression of GAS41 at both transcriptional and post transcriptional level needs to be tightly maintained in response to cellular signals. Micro RNAs...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4965126/ https://www.ncbi.nlm.nih.gov/pubmed/27467502 http://dx.doi.org/10.1371/journal.pone.0159092 |
_version_ | 1782445219049897984 |
---|---|
author | Pal, Dhananjaya Mukhopadhyay, Debasmita Ramaiah, M. Janaki Sarma, Pranjal Bhadra, Utpal Bhadra, Manika Pal |
author_facet | Pal, Dhananjaya Mukhopadhyay, Debasmita Ramaiah, M. Janaki Sarma, Pranjal Bhadra, Utpal Bhadra, Manika Pal |
author_sort | Pal, Dhananjaya |
collection | PubMed |
description | Glioma amplified sequence 41(GAS41) is a potent transcription factor that play a crucial role in cell proliferation and survival. In glioblastoma, the expression of GAS41 at both transcriptional and post transcriptional level needs to be tightly maintained in response to cellular signals. Micro RNAs (miRNA) are small non coding RNA that act as important regulators for modulating the expression of various target genes. Studies have shown that several miRNAs play role in the post-transcriptional regulation of GAS41. Here we identified GAS41 as a novel target for endogenous miR-203 and demonstrate an inverse correlation of miR-203 expression with GAS41 in glioma cell lines (HNGC2 and U87). Over expression of miR-203 negatively regulates GAS41 expression in U87 and HNGC2 cell lines. Moreover, miR-203 restrained miR-10b action by suppressing GAS41. GAS41 is essential for repressing p53 in tumor suppressor pathway during cell proliferation. Enforced expression of GAS41 produced contradictory effect on miR-203 but was able to enhance p53 tumor suppressor pathway associated protein. It was also found that miR-203 maintains the stability of p53 as knock down of p53 expression using siRNA resulted in down regulation of pri-miR and mature miR-203 expression. Conversely reconstitution of miR-203 expression induced apoptosis and inhibited migratory property of glioma cells. Taken together, we show that miR-203 is a key negative regulator of GAS41 and acts as tumor suppressor microRNA in glioma. |
format | Online Article Text |
id | pubmed-4965126 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-49651262016-08-18 Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells Pal, Dhananjaya Mukhopadhyay, Debasmita Ramaiah, M. Janaki Sarma, Pranjal Bhadra, Utpal Bhadra, Manika Pal PLoS One Research Article Glioma amplified sequence 41(GAS41) is a potent transcription factor that play a crucial role in cell proliferation and survival. In glioblastoma, the expression of GAS41 at both transcriptional and post transcriptional level needs to be tightly maintained in response to cellular signals. Micro RNAs (miRNA) are small non coding RNA that act as important regulators for modulating the expression of various target genes. Studies have shown that several miRNAs play role in the post-transcriptional regulation of GAS41. Here we identified GAS41 as a novel target for endogenous miR-203 and demonstrate an inverse correlation of miR-203 expression with GAS41 in glioma cell lines (HNGC2 and U87). Over expression of miR-203 negatively regulates GAS41 expression in U87 and HNGC2 cell lines. Moreover, miR-203 restrained miR-10b action by suppressing GAS41. GAS41 is essential for repressing p53 in tumor suppressor pathway during cell proliferation. Enforced expression of GAS41 produced contradictory effect on miR-203 but was able to enhance p53 tumor suppressor pathway associated protein. It was also found that miR-203 maintains the stability of p53 as knock down of p53 expression using siRNA resulted in down regulation of pri-miR and mature miR-203 expression. Conversely reconstitution of miR-203 expression induced apoptosis and inhibited migratory property of glioma cells. Taken together, we show that miR-203 is a key negative regulator of GAS41 and acts as tumor suppressor microRNA in glioma. Public Library of Science 2016-07-28 /pmc/articles/PMC4965126/ /pubmed/27467502 http://dx.doi.org/10.1371/journal.pone.0159092 Text en © 2016 Pal et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Pal, Dhananjaya Mukhopadhyay, Debasmita Ramaiah, M. Janaki Sarma, Pranjal Bhadra, Utpal Bhadra, Manika Pal Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells |
title | Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells |
title_full | Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells |
title_fullStr | Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells |
title_full_unstemmed | Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells |
title_short | Regulation of Cell Proliferation and Migration by miR-203 via GAS41/miR-10b Axis in Human Glioblastoma Cells |
title_sort | regulation of cell proliferation and migration by mir-203 via gas41/mir-10b axis in human glioblastoma cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4965126/ https://www.ncbi.nlm.nih.gov/pubmed/27467502 http://dx.doi.org/10.1371/journal.pone.0159092 |
work_keys_str_mv | AT paldhananjaya regulationofcellproliferationandmigrationbymir203viagas41mir10baxisinhumanglioblastomacells AT mukhopadhyaydebasmita regulationofcellproliferationandmigrationbymir203viagas41mir10baxisinhumanglioblastomacells AT ramaiahmjanaki regulationofcellproliferationandmigrationbymir203viagas41mir10baxisinhumanglioblastomacells AT sarmapranjal regulationofcellproliferationandmigrationbymir203viagas41mir10baxisinhumanglioblastomacells AT bhadrautpal regulationofcellproliferationandmigrationbymir203viagas41mir10baxisinhumanglioblastomacells AT bhadramanikapal regulationofcellproliferationandmigrationbymir203viagas41mir10baxisinhumanglioblastomacells |