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Profilin connects actin assembly with microtubule dynamics
Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making act...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The American Society for Cell Biology
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966980/ https://www.ncbi.nlm.nih.gov/pubmed/27307590 http://dx.doi.org/10.1091/mbc.E15-11-0799 |
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author | Nejedla, Michaela Sadi, Sara Sulimenko, Vadym de Almeida, Francisca Nunes Blom, Hans Draber, Pavel Aspenström, Pontus Karlsson, Roger |
author_facet | Nejedla, Michaela Sadi, Sara Sulimenko, Vadym de Almeida, Francisca Nunes Blom, Hans Draber, Pavel Aspenström, Pontus Karlsson, Roger |
author_sort | Nejedla, Michaela |
collection | PubMed |
description | Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making actin polymerization from microtubule-associated platforms possible. Microtubules are implicated in focal adhesion turnover, cell polarity establishment, and migration, illustrating the coupling between actin and microtubule systems. Here we demonstrate that profilin is functionally linked to microtubules with formins and point to formins as major mediators of this association. To reach this conclusion, we combined different fluorescence microscopy techniques, including superresolution microscopy, with siRNA modulation of profilin expression and drug treatments to interfere with actin dynamics. Our studies show that profilin dynamically associates with microtubules and this fraction of profilin contributes to balance actin assembly during homeostatic cell growth and affects microtubule dynamics. Hence profilin functions as a regulator of microtubule (+)-end turnover in addition to being an actin control element. |
format | Online Article Text |
id | pubmed-4966980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2016 |
publisher | The American Society for Cell Biology |
record_format | MEDLINE/PubMed |
spelling | pubmed-49669802016-10-16 Profilin connects actin assembly with microtubule dynamics Nejedla, Michaela Sadi, Sara Sulimenko, Vadym de Almeida, Francisca Nunes Blom, Hans Draber, Pavel Aspenström, Pontus Karlsson, Roger Mol Biol Cell Articles Profilin controls actin nucleation and assembly processes in eukaryotic cells. Actin nucleation and elongation promoting factors (NEPFs) such as Ena/VASP, formins, and WASP-family proteins recruit profilin:actin for filament formation. Some of these are found to be microtubule associated, making actin polymerization from microtubule-associated platforms possible. Microtubules are implicated in focal adhesion turnover, cell polarity establishment, and migration, illustrating the coupling between actin and microtubule systems. Here we demonstrate that profilin is functionally linked to microtubules with formins and point to formins as major mediators of this association. To reach this conclusion, we combined different fluorescence microscopy techniques, including superresolution microscopy, with siRNA modulation of profilin expression and drug treatments to interfere with actin dynamics. Our studies show that profilin dynamically associates with microtubules and this fraction of profilin contributes to balance actin assembly during homeostatic cell growth and affects microtubule dynamics. Hence profilin functions as a regulator of microtubule (+)-end turnover in addition to being an actin control element. The American Society for Cell Biology 2016-08-01 /pmc/articles/PMC4966980/ /pubmed/27307590 http://dx.doi.org/10.1091/mbc.E15-11-0799 Text en © 2016 Nejedla et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. |
spellingShingle | Articles Nejedla, Michaela Sadi, Sara Sulimenko, Vadym de Almeida, Francisca Nunes Blom, Hans Draber, Pavel Aspenström, Pontus Karlsson, Roger Profilin connects actin assembly with microtubule dynamics |
title | Profilin connects actin assembly with microtubule dynamics |
title_full | Profilin connects actin assembly with microtubule dynamics |
title_fullStr | Profilin connects actin assembly with microtubule dynamics |
title_full_unstemmed | Profilin connects actin assembly with microtubule dynamics |
title_short | Profilin connects actin assembly with microtubule dynamics |
title_sort | profilin connects actin assembly with microtubule dynamics |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4966980/ https://www.ncbi.nlm.nih.gov/pubmed/27307590 http://dx.doi.org/10.1091/mbc.E15-11-0799 |
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