MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression

BACKGROUND: Brucella may establish chronic infection by regulating the expression of miRNAs. However, the role of miRNAs in modulating the intracellular growth of Brucella remains unclear. RESULTS: In this study, we show that Brucella. abortus infection leads to downregulation of miR-125b-5p in macr...

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Autores principales: Liu, Ning, Wang, Lin, Sun, Changjiang, Yang, Li, Sun, Wanchun, Peng, Qisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2016
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966734/
https://www.ncbi.nlm.nih.gov/pubmed/27473222
http://dx.doi.org/10.1186/s12866-016-0788-2
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author Liu, Ning
Wang, Lin
Sun, Changjiang
Yang, Li
Sun, Wanchun
Peng, Qisheng
author_facet Liu, Ning
Wang, Lin
Sun, Changjiang
Yang, Li
Sun, Wanchun
Peng, Qisheng
author_sort Liu, Ning
collection PubMed
description BACKGROUND: Brucella may establish chronic infection by regulating the expression of miRNAs. However, the role of miRNAs in modulating the intracellular growth of Brucella remains unclear. RESULTS: In this study, we show that Brucella. abortus infection leads to downregulation of miR-125b-5p in macrophages. We establish that miR-125b-5p targets A20, an inhibitor of the NF-kB activation. Additionally, expression of miR-125b-5p decreases A20 expression in B. abortus-infected macrophages and leads to NF-kB activation and increased production of TNFα. Furthermore, B. abortus survival is attenuated in the presence of miR-125b-5p. CONCLUSIONS: These results uncover a role for miR-125b-5p in the regulation of B. abortus intracellular survival via the control of A20 expression.
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spelling pubmed-49667342016-07-30 MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression Liu, Ning Wang, Lin Sun, Changjiang Yang, Li Sun, Wanchun Peng, Qisheng BMC Microbiol Research Article BACKGROUND: Brucella may establish chronic infection by regulating the expression of miRNAs. However, the role of miRNAs in modulating the intracellular growth of Brucella remains unclear. RESULTS: In this study, we show that Brucella. abortus infection leads to downregulation of miR-125b-5p in macrophages. We establish that miR-125b-5p targets A20, an inhibitor of the NF-kB activation. Additionally, expression of miR-125b-5p decreases A20 expression in B. abortus-infected macrophages and leads to NF-kB activation and increased production of TNFα. Furthermore, B. abortus survival is attenuated in the presence of miR-125b-5p. CONCLUSIONS: These results uncover a role for miR-125b-5p in the regulation of B. abortus intracellular survival via the control of A20 expression. BioMed Central 2016-07-29 /pmc/articles/PMC4966734/ /pubmed/27473222 http://dx.doi.org/10.1186/s12866-016-0788-2 Text en © The Author(s). 2016 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Liu, Ning
Wang, Lin
Sun, Changjiang
Yang, Li
Sun, Wanchun
Peng, Qisheng
MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression
title MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression
title_full MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression
title_fullStr MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression
title_full_unstemmed MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression
title_short MicroRNA-125b-5p suppresses Brucella abortus intracellular survival via control of A20 expression
title_sort microrna-125b-5p suppresses brucella abortus intracellular survival via control of a20 expression
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966734/
https://www.ncbi.nlm.nih.gov/pubmed/27473222
http://dx.doi.org/10.1186/s12866-016-0788-2
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