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Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells

Food allergy is a serious problem for infants and young children. Induction of antigen-specific oral tolerance is one therapeutic strategy. Enhancement of oral tolerance induction by diet is a promising strategy to prevent food allergy in infants. Thus, in this study, we evaluate the effect of probi...

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Autores principales: Aoki-Yoshida, Ayako, Yamada, Kiyoshi, Hachimura, Satoshi, Sashihara, Toshihiro, Ikegami, Shuji, Shimizu, Makoto, Totsuka, Mamoru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966961/
https://www.ncbi.nlm.nih.gov/pubmed/27472281
http://dx.doi.org/10.1371/journal.pone.0158643
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author Aoki-Yoshida, Ayako
Yamada, Kiyoshi
Hachimura, Satoshi
Sashihara, Toshihiro
Ikegami, Shuji
Shimizu, Makoto
Totsuka, Mamoru
author_facet Aoki-Yoshida, Ayako
Yamada, Kiyoshi
Hachimura, Satoshi
Sashihara, Toshihiro
Ikegami, Shuji
Shimizu, Makoto
Totsuka, Mamoru
author_sort Aoki-Yoshida, Ayako
collection PubMed
description Food allergy is a serious problem for infants and young children. Induction of antigen-specific oral tolerance is one therapeutic strategy. Enhancement of oral tolerance induction by diet is a promising strategy to prevent food allergy in infants. Thus, in this study, we evaluate the effect of probiotic Lactobacillus gasseri OLL2809 (LG2809) on oral tolerance induction in a mouse model. The degree of oral tolerance induction was evaluated by measuring the proliferation and level of IL-2 production of splenic CD4(+) T cells from DO11.10 mice fed ovalbumin (OVA) alone or OVA with LG2809. Oral administration of LG2809 significantly decreased the rate of proliferation and IL-2 production by CD4(+) T cells from OVA-fed mice. LG2809 increased a ratio of CD4(+) T-cell population, producing high levels of IL-10 and having strong suppressive activity. Moreover, LG2809 increased a ratio of plasmacytoid dendritic cells (pDCs) among the lamina propria (LP) in small intestine. When used as antigen presenting cells to naïve CD4(+) T cells from DO11.10 mice, LP cells from BALB/c mice fed LG2809 induced higher IL-10 production and stronger suppressive activity than those from non-treated mice. These results suggest that oral administration of LG2809 increases the population of pDCs in the LP, resulting in the enhancement of oral tolerance induction by increasing the ratio of effector regulatory T cells. LG2809 could, therefore, act as a potent immunomodulator to prevent food allergies by promoting oral tolerance.
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spelling pubmed-49669612016-08-18 Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells Aoki-Yoshida, Ayako Yamada, Kiyoshi Hachimura, Satoshi Sashihara, Toshihiro Ikegami, Shuji Shimizu, Makoto Totsuka, Mamoru PLoS One Research Article Food allergy is a serious problem for infants and young children. Induction of antigen-specific oral tolerance is one therapeutic strategy. Enhancement of oral tolerance induction by diet is a promising strategy to prevent food allergy in infants. Thus, in this study, we evaluate the effect of probiotic Lactobacillus gasseri OLL2809 (LG2809) on oral tolerance induction in a mouse model. The degree of oral tolerance induction was evaluated by measuring the proliferation and level of IL-2 production of splenic CD4(+) T cells from DO11.10 mice fed ovalbumin (OVA) alone or OVA with LG2809. Oral administration of LG2809 significantly decreased the rate of proliferation and IL-2 production by CD4(+) T cells from OVA-fed mice. LG2809 increased a ratio of CD4(+) T-cell population, producing high levels of IL-10 and having strong suppressive activity. Moreover, LG2809 increased a ratio of plasmacytoid dendritic cells (pDCs) among the lamina propria (LP) in small intestine. When used as antigen presenting cells to naïve CD4(+) T cells from DO11.10 mice, LP cells from BALB/c mice fed LG2809 induced higher IL-10 production and stronger suppressive activity than those from non-treated mice. These results suggest that oral administration of LG2809 increases the population of pDCs in the LP, resulting in the enhancement of oral tolerance induction by increasing the ratio of effector regulatory T cells. LG2809 could, therefore, act as a potent immunomodulator to prevent food allergies by promoting oral tolerance. Public Library of Science 2016-07-29 /pmc/articles/PMC4966961/ /pubmed/27472281 http://dx.doi.org/10.1371/journal.pone.0158643 Text en © 2016 Aoki-Yoshida et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Aoki-Yoshida, Ayako
Yamada, Kiyoshi
Hachimura, Satoshi
Sashihara, Toshihiro
Ikegami, Shuji
Shimizu, Makoto
Totsuka, Mamoru
Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells
title Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells
title_full Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells
title_fullStr Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells
title_full_unstemmed Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells
title_short Enhancement of Oral Tolerance Induction in DO11.10 Mice by Lactobacillus gasseri OLL2809 via Increase of Effector Regulatory T Cells
title_sort enhancement of oral tolerance induction in do11.10 mice by lactobacillus gasseri oll2809 via increase of effector regulatory t cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966961/
https://www.ncbi.nlm.nih.gov/pubmed/27472281
http://dx.doi.org/10.1371/journal.pone.0158643
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