Cargando…
CD271 regulates the proliferation and motility of hypopharyngeal cancer cells
CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2016
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4965829/ https://www.ncbi.nlm.nih.gov/pubmed/27469492 http://dx.doi.org/10.1038/srep30707 |
_version_ | 1782445323390550016 |
---|---|
author | Mochizuki, Mai Tamai, Keiichi Imai, Takayuki Sugawara, Sayuri Ogama, Naoko Nakamura, Mao Matsuura, Kazuto Yamaguchi, Kazunori Satoh, Kennichi Sato, Ikuro Motohashi, Hozumi Sugamura, Kazuo Tanaka, Nobuyuki |
author_facet | Mochizuki, Mai Tamai, Keiichi Imai, Takayuki Sugawara, Sayuri Ogama, Naoko Nakamura, Mao Matsuura, Kazuto Yamaguchi, Kazunori Satoh, Kennichi Sato, Ikuro Motohashi, Hozumi Sugamura, Kazuo Tanaka, Nobuyuki |
author_sort | Mochizuki, Mai |
collection | PubMed |
description | CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells’ tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G(0) and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G(0) arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC. |
format | Online Article Text |
id | pubmed-4965829 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-49658292016-08-08 CD271 regulates the proliferation and motility of hypopharyngeal cancer cells Mochizuki, Mai Tamai, Keiichi Imai, Takayuki Sugawara, Sayuri Ogama, Naoko Nakamura, Mao Matsuura, Kazuto Yamaguchi, Kazunori Satoh, Kennichi Sato, Ikuro Motohashi, Hozumi Sugamura, Kazuo Tanaka, Nobuyuki Sci Rep Article CD271 (p75 neurotrophin receptor) plays both positive and negative roles in cancer development, depending on the cell type. We previously reported that CD271 is a marker for tumor initiation and is correlated with a poor prognosis in human hypopharyngeal cancer (HPC). To clarify the role of CD271 in HPC, we established HPC cell lines and knocked down the CD271 expression using siRNA. We found that CD271-knockdown completely suppressed the cells’ tumor-forming capability both in vivo and in vitro. CD271-knockdown also induced cell-cycle arrest in G(0) and suppressed ERK phosphorylation. While treatment with an ERK inhibitor only partially inhibited cell growth, CDKN1C, which is required for maintenance of quiescence, was strongly upregulated in CD271-depleted HPC cells, and the double knockdown of CD271 and CDKN1C partially rescued the cells from G(0) arrest. In addition, either CD271 depletion or the inhibition of CD271-RhoA signaling by TAT-Pep5 diminished the in vitro migration capability of the HPC cells. Collectively, CD271 initiates tumor formation by increasing the cell proliferation capacity through CDKN1C suppression and ERK-signaling activation, and by accelerating the migration signaling pathway in HPC. Nature Publishing Group 2016-07-29 /pmc/articles/PMC4965829/ /pubmed/27469492 http://dx.doi.org/10.1038/srep30707 Text en Copyright © 2016, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Mochizuki, Mai Tamai, Keiichi Imai, Takayuki Sugawara, Sayuri Ogama, Naoko Nakamura, Mao Matsuura, Kazuto Yamaguchi, Kazunori Satoh, Kennichi Sato, Ikuro Motohashi, Hozumi Sugamura, Kazuo Tanaka, Nobuyuki CD271 regulates the proliferation and motility of hypopharyngeal cancer cells |
title | CD271 regulates the proliferation and motility of hypopharyngeal cancer cells |
title_full | CD271 regulates the proliferation and motility of hypopharyngeal cancer cells |
title_fullStr | CD271 regulates the proliferation and motility of hypopharyngeal cancer cells |
title_full_unstemmed | CD271 regulates the proliferation and motility of hypopharyngeal cancer cells |
title_short | CD271 regulates the proliferation and motility of hypopharyngeal cancer cells |
title_sort | cd271 regulates the proliferation and motility of hypopharyngeal cancer cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4965829/ https://www.ncbi.nlm.nih.gov/pubmed/27469492 http://dx.doi.org/10.1038/srep30707 |
work_keys_str_mv | AT mochizukimai cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT tamaikeiichi cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT imaitakayuki cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT sugawarasayuri cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT ogamanaoko cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT nakamuramao cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT matsuurakazuto cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT yamaguchikazunori cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT satohkennichi cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT satoikuro cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT motohashihozumi cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT sugamurakazuo cd271regulatestheproliferationandmotilityofhypopharyngealcancercells AT tanakanobuyuki cd271regulatestheproliferationandmotilityofhypopharyngealcancercells |