Cargando…

Identification of TENP as the Gene Encoding Chicken Egg White Ovoglobulin G2 and Demonstration of Its High Genetic Variability in Chickens

Ovoglobulin G2 (G2) has long been known as a major protein constituent of chicken egg white. However, little is known about the biochemical properties and biological functions of G2 because the gene encoding G2 has not been identified. Therefore, the identification of the gene encoding G2 and an ana...

Descripción completa

Detalles Bibliográficos
Autores principales: Kinoshita, Keiji, Shimogiri, Takeshi, Ibrahim, Hisham R., Tsudzuki, Masaoki, Maeda, Yoshizane, Matsuda, Yoichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966965/
https://www.ncbi.nlm.nih.gov/pubmed/27472057
http://dx.doi.org/10.1371/journal.pone.0159571
_version_ 1782445464138809344
author Kinoshita, Keiji
Shimogiri, Takeshi
Ibrahim, Hisham R.
Tsudzuki, Masaoki
Maeda, Yoshizane
Matsuda, Yoichi
author_facet Kinoshita, Keiji
Shimogiri, Takeshi
Ibrahim, Hisham R.
Tsudzuki, Masaoki
Maeda, Yoshizane
Matsuda, Yoichi
author_sort Kinoshita, Keiji
collection PubMed
description Ovoglobulin G2 (G2) has long been known as a major protein constituent of chicken egg white. However, little is known about the biochemical properties and biological functions of G2 because the gene encoding G2 has not been identified. Therefore, the identification of the gene encoding G2 and an analysis of its genetic variability is an important step toward the goal of understanding the biological functions of the G2 protein and its utility in poultry production. To identify and characterize the gene encoding G2, we separated G2 from egg white using electrophoresis on a non-denaturing polyacrylamide gel. Two polymorphic forms of G2 protein (G2(A) and G2(B)), with different mobilities (fast and slow respectively), were detected by staining. The protein band corresponding to G2(B) was electro-eluted from the native gel, re-electrophoresed under denaturing conditions and its N-terminal sequence was determined by Edman degradation following transfer onto a membrane. Sequencing of the 47 kDa G2(B) band revealed it to be identical to TENP (transiently expressed in neural precursors), also known as BPI fold-containing family B, member 2 (BPIFB2), a protein with strong homology to a bacterial permeability-increasing protein family (BPI) in mammals. Full-length chicken TENP cDNA sequences were determined for 78 individuals across 29 chicken breeds, lines, and populations, and consequently eleven non-synonymous substitutions were detected in the coding region. Of the eleven non-synonymous substitutions, A329G leading to Arg110Gln was completely associated with the noted differential electrophoretic mobility of G2. Specifically G2(B), with a slower mobility is encoded by A329 (Arg110), whereas G2(A), with a faster mobility, is encoded by G329 (Gln110). The sequence data, derived from the coding region, also revealed that the gene encoding G2 demonstrates significant genetic variability across different chicken breeds/lines/populations. These variants, and how they correlate with egg white properties, may allow us to understand further G2’s functions.
format Online
Article
Text
id pubmed-4966965
institution National Center for Biotechnology Information
language English
publishDate 2016
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-49669652016-08-18 Identification of TENP as the Gene Encoding Chicken Egg White Ovoglobulin G2 and Demonstration of Its High Genetic Variability in Chickens Kinoshita, Keiji Shimogiri, Takeshi Ibrahim, Hisham R. Tsudzuki, Masaoki Maeda, Yoshizane Matsuda, Yoichi PLoS One Research Article Ovoglobulin G2 (G2) has long been known as a major protein constituent of chicken egg white. However, little is known about the biochemical properties and biological functions of G2 because the gene encoding G2 has not been identified. Therefore, the identification of the gene encoding G2 and an analysis of its genetic variability is an important step toward the goal of understanding the biological functions of the G2 protein and its utility in poultry production. To identify and characterize the gene encoding G2, we separated G2 from egg white using electrophoresis on a non-denaturing polyacrylamide gel. Two polymorphic forms of G2 protein (G2(A) and G2(B)), with different mobilities (fast and slow respectively), were detected by staining. The protein band corresponding to G2(B) was electro-eluted from the native gel, re-electrophoresed under denaturing conditions and its N-terminal sequence was determined by Edman degradation following transfer onto a membrane. Sequencing of the 47 kDa G2(B) band revealed it to be identical to TENP (transiently expressed in neural precursors), also known as BPI fold-containing family B, member 2 (BPIFB2), a protein with strong homology to a bacterial permeability-increasing protein family (BPI) in mammals. Full-length chicken TENP cDNA sequences were determined for 78 individuals across 29 chicken breeds, lines, and populations, and consequently eleven non-synonymous substitutions were detected in the coding region. Of the eleven non-synonymous substitutions, A329G leading to Arg110Gln was completely associated with the noted differential electrophoretic mobility of G2. Specifically G2(B), with a slower mobility is encoded by A329 (Arg110), whereas G2(A), with a faster mobility, is encoded by G329 (Gln110). The sequence data, derived from the coding region, also revealed that the gene encoding G2 demonstrates significant genetic variability across different chicken breeds/lines/populations. These variants, and how they correlate with egg white properties, may allow us to understand further G2’s functions. Public Library of Science 2016-07-29 /pmc/articles/PMC4966965/ /pubmed/27472057 http://dx.doi.org/10.1371/journal.pone.0159571 Text en © 2016 Kinoshita et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Kinoshita, Keiji
Shimogiri, Takeshi
Ibrahim, Hisham R.
Tsudzuki, Masaoki
Maeda, Yoshizane
Matsuda, Yoichi
Identification of TENP as the Gene Encoding Chicken Egg White Ovoglobulin G2 and Demonstration of Its High Genetic Variability in Chickens
title Identification of TENP as the Gene Encoding Chicken Egg White Ovoglobulin G2 and Demonstration of Its High Genetic Variability in Chickens
title_full Identification of TENP as the Gene Encoding Chicken Egg White Ovoglobulin G2 and Demonstration of Its High Genetic Variability in Chickens
title_fullStr Identification of TENP as the Gene Encoding Chicken Egg White Ovoglobulin G2 and Demonstration of Its High Genetic Variability in Chickens
title_full_unstemmed Identification of TENP as the Gene Encoding Chicken Egg White Ovoglobulin G2 and Demonstration of Its High Genetic Variability in Chickens
title_short Identification of TENP as the Gene Encoding Chicken Egg White Ovoglobulin G2 and Demonstration of Its High Genetic Variability in Chickens
title_sort identification of tenp as the gene encoding chicken egg white ovoglobulin g2 and demonstration of its high genetic variability in chickens
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966965/
https://www.ncbi.nlm.nih.gov/pubmed/27472057
http://dx.doi.org/10.1371/journal.pone.0159571
work_keys_str_mv AT kinoshitakeiji identificationoftenpasthegeneencodingchickeneggwhiteovoglobuling2anddemonstrationofitshighgeneticvariabilityinchickens
AT shimogiritakeshi identificationoftenpasthegeneencodingchickeneggwhiteovoglobuling2anddemonstrationofitshighgeneticvariabilityinchickens
AT ibrahimhishamr identificationoftenpasthegeneencodingchickeneggwhiteovoglobuling2anddemonstrationofitshighgeneticvariabilityinchickens
AT tsudzukimasaoki identificationoftenpasthegeneencodingchickeneggwhiteovoglobuling2anddemonstrationofitshighgeneticvariabilityinchickens
AT maedayoshizane identificationoftenpasthegeneencodingchickeneggwhiteovoglobuling2anddemonstrationofitshighgeneticvariabilityinchickens
AT matsudayoichi identificationoftenpasthegeneencodingchickeneggwhiteovoglobuling2anddemonstrationofitshighgeneticvariabilityinchickens