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Latitude in sample handling and storage for infant faecal microbiota studies: the elephant in the room?
BACKGROUND: In this manuscript, we investigate the “stones best left unturned” of sample storage and preparation and their implications for the next-generation sequencing of infant faecal microbial communities by the 16S ribosomal ribonucleic acid (rRNA) gene. We present a number of experiments that...
Autores principales: | Shaw, Alexander G., Sim, Kathleen, Powell, Elizabeth, Cornwell, Emma, Cramer, Teresa, McClure, Zoë E., Li, Ming-Shi, Kroll, J. Simon |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2016
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4967342/ https://www.ncbi.nlm.nih.gov/pubmed/27473284 http://dx.doi.org/10.1186/s40168-016-0186-x |
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