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Symmetry and scale orient Min protein patterns in shaped bacterial sculptures
The boundary of a cell defines the shape and scale for its subcellular organisation. However, the effects of the cell’s spatial boundaries as well as the geometry sensing and scale adaptation of intracellular molecular networks remain largely unexplored. Here, we show that living bacterial cells can...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966624/ https://www.ncbi.nlm.nih.gov/pubmed/26098227 http://dx.doi.org/10.1038/nnano.2015.126 |
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author | Wu, Fabai van Schie, Bas G.C. Keymer, Juan E. Dekker, Cees |
author_facet | Wu, Fabai van Schie, Bas G.C. Keymer, Juan E. Dekker, Cees |
author_sort | Wu, Fabai |
collection | PubMed |
description | The boundary of a cell defines the shape and scale for its subcellular organisation. However, the effects of the cell’s spatial boundaries as well as the geometry sensing and scale adaptation of intracellular molecular networks remain largely unexplored. Here, we show that living bacterial cells can be ‘sculpted’ into defined shapes, such as squares and rectangles, which are used to explore the spatial adaptation of Min proteins that oscillate pole-to-pole in rod-shape Escherichia coli to assist cell division. In a wide geometric parameter space, ranging from 2x1x1 to 11x6x1 μm(3), Min proteins exhibit versatile oscillation patterns, sustaining rotational, longitudinal, diagonal, stripe, and even transversal modes. These patterns are found to directly capture the symmetry and scale of the cell boundary, and the Min concentration gradients scale in adaptation to the cell size within a characteristic length range of 3–6 μm. Numerical simulations reveal that local microscopic Turing kinetics of Min proteins can yield global symmetry selection, gradient scaling, and an adaptive range, when and only when facilitated by the three-dimensional confinement of cell boundary. These findings cannot be explained by previous geometry-sensing models based on the longest distance, membrane area or curvature, and reveal that spatial boundaries can facilitate simple molecular interactions to result in far more versatile functions than previously understood. |
format | Online Article Text |
id | pubmed-4966624 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-49666242016-07-29 Symmetry and scale orient Min protein patterns in shaped bacterial sculptures Wu, Fabai van Schie, Bas G.C. Keymer, Juan E. Dekker, Cees Nat Nanotechnol Article The boundary of a cell defines the shape and scale for its subcellular organisation. However, the effects of the cell’s spatial boundaries as well as the geometry sensing and scale adaptation of intracellular molecular networks remain largely unexplored. Here, we show that living bacterial cells can be ‘sculpted’ into defined shapes, such as squares and rectangles, which are used to explore the spatial adaptation of Min proteins that oscillate pole-to-pole in rod-shape Escherichia coli to assist cell division. In a wide geometric parameter space, ranging from 2x1x1 to 11x6x1 μm(3), Min proteins exhibit versatile oscillation patterns, sustaining rotational, longitudinal, diagonal, stripe, and even transversal modes. These patterns are found to directly capture the symmetry and scale of the cell boundary, and the Min concentration gradients scale in adaptation to the cell size within a characteristic length range of 3–6 μm. Numerical simulations reveal that local microscopic Turing kinetics of Min proteins can yield global symmetry selection, gradient scaling, and an adaptive range, when and only when facilitated by the three-dimensional confinement of cell boundary. These findings cannot be explained by previous geometry-sensing models based on the longest distance, membrane area or curvature, and reveal that spatial boundaries can facilitate simple molecular interactions to result in far more versatile functions than previously understood. 2015-06-22 2015-08 /pmc/articles/PMC4966624/ /pubmed/26098227 http://dx.doi.org/10.1038/nnano.2015.126 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Wu, Fabai van Schie, Bas G.C. Keymer, Juan E. Dekker, Cees Symmetry and scale orient Min protein patterns in shaped bacterial sculptures |
title | Symmetry and scale orient Min protein patterns in shaped bacterial sculptures |
title_full | Symmetry and scale orient Min protein patterns in shaped bacterial sculptures |
title_fullStr | Symmetry and scale orient Min protein patterns in shaped bacterial sculptures |
title_full_unstemmed | Symmetry and scale orient Min protein patterns in shaped bacterial sculptures |
title_short | Symmetry and scale orient Min protein patterns in shaped bacterial sculptures |
title_sort | symmetry and scale orient min protein patterns in shaped bacterial sculptures |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966624/ https://www.ncbi.nlm.nih.gov/pubmed/26098227 http://dx.doi.org/10.1038/nnano.2015.126 |
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