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Profilin connects actin assembly with microtubule dynamics

Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making act...

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Autores principales: Nejedla, Michaela, Sadi, Sara, Sulimenko, Vadym, de Almeida, Francisca Nunes, Blom, Hans, Draber, Pavel, Aspenström, Pontus, Karlsson, Roger
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2016
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966980/
https://www.ncbi.nlm.nih.gov/pubmed/27307590
http://dx.doi.org/10.1091/mbc.E15-11-0799
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author Nejedla, Michaela
Sadi, Sara
Sulimenko, Vadym
de Almeida, Francisca Nunes
Blom, Hans
Draber, Pavel
Aspenström, Pontus
Karlsson, Roger
author_facet Nejedla, Michaela
Sadi, Sara
Sulimenko, Vadym
de Almeida, Francisca Nunes
Blom, Hans
Draber, Pavel
Aspenström, Pontus
Karlsson, Roger
author_sort Nejedla, Michaela
collection PubMed
description Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making actin polymerization from microtubule-associated platforms possible. Microtubules are implicated in focal adhesion turnover, cell polarity establishment, and migration, illustrating the coupling between actin and microtubule systems. Here we demonstrate that profilin is functionally linked to microtubules with formins and point to formins as major mediators of this association. To reach this conclusion, we combined different fluorescence microscopy techniques, including superresolution microscopy, with siRNA modulation of profilin expression and drug treatments to interfere with actin dynamics. Our studies show that profilin dynamically associates with microtubules and this fraction of profilin contributes to balance actin assembly during homeostatic cell growth and affects micro­tubule dynamics. Hence profilin functions as a regulator of microtubule (+)-end turnover in addition to being an actin control element.
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spelling pubmed-49669802016-10-16 Profilin connects actin assembly with microtubule dynamics Nejedla, Michaela Sadi, Sara Sulimenko, Vadym de Almeida, Francisca Nunes Blom, Hans Draber, Pavel Aspenström, Pontus Karlsson, Roger Mol Biol Cell Articles Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making actin polymerization from microtubule-associated platforms possible. Microtubules are implicated in focal adhesion turnover, cell polarity establishment, and migration, illustrating the coupling between actin and microtubule systems. Here we demonstrate that profilin is functionally linked to microtubules with formins and point to formins as major mediators of this association. To reach this conclusion, we combined different fluorescence microscopy techniques, including superresolution microscopy, with siRNA modulation of profilin expression and drug treatments to interfere with actin dynamics. Our studies show that profilin dynamically associates with microtubules and this fraction of profilin contributes to balance actin assembly during homeostatic cell growth and affects micro­tubule dynamics. Hence profilin functions as a regulator of microtubule (+)-end turnover in addition to being an actin control element. The American Society for Cell Biology 2016-08-01 /pmc/articles/PMC4966980/ /pubmed/27307590 http://dx.doi.org/10.1091/mbc.E15-11-0799 Text en © 2016 Nejedla et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Nejedla, Michaela
Sadi, Sara
Sulimenko, Vadym
de Almeida, Francisca Nunes
Blom, Hans
Draber, Pavel
Aspenström, Pontus
Karlsson, Roger
Profilin connects actin assembly with microtubule dynamics
title Profilin connects actin assembly with microtubule dynamics
title_full Profilin connects actin assembly with microtubule dynamics
title_fullStr Profilin connects actin assembly with microtubule dynamics
title_full_unstemmed Profilin connects actin assembly with microtubule dynamics
title_short Profilin connects actin assembly with microtubule dynamics
title_sort profilin connects actin assembly with microtubule dynamics
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966980/
https://www.ncbi.nlm.nih.gov/pubmed/27307590
http://dx.doi.org/10.1091/mbc.E15-11-0799
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