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Clinical significance of TIPE expression in gastric carcinoma

BACKGROUND: TNFAIP8, also known as TIPE, is a suppressor of apoptosis. High expression of both TIPE mRNA and protein has been detected in various cancer cell lines and clinical specimens compared to healthy tissues. Many reports have shown that there is a strong correlation between TIPE overexpressi...

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Autores principales: Hu, Ruyi, Qiu, Xingfeng, Hong, Shifu, Meng, Luxi, Hong, Xinya, Qiu, Jinhua, Yang, Jingjing, Zhuang, Guohong, Liu, Zhongchen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966678/
https://www.ncbi.nlm.nih.gov/pubmed/27524904
http://dx.doi.org/10.2147/OTT.S100593
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author Hu, Ruyi
Qiu, Xingfeng
Hong, Shifu
Meng, Luxi
Hong, Xinya
Qiu, Jinhua
Yang, Jingjing
Zhuang, Guohong
Liu, Zhongchen
author_facet Hu, Ruyi
Qiu, Xingfeng
Hong, Shifu
Meng, Luxi
Hong, Xinya
Qiu, Jinhua
Yang, Jingjing
Zhuang, Guohong
Liu, Zhongchen
author_sort Hu, Ruyi
collection PubMed
description BACKGROUND: TNFAIP8, also known as TIPE, is a suppressor of apoptosis. High expression of both TIPE mRNA and protein has been detected in various cancer cell lines and clinical specimens compared to healthy tissues. Many reports have shown that there is a strong correlation between TIPE overexpression and cancer progression and poor prognosis in human solid cancers. METHODS: To illustrate the functional and clinical significance of TIPE in gastric cancer, we used reverse transcription polymerase chain reaction, quantitative real-time polymerase chain reaction, and immunohistochemistry to measure TIPE expression in clinical gastric specimens. Then, TIPE expression was knocked down by using shRNA and anti-DR5ScFv, to examine different expressions of TIPE in BGC823 cell lines, while cell proliferation and apoptosis were induced. RESULTS: We found that there was a strong correlation between TIPE expression and TNM stage (P=0.044), tumor depth (P=0.016), lymph node metastasis (P=0.026), and distant metastasis (P=0.045). No significant correlation was found between TIPE expression with the patients’ age (P=0.062) or sex (P=0.459). Anti-DR5ScFv induced TIPE depletion both in vitro and in vivo and resulted in apoptosis and suppression of proliferation. CONCLUSION: Our results suggested that TIPE expression was associated with gastric cancer progression, and most importantly, suppressing TIPE expression might be an effective therapeutic strategy.
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spelling pubmed-49666782016-08-12 Clinical significance of TIPE expression in gastric carcinoma Hu, Ruyi Qiu, Xingfeng Hong, Shifu Meng, Luxi Hong, Xinya Qiu, Jinhua Yang, Jingjing Zhuang, Guohong Liu, Zhongchen Onco Targets Ther Original Research BACKGROUND: TNFAIP8, also known as TIPE, is a suppressor of apoptosis. High expression of both TIPE mRNA and protein has been detected in various cancer cell lines and clinical specimens compared to healthy tissues. Many reports have shown that there is a strong correlation between TIPE overexpression and cancer progression and poor prognosis in human solid cancers. METHODS: To illustrate the functional and clinical significance of TIPE in gastric cancer, we used reverse transcription polymerase chain reaction, quantitative real-time polymerase chain reaction, and immunohistochemistry to measure TIPE expression in clinical gastric specimens. Then, TIPE expression was knocked down by using shRNA and anti-DR5ScFv, to examine different expressions of TIPE in BGC823 cell lines, while cell proliferation and apoptosis were induced. RESULTS: We found that there was a strong correlation between TIPE expression and TNM stage (P=0.044), tumor depth (P=0.016), lymph node metastasis (P=0.026), and distant metastasis (P=0.045). No significant correlation was found between TIPE expression with the patients’ age (P=0.062) or sex (P=0.459). Anti-DR5ScFv induced TIPE depletion both in vitro and in vivo and resulted in apoptosis and suppression of proliferation. CONCLUSION: Our results suggested that TIPE expression was associated with gastric cancer progression, and most importantly, suppressing TIPE expression might be an effective therapeutic strategy. Dove Medical Press 2016-07-25 /pmc/articles/PMC4966678/ /pubmed/27524904 http://dx.doi.org/10.2147/OTT.S100593 Text en © 2016 Hu et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Hu, Ruyi
Qiu, Xingfeng
Hong, Shifu
Meng, Luxi
Hong, Xinya
Qiu, Jinhua
Yang, Jingjing
Zhuang, Guohong
Liu, Zhongchen
Clinical significance of TIPE expression in gastric carcinoma
title Clinical significance of TIPE expression in gastric carcinoma
title_full Clinical significance of TIPE expression in gastric carcinoma
title_fullStr Clinical significance of TIPE expression in gastric carcinoma
title_full_unstemmed Clinical significance of TIPE expression in gastric carcinoma
title_short Clinical significance of TIPE expression in gastric carcinoma
title_sort clinical significance of tipe expression in gastric carcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966678/
https://www.ncbi.nlm.nih.gov/pubmed/27524904
http://dx.doi.org/10.2147/OTT.S100593
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