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Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function
Mitofusin-2 (Mfn2), one of the mitochondrial dynamic proteins plays a key role in maintaining the integrity of mitochondrial morphology and function. However, it is unknown if Mfn2 influences the quality of oocytes in the process of development by modulating mitochondrial function in vitro. In this...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4965743/ https://www.ncbi.nlm.nih.gov/pubmed/27469431 http://dx.doi.org/10.1038/srep30561 |
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author | Liu, Qun Kang, Lina Wang, Lingjuan Zhang, Ling Xiang, Wenpei |
author_facet | Liu, Qun Kang, Lina Wang, Lingjuan Zhang, Ling Xiang, Wenpei |
author_sort | Liu, Qun |
collection | PubMed |
description | Mitofusin-2 (Mfn2), one of the mitochondrial dynamic proteins plays a key role in maintaining the integrity of mitochondrial morphology and function. However, it is unknown if Mfn2 influences the quality of oocytes in the process of development by modulating mitochondrial function in vitro. In this study, immature oocytes were transfected with Mfn2-siRNA for 16 h. We found that the expression level of the Mfn2 gene was significantly lower than those of the control group. The rates of maturation and fertility were also found to have declined. Moreover, mitochondrial structure and function, especially the morphogenesis of spindles, were observed as abnormal during meiosis. Thus, the above findings indicate that down-regulation of Mfn2 may have an impact on the maturation and fertilization of immature oocytes in vitro by modulating meiosis and mitochondrial function. |
format | Online Article Text |
id | pubmed-4965743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49657432016-08-08 Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function Liu, Qun Kang, Lina Wang, Lingjuan Zhang, Ling Xiang, Wenpei Sci Rep Article Mitofusin-2 (Mfn2), one of the mitochondrial dynamic proteins plays a key role in maintaining the integrity of mitochondrial morphology and function. However, it is unknown if Mfn2 influences the quality of oocytes in the process of development by modulating mitochondrial function in vitro. In this study, immature oocytes were transfected with Mfn2-siRNA for 16 h. We found that the expression level of the Mfn2 gene was significantly lower than those of the control group. The rates of maturation and fertility were also found to have declined. Moreover, mitochondrial structure and function, especially the morphogenesis of spindles, were observed as abnormal during meiosis. Thus, the above findings indicate that down-regulation of Mfn2 may have an impact on the maturation and fertilization of immature oocytes in vitro by modulating meiosis and mitochondrial function. Nature Publishing Group 2016-07-29 /pmc/articles/PMC4965743/ /pubmed/27469431 http://dx.doi.org/10.1038/srep30561 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Liu, Qun Kang, Lina Wang, Lingjuan Zhang, Ling Xiang, Wenpei Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function |
title | Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function |
title_full | Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function |
title_fullStr | Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function |
title_full_unstemmed | Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function |
title_short | Mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function |
title_sort | mitofusin 2 regulates the oocytes development and quality by modulating meiosis and mitochondrial function |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4965743/ https://www.ncbi.nlm.nih.gov/pubmed/27469431 http://dx.doi.org/10.1038/srep30561 |
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